Abstract:
:Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vitro study, which combined RNase footprinting, gel shift binding assays, and processing assays, to investigate the molecular basis and function of the interaction between the native let-7g precursor (pre-let-7g) and LIN28. We have mapped the structure of pre-let-7g and identified some regions of the terminal loop of pre-let-7g that physically interact with LIN28. We have also identified a conformational change upon LIN28 binding that results in the unwinding of an otherwise double-stranded region at the Dicer processing site of pre-let-7g. Furthermore, we showed that a mutant pre-let-7g that displays an open upper stem inhibited pre-let-7g Dicer processing to the same extent as LIN28. The data support a mechanism by which LIN28 can directly inhibit let-7g biogenesis at the Dicer processing step.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lightfoot HL,Bugaut A,Armisen J,Lehrbach NJ,Miska EA,Balasubramanian Sdoi
10.1021/bi200851dsubject
Has Abstractpub_date
2011-09-06 00:00:00pages
7514-21issue
35eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi2017014
更新日期:2012-01-10 00:00:00
abstract::Modelin-5 isoforms were used to gain an insight into the effects of amidation on antimicrobial selectivity. When tested against Escherichia coli, amidation increased toxicity 10-fold (MIC = 31.25 μM) while showing limited increased hemolytic activity (2% lysis). Our results show that both the amidated and non-amidated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201267v
更新日期:2011-12-20 00:00:00
abstract::Insulin decreases multifunctional protein kinase (MFPK) activity in rat adipose tissue [Ramakrishna, S., & Benjamin, W. B. (1988) J. Biol. Chem. 263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). The mechanism for thi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00428a067
更新日期:1989-01-24 00:00:00
abstract::SHP-1, a protein tyrosine phosphatase containing two tandem SH2 domains, is autoinhibited at rest by its N-terminal SH2 (N-SH2) domain. Relief from autoinhibition and a subsequent 10-60-fold increase in V(max) have been observed upon N-SH2 domain engagement by a specific phosphotyrosyl ligand or upon deletion of the S...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9611873
更新日期:1997-03-11 00:00:00
abstract::Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00844
更新日期:2019-11-19 00:00:00
abstract::Ketosteroid isomerase (KSI) from Pseudomonas putida biotype B is a homodimeric enzyme catalyzing an allylic rearrangement of Delta5-3-ketosteroids at rates comparable with the diffusion-controlled limit. The tyrosine triad (Tyr14.Tyr55.Tyr30) forming a hydrogen-bond network in the apolar active site of KSI has been ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015547k
更新日期:2001-11-13 00:00:00
abstract::Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00684a010
更新日期:1975-07-01 00:00:00
abstract::Human fibrinogen gamma-module comprising residues gamma148-411 was expressed in Escherichia coli and refolded in vitro. Differential scanning calorimetry revealed that in addition to the two previously identified independently folded thermolabile domains, one in each half of the module, the gamma-module also contains ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962795l
更新日期:1997-04-15 00:00:00
abstract::The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a023
更新日期:1994-05-31 00:00:00
abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300429p
更新日期:2012-06-12 00:00:00
abstract::The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001071d
更新日期:2000-12-12 00:00:00
abstract::The interactions of bovine brain MAP2 and tau, recombinant murine MAP2C, and recombinant human tau with phosphatidylinositol vesicles yield apparent Kd values of 51 +/- 6 nM, 2.4 +/- 0.6 microM, 1.4 +/- 0.1 microM, and 1.6 +/- 0.2 microM, respectively. Examinations of the binding of MAP2 and/or MAP2C to phosphatidylch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00192a009
更新日期:1994-07-05 00:00:00
abstract::The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002646+
更新日期:2001-03-27 00:00:00
abstract::ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900760n
更新日期:2009-10-27 00:00:00
abstract::Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971045o
更新日期:1997-11-04 00:00:00
abstract::Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971276v
更新日期:1997-10-21 00:00:00
abstract::The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036269n
更新日期:2004-03-02 00:00:00
abstract::Multimodular enzymes, including polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and mixed PKS/NRPS systems, contain functional domains with similar functions. Domain swapping and module fusion are potential powerful strategies for creating hybrid enzymes to synthesize modified natural products. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047538s
更新日期:2005-03-29 00:00:00
abstract::Ricin A-chain (RTA) catalyzes the hydrolytic depurination of a specific adenosine at position 4324 of 28S rRNA. Kinetic isotope effects on the hydrolysis of a small 10mer stem-tetraloop oligonucleotide substrate established the mechanism of the reaction as D(N)*A(N), involving an oxacarbenium ion intermediate in a hig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0498499
更新日期:2004-05-04 00:00:00
abstract::ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701933q
更新日期:2008-04-15 00:00:00
abstract::Modification of pig kidney fructose-1,6-bisphosphatase with 2,3-butanedione in borate buffer (pH 7.8) leads to the loss of the activation of the enzyme by monovalent cations, as well as to the loss of allosteric adenosine 5'-monophosphate (AMP) inhibition. In agreement with the results obtained for the butanedione mod...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00661a017
更新日期:1976-08-10 00:00:00
abstract::The causes of the salting-in of beta-lactoglobulin by glycine and NaCl, a solubility behavior contrary to expectations, were probed by a detailed study of the interactions between these solvent components and the protein. The preferential interactions of beta-lactoglobulin with solvent components in aqueous glycine an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00390a038
更新日期:1987-08-11 00:00:00
abstract::Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201450v
更新日期:2011-12-20 00:00:00
abstract::Acarbose is a naturally occurring pseudo-tetrasaccharide. It has been used in conjunction with other drugs in the treatment of diabetes where it acts as an inhibitor of intestinal glucosidases. To probe the interactions of acarbose with other carbohydrate recognition enzymes, the crystal structure of E. coli maltodext...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9828573
更新日期:1999-04-27 00:00:00
abstract::An inhibitor of the insulin receptor tyrosine kinase (IRTK), (hydroxy-2-naphthalenyl-methyl) phosphonic acid, was designed and synthesized and was shown to be an inhibitor of the biological effects of insulin in vitro. With a wheat germ purified human placental insulin receptor preparation, this compound inhibited the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a053
更新日期:1989-06-27 00:00:00
abstract::The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961598j
更新日期:1996-12-17 00:00:00
abstract::3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00255a018
更新日期:1994-12-27 00:00:00
abstract::The development of inhibitors of insulin-regulated aminopeptidase (IRAP), a membrane-bound zinc metallopeptidase, is a promising approach for the discovery of drugs for the treatment of memory loss such as that associated with Alzheimer's disease. There is, however, no consensus in the literature about the mechanism b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101893w
更新日期:2011-04-05 00:00:00
abstract::The DNA binding domain (DBD) of p53 folds by a complex mechanism that involves parallel pathways and multiple intermediates, both on- and off-pathway. This heterogeneity renders DBD particularly susceptible to misfolding and aggregation. The origins of parallel folding mechanisms are not well understood. DBD folding h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062106y
更新日期:2007-03-13 00:00:00
abstract::The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048614u
更新日期:2004-11-02 00:00:00