Effects of guanidine hydrochloride on the proton inventory of proteins: implications on interpretations of protein stability.

abstract：:The trifunctional dinuclear platinum compounds 1,2/c,c [[cis-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) and 1,2/t,c [[trans-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) contain a monofunctional platinum coordination sphere linked to a cis-[PtCl(2)(amine)(2)] moiety. The compounds ...

journal_title：Biochemistry

authors： Kloster M,Kostrhunova H,Zaludova R,Malina J,Kasparkova J,Brabec V,Farrell N

更新日期：2004-06-22 00:00:00

abstract：:The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...

journal_title：Biochemistry

authors： Pfeiffer DR,Hutson SM,Kauffman RF,Lardy HA

更新日期：1976-06-15 00:00:00

abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

journal_title：Biochemistry

authors： Koga K,Berliner LJ

更新日期：1985-12-03 00:00:00

abstract：:The carboxy-terminal domain of the alpha subunit of Escherichia coli RNA polymerase, which is connected with the core part of RNA polymerase through a long flexible linker, plays decisive roles in transcription activation by directly interacting with a large number of transcription factors and upstream (UP) element DN...

journal_title：Biochemistry

authors： Fujita N,Endo S,Ishihama A

更新日期：2000-05-23 00:00:00

abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

journal_title：Biochemistry

authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

更新日期：2015-01-20 00:00:00

abstract：:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...

journal_title：Biochemistry

authors： Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

更新日期：1989-05-02 00:00:00

abstract：:The simplest carbapenem antibiotic, (5R)-carbapen-2-em-3-carboxylic acid, is biosynthesized from primary metabolites in Pectobacterium carotorova by the action of three enzymes, carboxymethylproline synthase (hereafter named CarB), carbapenam synthetase, and carbapenem synthase. CarB, a member of the crotonase superfa...

journal_title：Biochemistry

authors： Gerratana B,Arnett SO,Stapon A,Townsend CA

更新日期：2004-12-21 00:00:00

abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

journal_title：Biochemistry

authors： Wettich A,Biebricher CK

更新日期：2001-03-20 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site ...

journal_title：Biochemistry

authors： Linse S,Bylsma NR,Drakenberg T,Sellers P,Forsén S,Thulin E,Svensson LA,Zajtzeva I,Zajtsev V,Marek J

更新日期：1994-10-18 00:00:00

abstract：:The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase ...

journal_title：Biochemistry

authors： Carlson GM,Graves DJ

更新日期：1976-10-05 00:00:00

abstract：:During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...

journal_title：Biochemistry

authors： Casy W,Prater AR,Cornish PV

更新日期：2018-04-03 00:00:00

abstract：:Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/...

journal_title：Biochemistry

authors： Venters RA,Calderone TL,Spicer LD,Fierke CA

更新日期：1991-05-07 00:00:00

abstract：:The complete sequence of histone 2B of Drosophila has been determined by using an improved Beckman sequenator. Comparing these data with those previously published by other investigators on the histone 2B of calf [Iwai, K., Hayashi, H., & Ishikawa, K. (1972) J. Biochem. (Tokyo) 72, 357--367], trout [Koostra, A., & Bai...

journal_title：Biochemistry

authors： Elgin SC,Schilling J,Hood LE

更新日期：1979-12-11 00:00:00

abstract：:The side chains of the interacting pair Asp237(helix VII)-Lys358(helix XI) or Asp240(helix VII)-Lys319(helix X) in the lactose permease of Escherichia coli were extended by replacement with Glu and/or Arg or by site-specific derivatization of single-Cys replacement mutants. Iodoacetic acid was used to carboxymethylate...

journal_title：Biochemistry

authors： Sahin-Tóth M,Kaback HR

更新日期：1993-09-28 00:00:00

abstract：:Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...

journal_title：Biochemistry

authors： Warren JC,Murdock GL,Ma Y,Goodman SR,Zimmer WE

更新日期：1993-02-16 00:00:00

abstract：:Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be ...

journal_title：Biochemistry

authors： Kean JM,Murakami A,Blake KR,Cushman CD,Miller PS

更新日期：1988-12-27 00:00:00

abstract：:We have measured the pH-dependent (1)H, (13)C, and (15)N NMR spectra of pyridoxal 5'-phosphate ((13)C(2)-PLP) mixed with equal amounts of either doubly (15)N-labeled diaminopropane, (15)N(α)-labeled l-lysine, or (15)N(ε)-labeled l-lysine as model systems for various intermediates of the transimination reaction in PLP-...

journal_title：Biochemistry

authors： Chan-Huot M,Sharif S,Tolstoy PM,Toney MD,Limbach HH

更新日期：2010-12-28 00:00:00

abstract：:Crystallographic studies have demonstrated two flavin conformations for p-hydroxybenzoate hydroxylase (PHBH) [Gatti, D. L., Palfey, B. A. , Lah, M. S., Entsch, B., Massey, V., Ballou, D. P., & Ludwig, M. L. (1994) Science 266, 110-114. Schreuder, H. A., Mattevi, A., Obmolova, G., Kalk, K. H., Hol, W. G. J., van der Bo...

journal_title：Biochemistry

authors： Palfey BA,Ballou DP,Massey V

更新日期：1997-12-16 00:00:00

abstract：:The solution structure and dynamics of human insulin are investigated by 2D 1H NMR spectroscopy in reference to a previously analyzed analogue, des-pentapeptide(B26-B30) insulin (DPI; Hua, Q.X., & Weiss, M.A. (1990) Biochemistry 29, 10545-10555). This spectroscopic comparison is of interest since (i) the structure of ...

journal_title：Biochemistry

authors： Hua QX,Weiss MA

更新日期：1991-06-04 00:00:00

abstract：:A method is presented for the rapid determination of substrate selection in a manner that is not restricted to conditions of metabolic and isotopic steady state. Competition between several substrates can be assessed directly and continuously in a single experiment, allowing the effect of interventions to be studied. ...

journal_title：Biochemistry

authors： Malloy CR,Thompson JR,Jeffrey FM,Sherry AD

更新日期：1990-07-24 00:00:00

abstract：:The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...

journal_title：Biochemistry

authors： Juszczak LJ,Desamero RZ

更新日期：2009-03-31 00:00:00

abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2012-07-31 00:00:00

abstract：:We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...

journal_title：Biochemistry

authors： Karantza V,Freire E,Moudrianakis EN

更新日期：2001-10-30 00:00:00

abstract：:Centrioles are essential components of the animal centrosome and play crucial roles in the formation of cilia and flagella. They are cylindrical structures composed of nine triplet microtubules organized around a central cartwheel. Recent studies have identified spindle assembly abnormal protein SAS-6 as a critical co...

journal_title：Biochemistry

authors： Gupta H,Badarudeen B,George A,Thomas GE,Gireesh KK,Manna TK

更新日期：2015-10-20 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...

journal_title：Biochemistry

authors： Holzbaur IE,English AM,Ismail AA

更新日期：1996-04-30 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...

journal_title：Biochemistry

authors： Danyal K,Dean DR,Hoffman BM,Seefeldt LC

更新日期：2011-11-01 00:00:00

abstract：:The introduction into peptide chains of alpha-aminoisobutyric acid (Aib) has proven to stabilize the helical structure in short peptides by restricting the available range of polypeptide backbone conformations. In order to evaluate the potential stabilizing effect of Aib at the protein level, we have studied the confo...

journal_title：Biochemistry

authors： De Filippis V,De Antoni F,Frigo M,Polverino de Laureto P,Fontana A

更新日期：1998-02-10 00:00:00

abstract：:The crystal structure of an extracellular triglyceride lipase (from a fungus Rhizomucor miehei) inhibited irreversibly by diethyl p-nitrophenyl phosphate (E600) was solved by X-ray crystallographic methods and refined to a resolution of 2.65 A. The crystals are isomorphous with those of n-hexylphosphonate ethyl ester/...

journal_title：Biochemistry

authors： Derewenda U,Brzozowski AM,Lawson DM,Derewenda ZS

更新日期：1992-02-11 00:00:00

abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...

journal_title：Biochemistry

authors： Lee RT,Myers RW,Lee YC

更新日期：1982-11-23 00:00:00