A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides.

abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...

journal_title：Biochemistry

authors： Rizzo V,Stankowski S,Schwarz G

更新日期：1987-05-19 00:00:00

abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...

journal_title：Biochemistry

authors： Guillén Schlippe YV,Hedstrom L

更新日期：2005-09-06 00:00:00

abstract：:The trifunctional dinuclear platinum compounds 1,2/c,c [[cis-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) and 1,2/t,c [[trans-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) contain a monofunctional platinum coordination sphere linked to a cis-[PtCl(2)(amine)(2)] moiety. The compounds ...

journal_title：Biochemistry

authors： Kloster M,Kostrhunova H,Zaludova R,Malina J,Kasparkova J,Brabec V,Farrell N

更新日期：2004-06-22 00:00:00

abstract：:The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...

journal_title：Biochemistry

authors： Switzer ME,Gordon HJ,McKee PA

更新日期：1983-03-15 00:00:00

abstract：:A small metal-binding protein (SmbP) with no known similarity to other proteins in current databases was isolated and characterized from the periplasm of Nitrosomonas europaea. The primary structure of this small (9.9 kDa) monomeric protein is characterized by a series of 10 repeats of a seven amino acid motif and an ...

journal_title：Biochemistry

authors： Barney BM,LoBrutto R,Francisco WA

更新日期：2004-09-07 00:00:00

abstract：:In this study, we have investigated the influence of regions outside the DNA-binding domain of the human glucocorticoid receptor on high-affinity DNA binding. We find that the DNA-binding domain shows a 10-fold lower affinity for a palindromic DNA-binding site than the intact receptor. The N-terminal part of the recep...

journal_title：Biochemistry

authors： Dahlman-Wright K,Wright AP,Gustafsson JA

更新日期：1992-09-22 00:00:00

abstract：:Protein flexibility is thought to play key roles in numerous biological processes, including antibody affinity maturation, signal transduction, and enzyme catalysis, yet only limited information is available regarding the molecular details linking protein dynamics with function. A single point mutation at the distal s...

journal_title：Biochemistry

authors： Grossman M,Tworowski D,Dym O,Lee MH,Levy Y,Murphy G,Sagi I

更新日期：2010-07-27 00:00:00

abstract：:The number of self-cleaving ribozymes has increased sharply in recent years, giving rise to elaborations of the four known ribozyme catalytic strategies, α, β, γ, and δ. One such extension is utilized by the twister ribozyme, which is hypothesized to conduct δ, or general acid catalysis, via N3 of the syn adenine +1 n...

journal_title：Biochemistry

authors： Messina KJ,Kierzek R,Tracey MA,Bevilacqua PC

更新日期：2019-12-03 00:00:00

abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

journal_title：Biochemistry

authors： Carpenter KA,Schiller PW

更新日期：1998-12-01 00:00:00

abstract：:We consider the effects that different lipid surfaces have upon the denaturation and subsequent formation of amyloid fibrils of bovine insulin. The adsorption and unfolding kinetics of insulin being adsorbed onto the different lipid surfaces under denaturing conditions are studied using FTIR ATR spectroscopy and are c...

journal_title：Biochemistry

authors： Sharp JS,Forrest JA,Jones RA

更新日期：2002-12-31 00:00:00

abstract：:Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...

journal_title：Biochemistry

authors： Yarney TA,Sairam MR,Bhargavi GN,Mohapatra SK

更新日期：1990-04-17 00:00:00

abstract：:The sequential solid-phase synthesis of a peptide analogue of bovine brain calmodulin calcium binding site III covering residues 81-113 of the natural sequence is described. Methionine-109 is replaced by a leucine residue to avoid complications in the synthesis and purification. In an attempt to relate the structure o...

journal_title：Biochemistry

authors： Reid RE

更新日期：1987-09-22 00:00:00

abstract：:The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...

journal_title：Biochemistry

authors： Cane DE,Chiu HT,Liang PH,Anderson KS

更新日期：1997-07-08 00:00:00

abstract：:Increasing efforts are being invested in the construction of nanostructures with desired shapes and physical and chemical properties. Our strategy involves nanostructure design using naturally occurring protein building blocks. Inspection of the protein structural database (PDB) reveals the richness of the conformatio...

journal_title：Biochemistry

authors： Zheng J,Zanuy D,Haspel N,Tsai CJ,Alemán C,Nussinov R

更新日期：2007-02-06 00:00:00

abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

journal_title：Biochemistry

authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

更新日期：2008-01-22 00:00:00

abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...

journal_title：Biochemistry

authors： Nixon CF,Lim SA,Sailer ZR,Zheludev IN,Gee CL,Kelch BA,Harms MJ,Marqusee S

更新日期：2021-01-26 00:00:00

abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...

journal_title：Biochemistry

authors： Morita I,Smith WL,DeWitt DL,Schindler M

更新日期：1995-05-30 00:00:00

abstract：:Single-strand-specific nucleases are a diverse and important group of enzymes that are able to cleave a variety of DNA structures present in duplex molecules. Nuclease SP, an enzyme from spinach, has been purified to apparent homogeneity, allowing for the unambiguous characterization of a number of its physical proper...

journal_title：Biochemistry

authors： Strickland JA,Marzilli LG,Puckett JM Jr,Doetsch PW

更新日期：1991-10-08 00:00:00

abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

journal_title：Biochemistry

authors： Vedeckis WV

更新日期：1983-04-12 00:00:00

abstract：:Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...

journal_title：Biochemistry

authors： Bazzi MD,Nelsestuen GL

更新日期：1988-10-04 00:00:00

abstract：:Olivopontocerebellar atrophy with retinal degeneration is a hereditary neurodegenerative disorder that belongs to the subtype II of the autosomal dominant cerebellar ataxias and is characterized by early-onset cerebellar and macular degeneration preceded by diagnostically useful tritan colorblindness. The gene mutated...

journal_title：Biochemistry

authors： Mushegian AR,Vishnivetskiy SA,Gurevich VV

更新日期：2000-06-13 00:00:00

abstract：:The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...

journal_title：Biochemistry

authors： Buzón V,Padrós E,Cladera J

更新日期：2005-10-11 00:00:00

abstract：:The steady-state and pre-steady-state kinetic parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-NO(2))-Gln-Arg-NH(2) were determined in 1.25 M NaCl, 0.1 M MES-TRIS at pH 6.0 at 25 degrees C. At low concentrations of enzyme, the values of the K(m) and k(cat) calculated ...

journal_title：Biochemistry

authors： Porter DJ,Hanlon MH,Furfine ES

更新日期：2002-01-29 00:00:00

abstract：:Calcium dynamics and its linked molecular interactions cause a variety of biological responses; thus, exploiting techniques for detecting both concurrently is essential. Here we describe a method for measuring the cytosolic Ca(2+) concentration ([Ca(2+)](i)) and protein-protein interactions within the same cell, using...

journal_title：Biochemistry

authors： Mori MX,Imai Y,Itsuki K,Inoue R

更新日期：2011-05-31 00:00:00

abstract：:The direct transfer of NADH between A-B pairs of dehydrogenases and also the dissociation of NADH from individual E-NADH complexes have been investigated by transient stopped-flow kinetic techniques. Such A-B transfers of NADH occur without the intermediate dissociation of coenzyme into the aqueous solvent environment...

journal_title：Biochemistry

authors： Srivastava DK,Bernhard SA

更新日期：1987-03-10 00:00:00

abstract：:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...

journal_title：Biochemistry

authors： Zang LH,Ghosh S,Maki AH

更新日期：1989-03-07 00:00:00

abstract：:The idea of establishing the amyloid-like fibrillation tendency of pro- and antisurvival proteins of human apoptotic pathways is relevant for delineating the conditions that lead to aberrant differentiation, development, and tissue homeostasis. As the first step in this direction, we report here that the caspase recru...

journal_title：Biochemistry

authors： Rao PN,Reddy KS,Bhuyan AK

更新日期：2009-08-18 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00

abstract：:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...

journal_title：Biochemistry

authors： Di Giambattista M,Engelborghs Y,Nyssen E,Clays K,Cocito C

更新日期：1991-07-23 00:00:00

abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...

journal_title：Biochemistry

authors： Nakamura S,Seki Y,Katoh E,Kidokoro S

更新日期：2011-04-19 00:00:00