Abstract：
:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high salt concentrations was evaluated by solution X-ray scattering (SXS), dynamic light scattering, and circular dichroism measurements. MG1 was globular and slightly (3%) larger than the native state, N. Calorimetric methods, such as differential scanning calorimetry and isothermal acid-titration calorimetry, were used to evaluate the thermodynamic parameters in the transitions of N to MG1 and MG1 to denatured state D of horse cytochrome c. The heat capacity change, ΔC(p), in the N-to-MG1 transition was determined to be 2.56 kJ K(-1) mol(-1), indicating the increase in the level of hydration in the MG1 state. Moreover, the intermediate state on the thermal N-to-D transition of horse cytochrome c at pH 4 under low-salt conditions showed the same structural and thermodynamic properties of the MG1 state in both SXS and calorimetric measurements. The Gibbs free energy changes (ΔG) for the N-to-MG1 and N-to-D transitions at 15 °C were 10.9 and 42.2 kJ mol(-1), respectively.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nakamura S,Seki Y,Katoh E,Kidokoro Sdoi
10.1021/bi101806bsubject
Has Abstractpub_date
2011-04-19 00:00:00pages
3116-26issue
15eissn
0006-2960issn
1520-4995journal_volume
50pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1014503
更新日期：2010-12-07 00:00:00
abstract：:The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00596a014
更新日期：1978-02-07 00:00:00
abstract：:Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101776t
更新日期：2011-02-01 00:00:00
abstract：:The calcium-dependent binding of two synthetic 18-residue peptides derived from the calmodulin binding region of skeletal myosin light chain kinase to wild-type Drosophila melanogaster calmodulin and four calcium binding site calmodulin mutants has been investigated using optical spectroscopy. The WFF peptide (with W4...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00007a001
更新日期：1995-02-21 00:00:00
abstract：:A novel inhibitor of p38 mitogen-activated protein kinase (p38), CMPD1, identified by high-throughput screening, is characterized herein. Unlike the p38 inhibitors described previously, this inhibitor is substrate selective and noncompetitive with ATP. In steady-state kinetics experiments, CMPD1 was observed to preven...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0495073
更新日期：2004-09-21 00:00:00
abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973182r
更新日期：1998-06-30 00:00:00
abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035018b
更新日期：2003-10-14 00:00:00
abstract：:αB-Crystallin is a chaperone and an anti-apoptotic protein that is strongly expressed in many tissues, including the lens, retina, heart, and kidney. In the human lens, several lysine residues in αB-crystallin are acetylated. We have previously shown that such acetylation is predominant at lysine 92 (K92) and lysine 1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400638s
更新日期：2013-11-12 00:00:00
abstract：:Gramicidin crystals, dimyristoylphosphatidylcholine (DMPC)/gramicidin dispersions, and DMPC/gramicidin cocrystals were examined by Raman scattering to determine lipid/gramicidin stoichiometries and lipid organization. Calibrations of the choline (716-cm-1) and tryptophan (756-cm-1) peaks indicate that the cocrystals c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00376a030
更新日期：1987-01-27 00:00:00
abstract：:The single-subunit RNA polymerases make up a widespread family of proteins found in phage, mitochondria, and chloroplasts. Unlike the phage RNAPs, the eukaryotic RNAPs require accessory factors to melt their promoters and diverge from the phage RNAPs in the regions where functions associated with promoter melting in t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300074j
更新日期：2012-05-08 00:00:00
abstract：:Proton-detected heteronuclear multiple-bond 1H-13C correlations (HMBC) previously have been used for assignment purposes in a variety of isotopically enriched proteins. In the present study it is demonstrated that the technique yields an almost complete assignment of the natural abundance 13C spectrum of the protein b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00107a014
更新日期：1991-10-29 00:00:00
abstract：:Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9928086
更新日期：2000-04-11 00:00:00
abstract：:The protein phosphatase 2C (PP2C) family represents one of the four major protein Ser/Thr phosphatase activities in mammalian cells and contains at least 13 distinct gene products. Although PP2C family members regulate a variety of cellular functions, mechanisms of regulation of their activities are largely unknown. H...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800067p
更新日期：2008-07-08 00:00:00
abstract：:The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00309a021
更新日期：1984-07-03 00:00:00
abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9521351
更新日期：1996-02-27 00:00:00
abstract：:Several mutant Escherichia coli aspartate aminotransferases (eAATases) have been characterized in the attempt to evolve or rationally redesign the substrate specificity of eAATase into that of E. coli tyrosine aminotransferase (eTATase). These include HEX (designed), HEX + A293D (design followed by directed evolution)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0487544
更新日期：2004-10-12 00:00:00
abstract：:The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963018o
更新日期：1997-07-08 00:00:00
abstract：:A rapid assay procedure was developed for cleavage of the N-terminal propeptides of procollagen. With the assay a neutral procollagen N-protease was purified about 300-fold from chick embryo tendon extract. The enzyme had an apparent molecular weight of 260 000 and a pH optimum of 7.4. Ca2+ was required for enzymic ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00608a002
更新日期：1978-07-25 00:00:00
abstract：:13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00298a017
更新日期：1984-01-31 00:00:00
abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981072u
更新日期：1998-11-10 00:00:00
abstract：:A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0001675
更新日期：2000-05-23 00:00:00
abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802298r
更新日期：2009-02-24 00:00:00
abstract：:Streptomyces avermitilis, an industrial organism responsible for the production of the anthelminthic avermectins, harbors a 13.4 kb gene cluster containing 13 unidirectionally transcribed open reading frames corresponding to the apparent biosynthetic operon for the sesquiterpene antibiotic pentalenolactone. The advanc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060419n
更新日期：2006-05-16 00:00:00
abstract：:Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00350a016
更新日期：1986-01-28 00:00:00
abstract：:Low molecular weight protein tyrosine phosphatases (LMW-PTPs) are small enzymes that are ubiquitous in many organisms. They are important in biological processes such as cell proliferation, adhesion, migration, and invasiveness. LMW-PTP is expressed in mammalian cells as two isoforms (IF1 and IF2) originating through ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0620858
更新日期：2007-05-29 00:00:00
abstract：:The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011626k
更新日期：2002-02-12 00:00:00
abstract：:We have used flash-detection optical and electron paramagnetic resonance spectroscopy to measure the kinetics and yield per flash of the photooxidation of cytochrome b559 and the yield per flash of the photooxidation of the tyrosine residue YD in Mn-depleted photosystem II (PSII) membranes at room temperature. The ini...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00490a014
更新日期：1990-09-25 00:00:00
abstract：:The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00287a013
更新日期：1983-08-30 00:00:00
abstract：:In a previously determined minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment (KF) of Escherichia coli DNA polymerase I, a nonchemical step that interconverted the KF'.DNAn+1.PPi and KF.DNAn+1PPi complexes was not observed in correct incorporation [Kuchta, R. D., Mizrahi, V., Benkovic, P.A....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00234a002
更新日期：1991-05-21 00:00:00
abstract：:Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are examined. Initial s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991019l
更新日期：1999-09-14 00:00:00
