Abstract:
:The calcium-dependent binding of two synthetic 18-residue peptides derived from the calmodulin binding region of skeletal myosin light chain kinase to wild-type Drosophila melanogaster calmodulin and four calcium binding site calmodulin mutants has been investigated using optical spectroscopy. The WFF peptide (with W4 and F17) and the FFW peptide (with F4 and W17) both bind to wild-type calmodulin with 1:1 stoichiometry and Kd values of < or = 0.2 and 1.6 nM, respectively. Near-UV CD spectra of the protein-peptide complexes suggest that both peptides bind in the same orientation, with the side chain of residue 4 interacting with the C-domain of calmodulin and that of residue 17 with the N-domain [as in the structure of the calmodulin-M13 peptide complex determined by Ikura et al. [Ikura, M., Clore, G. M., Gronenborn, A. M., Zhu, G., Klee, C. B., & Bax, A. (1992) Science 256, 632-638]]. Both peptides have lower affinities for all the mutant calmodulins than for the wild-type protein. Fluorescence measurements suggest that mutation of calcium binding site 2 in the N-domain does not affect the interaction of the W4 side chain of the WFF peptide with the C-domain of calmodulin. However, the E67Q (B2Q) but not the E67K (B2K) mutation (site 2, N-domain) alters the interaction of W17 of the FFW peptide with the protein. In contrast, the E140K (B4K) mutation has a much greater effect than the E140Q (B4Q) mutation (site 4, C-domain) on the interaction of calmodulin with both peptides.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Findlay WA,Martin SR,Beckingham K,Bayley PMdoi
10.1021/bi00007a001subject
Has Abstractpub_date
1995-02-21 00:00:00pages
2087-94issue
7eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::To probe the structure and stability of the central region of lipid-free apolipoprotein (apo) A-I (residues 123-165), we studied the effects of four mutations made in this region on the conformation, stability, dimyristoylphosphatidylcholine (DMPC) binding kinetics, and size of discoidal reconstituted high-density lip...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025807d
更新日期:2002-08-20 00:00:00
abstract::The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00249a009
更新日期:1994-11-15 00:00:00
abstract::By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a020
更新日期:1988-05-31 00:00:00
abstract::Indolethylamine-N-methyltransferase (INMT) is a Class 1 transmethylation enzyme known for its production of N,N-dimethyltryptamine (DMT), a hallucinogen with affinity for various serotonergic, adrenergic, histaminergic, dopaminergic, and sigma-1 receptors. DMT is produced via the action of INMT on the endogenous subst...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500175p
更新日期:2014-05-13 00:00:00
abstract::Ankyrin repeat (AR) proteins possess a distinctive modular and repetitive architecture, and their global folds are maintained by short-range interactions in terms of the primary sequence. In this work, we extended our previous study on the highly conserved TPLH tetrapeptide and investigated the impact of a solvent-exp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005717
更新日期:2013-07-16 00:00:00
abstract::While within a human host the opportunistic pathogen Toxoplasma gondii relies heavily on glycolysis for its energy needs. Lactate dehydrogenase (LDH), the terminal enzyme in anaerobic glycolysis necessary for NAD(+) regeneration, therefore represents an attractive therapeutic target. The tachyzoite stage lactate dehyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035108g
更新日期:2004-02-03 00:00:00
abstract::Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is als...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.9b00164
更新日期:2019-12-31 00:00:00
abstract::The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0503798
更新日期:2005-06-14 00:00:00
abstract::P16(INK4A) (also known as P16 and MTS1), a protein consisting exclusively of four ankyrin repeats, is recognized as a tumor suppressor mainly because of the prevalence of genetic inactivation of the p16(INK4A) (or CDKN2A) gene in virtually all types of human cancers. However, it has also been shown that an elevated le...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi200642e
更新日期:2011-06-28 00:00:00
abstract::A compound tentatively identified as O2-methyl-5-carboxymethyluridine (cm5Um) was recently isolated in this laboratory from bulk yeast transfer RNA (Gray, M. W. (1975), Can, J. Biochem. 53, 735-746). Alkaline hydrolysis of yeast tRNA releases this nucleoside as part of an alkali-stable dinucleotide, cm5Um-Ap, from whi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00659a017
更新日期:1976-07-13 00:00:00
abstract::We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00422
更新日期:2019-11-26 00:00:00
abstract::The cyanogen bromide fragment comprising residues 115-181 of Kunitz soybean trypsin inhibitor is a soluble random-coil peptide at pH 7 containing two cysteines separated by eight other amino acids in the primary sequence. Four of the six rate constants have been determined for the three disulfide exchange reactions be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00377a005
更新日期:1987-02-10 00:00:00
abstract::Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a011
更新日期:1995-04-11 00:00:00
abstract::The mechanism of oxidation of a peptide substrate by the flavoprotein lysine-specific demethylase (LSD1) has been examined using the effects of pH and isotopic substitution on steady-state and rapid-reaction kinetic parameters. The substrate contained the 21 N-terminal residues of histone H3, with a dimethylated lysyl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900499w
更新日期:2009-06-16 00:00:00
abstract::The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00272a026
更新日期:1983-02-01 00:00:00
abstract::Bacterial L-asparaginases, enzymes that catalyze the hydrolysis of L-asparagine to aspartic acid, have been used for over 30 years as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. Other substrates of asparaginases include L-glutamine, D-asparagine, and succinic acid monoamide. In this ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0029595
更新日期:2001-05-15 00:00:00
abstract::The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030065p
更新日期:2003-07-29 00:00:00
abstract::The human endonuclease III homologue (hNTH1) removes premutagenic cytosine damage from DNA. This includes 5-hydroxycytosine, which has increased potential for pairing with adenine, resulting in C --> T transition mutations. Here we report that hNTH1 acts on both 5-hydroxycytosine and abasic sites preferentially when t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0028901
更新日期:2001-06-05 00:00:00
abstract::Peptide nucleic acids (PNAs) are nonionic DNA/RNA mimics that can recognize complementary sequences by Watson-Crick base pairing. The neutral PNA backbone facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs) can be important tools for exploring the structure and function...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700230a
更新日期:2007-06-26 00:00:00
abstract::Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2003923
更新日期:2011-06-28 00:00:00
abstract::The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00141a029
更新日期:1992-07-07 00:00:00
abstract::A new strategy to form stable and sequence-specific triple helical DNA structures at mixed purine/pyrimidine sequences using a combination of four C-glycosides (TRIPsides) has been described [Li et al. (2003) J. Am. Chem. Soc. 125, 2084]. The partial realization of the approach is demonstrated by incorporating two of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035648d
更新日期:2004-02-17 00:00:00
abstract::The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962885q
更新日期:1997-06-10 00:00:00
abstract::DNA topoisomerase II is believed to be the enzyme that produces the protein-associated DNA strand breaks observed in mammalian cell nuclei treated with various intercalating agents. Two intercalators--4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA, amsacrine) and 2-methyl-9-hydroxyellipticinium (2-Me-9-OH-E+)-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00344a015
更新日期:1985-11-05 00:00:00
abstract::Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00251a012
更新日期:1994-11-29 00:00:00
abstract::We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00251a030
更新日期:1994-11-29 00:00:00
abstract::The vitamin D receptor (VDR) binds 1,25-dihydroxyvitamin D(3) and mediates its actions on gene transcription by heterodimerizing with retinoid X receptors (RXRs) on direct repeat (DR+3) vitamin D responsive elements (VDREs) located in target genes. The VDRE binding function of VDR has been primarily ascribed to the zi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9916574
更新日期:1999-12-07 00:00:00
abstract::The SecA molecular nanomachine in bacteria uses energy from ATP hydrolysis to drive post-translational secretion of preproteins through the SecYEG translocon. Cytosolic SecA exists in a dimeric, "closed" state with relatively low ATPase activity. After binding to the translocon, SecA undergoes major conformational rea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201793e
更新日期:2012-02-21 00:00:00
abstract::The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00277a042
更新日期:1983-04-12 00:00:00
abstract::Peptide deformylase (EC 3.5.1.31) catalyzes the removal of a formyl group from the N-termini of nascent ribosome-synthesized polypeptides, an obligatory step during protein maturation in eubacteria. Since its discovery in crude Escherichia coli extracts 3 decades ago, the deformylase has resisted all attempts of purif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971155v
更新日期:1997-11-11 00:00:00