Location of an alpha-helix in fragment 96-133 from bovine somatotropin by 1H NMR spectroscopy.

abstract：:Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...

journal_title：Biochemistry

authors： Li J,Shinjo M,Matsumura Y,Morita M,Baker D,Ikeguchi M,Kihara H

更新日期：2007-05-01 00:00:00

abstract：:We previously reported that the microtubule (MT)-binding domain of microtubule-associated protein 4 (MAP4) contains three 18-amino acid imperfect repeats that are homologous to the repeats found in the MT-binding domains of the neuronal MAPs, MAP2 and tau [Chapin, S. J., & Bulinski, J. C. (1991) J. Cell Sci. 98, 27-36...

journal_title：Biochemistry

authors： Chapin SJ,Lue CM,Yu MT,Bulinski JC

更新日期：1995-02-21 00:00:00

abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

journal_title：Biochemistry

authors： Mizzer JP,Thorpe C

更新日期：1981-08-18 00:00:00

abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

journal_title：Biochemistry

authors： Holowka DA,Cathou RE

更新日期：1976-07-27 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...

journal_title：Biochemistry

authors： Merkle D,Block WD,Yu Y,Lees-Miller SP,Cramb DT

更新日期：2006-04-04 00:00:00

abstract：:The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...

journal_title：Biochemistry

authors： Bures EJ,Hui JO,Young Y,Chow DT,Katta V,Rohde MF,Zeni L,Rosenfeld RD,Stark KL,Haniu M

更新日期：1998-09-01 00:00:00

abstract：:D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...

journal_title：Biochemistry

authors： Toteva MM,Silvaggi NR,Allen KN,Richard JP

更新日期：2011-11-22 00:00:00

abstract：:Gap junctions, transmembrane protein channels that directly connect the cytoplasm of neighboring cells and enable the exchange of molecules between cells, are a promising new frontier for therapeutic delivery. Specifically, cell-derived lipid vesicles that contain functional gap junction channels, termed Connectosomes...

journal_title：Biochemistry

authors： Gadok AK,Zhao C,Meriwether AI,Ferrati S,Rowley TG,Zoldan J,Smyth HDC,Stachowiak JC

更新日期：2018-01-09 00:00:00

abstract：:Over 155 mutations within the V2 vasopressin receptor (AVPR2) gene are responsible for nephrogenic diabetes insipidus (NDI). The expression and subcellular distribution of four of these was investigated in transfected cells. These include a point mutation in the seventh transmembrane domain (S315R), a frameshift mutat...

journal_title：Biochemistry

authors： Morello JP,Salahpour A,Petäjä-Repo UE,Laperrière A,Lonergan M,Arthus MF,Nabi IR,Bichet DG,Bouvier M

更新日期：2001-06-12 00:00:00

abstract：:The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...

journal_title：Biochemistry

authors： Huang HW

更新日期：2000-07-25 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:Oocytes released en masse from pig ovaries were isolated in large quantities by using sieving techniques. The isolated oocytes were gently homogenized, and the largely intact zona pellucida "ghosts" were purified by using sieving techniques. Sufficient amounts of zonae were recovered to permit, for the first time, ade...

journal_title：Biochemistry

authors： Dunbar BS,Wardrip NJ,Hedrick JL

更新日期：1980-01-22 00:00:00

abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...

journal_title：Biochemistry

authors： Seffernick JL,McTavish H,Osborne JP,de Souza ML,Sadowsky MJ,Wackett LP

更新日期：2002-12-03 00:00:00

abstract：:15N NMR spectroscopy was used to examine the active-site histidyl residue of alpha-lytic protease in peptide boronic acid inhibitor complexes. Two distinct types of complexes were observed: (1) Boronic acids that are analogues of substrates form complexes in which the active-site imidazole ring is protonated and both ...

journal_title：Biochemistry

authors： Bachovchin WW,Wong WY,Farr-Jones S,Shenvi AB,Kettner CA

更新日期：1988-10-04 00:00:00

abstract：:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...

journal_title：Biochemistry

authors： Koch N,Hämmerling GJ

更新日期：1985-10-22 00:00:00

abstract：:Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...

journal_title：Biochemistry

authors： Hübner G,König S,Koch MH,Hengstenberg W

更新日期：1995-12-05 00:00:00

abstract：:Bacterial bioluminescence is a phenomenon resulting from the reaction of a two-component FMN-dependent aldehyde monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. Bacterial luciferase (LuxAB) is one of the most extensively investigated two-component monooxygenases, while its reductase...

journal_title：Biochemistry

authors： Nijvipakul S,Ballou DP,Chaiyen P

更新日期：2010-11-02 00:00:00

abstract：:The three-dimensional structure of the complex between calmodulin (CaM) and a peptide corresponding to the N-terminal portion of the CaM-binding domain of the plasma membrane calcium pump, the peptide C20W, has been solved by heteronuclear three-dimensional nuclear magnetic resonance (NMR) spectroscopy. The structure ...

journal_title：Biochemistry

authors： Elshorst B,Hennig M,Försterling H,Diener A,Maurer M,Schulte P,Schwalbe H,Griesinger C,Krebs J,Schmid H,Vorherr T,Carafoli E

更新日期：1999-09-21 00:00:00

abstract：:Escherichia coli mannitol specific EII in membrane vesicles can be inhibited by the action of the oxidizable substrate-reduced phenazine methosulfate (PMS) in a manner similar to E. coli enzyme IIGlc [Robillard, G. T., & Konings, W. (1981) Biochemistry 20, 5025-5032]. The fact that reduced PMS and various oxidizing ag...

journal_title：Biochemistry

authors： Roossien FF,Robillard GT

更新日期：1984-01-17 00:00:00

abstract：:Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...

journal_title：Biochemistry

authors： Oertell K,Chamberlain BT,Wu Y,Ferri E,Kashemirov BA,Beard WA,Wilson SH,McKenna CE,Goodman MF

更新日期：2014-03-25 00:00:00

abstract：:We have employed molecular dynamics (MD) simulation to investigate, with atomic details, the structural dynamics and energetics of three major ATPase states (ADP, APO, and ATP state) of a human kinesin-1 monomer in complex with a tubulin dimer. Starting from a recently solved crystal structure of ATP-like kinesin-tubu...

journal_title：Biochemistry

authors： Chakraborty S,Zheng W

更新日期：2015-01-27 00:00:00

abstract：:Excluded volume and viscosity effects of crowding agents that mimic crowded conditions in vivo on "classical" burst phase folding kinetics of cytochrome c are assessed in vitro. Upon electron transfer-triggered folding of reduced cytochrome c, far-UV time-resolved circular dichroism (TRCD) is used to monitor folding u...

journal_title：Biochemistry

authors： Chen E,Christiansen A,Wang Q,Cheung MS,Kliger DS,Wittung-Stafshede P

更新日期：2012-12-11 00:00:00

abstract：:The redox properties of the [2Fe-2S] cluster in the 24 kDa subunit of bovine heart mitochondrial NADH:ubiquinone oxidoreductase (complex I) and three of its homologues have been defined using protein-film voltammetry. The clusters in all four examples display characteristic, pH-dependent redox transitions, which, unus...

journal_title：Biochemistry

authors： Zu Y,Di Bernardo S,Yagi T,Hirst J

更新日期：2002-08-06 00:00:00

abstract：:S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. As dimer formation is crucial to S100 function, we applied a yeast two-hybrid...

journal_title：Biochemistry

authors： Koltzscher M,Gerke V

更新日期：2000-08-08 00:00:00

abstract：:Fragment-based approaches to finding novel small molecules that bind to proteins are now firmly established in drug discovery and chemical biology. Initially developed primarily in a few centers in the biotech and pharma industry, this methodology has now been adopted widely in both the pharmaceutical industry and aca...

journal_title：Biochemistry

authors： Scott DE,Coyne AG,Hudson SA,Abell C

更新日期：2012-06-26 00:00:00

abstract：:The metalloregulatory protein MntR from Bacillus subtilis acts as a transcriptional regulator of manganese homeostasis. MntR is a member of a subfamily of DtxR-related proteins that perform analogous regulatory functions in a variety of pathogenic organisms. Metal ions activate MntR to bind DNA and repress the transcr...

journal_title：Biochemistry

authors： Lieser SA,Davis TC,Helmann JD,Cohen SM

更新日期：2003-11-04 00:00:00

abstract：:NahE and PhdJ are bifunctional hydratase-aldolases in bacterial catabolic pathways for naphthalene and phenanthrene, respectively. Bacterial species with these pathways can use polycyclic aromatic hydrocarbons (PAHs) as sole sources of carbon and energy. Because of the harmful properties of PAHs and their widespread d...

journal_title：Biochemistry

authors： LeVieux JA,Medellin B,Johnson WH Jr,Erwin K,Li W,Johnson IA,Zhang YJ,Whitman CP

更新日期：2018-06-26 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

journal_title：Biochemistry

authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

更新日期：2005-02-15 00:00:00

abstract：:Receptor tyrosine kinase (RTK) activation is associated with modulation of heptahelical receptor-stimulated adenylyl cyclase responses. The mechanisms underlying the RTK-mediated enhancement of adenylyl cyclase function remain unclear. In the present studies, we show that the tyrosine kinase-dependent enhancement of a...

journal_title：Biochemistry

authors： Tan CM,Kelvin DJ,Litchfield DW,Ferguson SS,Feldman RD

更新日期：2001-02-13 00:00:00

abstract：:The ATP analogue 5'-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA) was used to chemically modify the ATP binding sites of the carbamyl phosphate synthetase domain of CAD, the multifunctional protein that catalyzes the first steps in mammalian pyrimidine biosynthesis. Reaction of CAD with FSBA resulted in the inactivation...

journal_title：Biochemistry

authors： Kim HS,Lee L,Evans DR

更新日期：1991-10-22 00:00:00