Influence of phosphoenolpyruvate and magnesium ions on the quaternary structure of enzyme I of the phosphotransferase system from gram-positive bacteria.

abstract：:Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...

journal_title：Biochemistry

authors： Huster D,Xiao L,Hong M

更新日期：2001-06-26 00:00:00

abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

journal_title：Biochemistry

authors： Richardson CJ,First EA

更新日期：2016-03-15 00:00:00

abstract：:The calcitonin gene-related peptide (CGRP) receptor is a heterodimer of two membrane proteins: calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). CLR is a class B G-protein-coupled receptor (GPCR), possessing a characteristic large amino-terminal extracellular domain (ECD) impor...

journal_title：Biochemistry

authors： Koth CM,Abdul-Manan N,Lepre CA,Connolly PJ,Yoo S,Mohanty AK,Lippke JA,Zwahlen J,Coll JT,Doran JD,Garcia-Guzman M,Moore JM

更新日期：2010-03-09 00:00:00

abstract：:The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...

journal_title：Biochemistry

authors： Chen P,Evans CL,Hirst JD,Searle MS

更新日期：2011-01-11 00:00:00

abstract：:HK31 (S565-K595) has previously been shown to encompass the binding domain for plasma prekallikrein (PK) within domain 6 of high molecular weight kininogen (HK). The complementary binding domain for HK within PK is mapped to PK56 (F56-G86), in the apple 1 domain, and to PK266 (K266-C295), in the apple 4 domain. Isothe...

journal_title：Biochemistry

authors： Lin Y,Shenoy SS,Harris RB,Colman RW

更新日期：1996-10-01 00:00:00

abstract：:The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...

journal_title：Biochemistry

authors： Tong X,Zhu J,Ma Y,Chen X,Wu G,He F,Cao C,Wu H

更新日期：2007-10-09 00:00:00

abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...

journal_title：Biochemistry

authors： Du D,Ju H,Zhang X,Chen J,Cai J,Chen H

更新日期：2005-08-30 00:00:00

abstract：:In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...

journal_title：Biochemistry

authors： King L,Liu CC,Lee RF

更新日期：1994-05-10 00:00:00

abstract：:We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I) molecules on the cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crysta...

journal_title：Biochemistry

authors： Sun X,Tokunaga R,Nagai Y,Miyahara R,Kishimura A,Kawakami S,Katayama Y,Mori T

更新日期：2020-12-15 00:00:00

abstract：:Ultraviolet resonance Raman (UV RR) spectra are reported for ferricytochrome c from tuna and horse heart at pH 1.6, 7, 10, and 13, representing distinct conformational states of the protein (states II, III, IV, and V, respectively). The spectra were obtained with pulsed laser excitation at 200 and 218 nm, via H2 Raman...

journal_title：Biochemistry

authors： Copeland RA,Spiro TG

更新日期：1985-08-27 00:00:00

abstract：:Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge...

journal_title：Biochemistry

authors： Connell GJ,Illangesekare M,Yarus M

更新日期：1993-06-01 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:The occurrence of isozymes in plant peroxidases is poorly understood. Turnip roots contain seven season-dependent isoperoxidases with distinct physicochemical properties. In the work presented here, multifrequency electron paramagnetic resonance spectroscopy has been used to characterize the Compound I intermediate ob...

journal_title：Biochemistry

authors： Ivancich A,Mazza G,Desbois A

更新日期：2001-06-12 00:00:00

abstract：:CNAD (5-beta-D-ribofuranosylnicotinamide adenine dinucleotide) is an isosteric C-glycosidic analogue of NAD(H) containing a neutral pyridine ring. CPAD (5-beta-D-ribofuranosylpicolinamide adenine dinucleotide) is a closely related pyridine-containing analogue with the pyridine nitrogen on the opposite side of the ring...

journal_title：Biochemistry

authors： Li H,Hallows WH,Punzi JS,Pankiewicz KW,Watanabe KA,Goldstein BM

更新日期：1994-10-04 00:00:00

abstract：:We have combined equilibrium and steered molecular dynamics (SMD) simulations with principal component and correlation analyses to probe the mechanism of allosteric regulation in imidazole glycerol phosphate (IGP) synthase. An evolutionary analysis of IGP synthase revealed a conserved network of interactions leading f...

journal_title：Biochemistry

authors： Amaro RE,Sethi A,Myers RS,Davisson VJ,Luthey-Schulten ZA

更新日期：2007-02-27 00:00:00

abstract：:The unfolding and refolding properties of human lysozyme and two amyloidogenic variants (Ile56Thr and Asp67His) have been studied by stopped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry. The unfolding of each protein in 5.4 M guanidine hydrochloride (GuHCl) is well described as...

journal_title：Biochemistry

authors： Canet D,Sunde M,Last AM,Miranker A,Spencer A,Robinson CV,Dobson CM

更新日期：1999-05-18 00:00:00

abstract：:S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...

journal_title：Biochemistry

authors： Katusz RM,Colman RF

更新日期：1991-11-26 00:00:00

abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

journal_title：Biochemistry

authors： Tripet BP,Goel A,Copie V

更新日期：2011-06-14 00:00:00

abstract：:Vaccinia DNA topoisomerase, a 314 amino acid type I enzyme, catalyzes the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward arrow. To identify amino acids that participate in the DNA binding and transesterification st...

journal_title：Biochemistry

authors： Wang LK,Wittschieben J,Shuman S

更新日期：1997-07-01 00:00:00

abstract：:Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family. In this paper, for the first time, the fidelity of a Y-family polymerase, Dpo4, was determined using pre-steady-state kinetic analysis of the incorporation of a single nucle...

journal_title：Biochemistry

authors： Fiala KA,Suo Z

更新日期：2004-02-24 00:00:00

abstract：:D(--)-beta-Hydroxybutyrate dehydrogenase of beef heart mitochondria is inhibited by modifiers of disulfides, thiols, and vicinal dithiols [Phelps, D. C., & Hatefi, Y. (1981) Biochemistry 20 (preceding paper in this issue)]. The vicinal dithiol can be reversibly oxidized by diamide, resulting in activity inhibition, an...

journal_title：Biochemistry

authors： Phelps DC,Hatefi Y

更新日期：1981-02-03 00:00:00

abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...

journal_title：Biochemistry

authors： Ollagnier-De-Choudens S,Mulliez E,Hewitson KS,Fontecave M

更新日期：2002-07-23 00:00:00

abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...

journal_title：Biochemistry

authors： Pap EH,Hanicak A,van Hoek A,Wirtz KW,Visser AJ

更新日期：1995-07-18 00:00:00

abstract：:We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue mi...

journal_title：Biochemistry

authors： Shyjan AW,Levenson R

更新日期：1989-05-30 00:00:00

abstract：:The reactions of Rhodopseudomonas viridis cytochrome c2 and horse cytochrome c with Rps. viridis photosynthetic reaction centers were studied by using both single- and double-flash excitation. Single-flash excitation of the reaction centers resulted in rapid photooxidation of cytochrome c-556 in the cytochrome subunit...

journal_title：Biochemistry

authors： Knaff DB,Willie A,Long JE,Kriauciunas A,Durham B,Millett F

更新日期：1991-02-05 00:00:00

abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...

journal_title：Biochemistry

authors： Pina JM,Reynaga JM,Truong AAM,Keppetipola NM

更新日期：2018-07-03 00:00:00

abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...

journal_title：Biochemistry

authors： Bailly C,Tardy C,Wang L,Armitage B,Hopkins K,Kumar A,Schuster GB,Boykin DW,Wilson WD

更新日期：2001-08-21 00:00:00

abstract：:Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...

journal_title：Biochemistry

authors： van der Kamp MW,Sirirak J,Żurek J,Allemann RK,Mulholland AJ

更新日期：2013-11-12 00:00:00

abstract：:The 5'-untranslated region of positive-strand RNA viruses harbors many cis-acting RNA structural elements that are important for various viral processes such as replication, translation, and packaging of new virions. Among these is loop B RNA of the stem-loop IV domain within the internal ribosomal entry site (IRES) o...

journal_title：Biochemistry

authors： Du Z,Ulyanov NB,Yu J,Andino R,James TL

更新日期：2004-05-18 00:00:00