Structural insights into the two sequential folding transition states of the PB1 domain of NBR1 from Φ value analysis and biased molecular dynamics simulations.

abstract：:Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mon...

journal_title：Biochemistry

authors： Terlesky KC,Tabita FR

更新日期：1991-08-20 00:00:00

abstract：:A new strategy for the assignment of nonexchangeable proton resonances in oligonucleotide duplexes is presented and used to interpret the spectra of the oligonucleotide helix d(CpGpCpGpCpG) X d(CpGpCpGpCpG) in low salt (B form). This procedure is based on the use of sequential homodecoupling (1D) or COSY (2D) for the ...

journal_title：Biochemistry

authors： Frechet D,Cheng DM,Kan LS,Ts'o PO

更新日期：1983-10-25 00:00:00

abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...

journal_title：Biochemistry

authors： Green KD,Biswas T,Pang AH,Willby MJ,Reed MS,Stuchlik O,Pohl J,Posey JE,Tsodikov OV,Garneau-Tsodikova S

更新日期：2018-02-06 00:00:00

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00

abstract：:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

journal_title：Biochemistry

authors： Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

更新日期：2007-12-25 00:00:00

abstract：:Two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (RubisCO) enzymes were constructed using RubisCO small subunit genes (rbcS) from two eucaryotic marine organisms, Cylindrotheca sp. N1 and Olisthodiscus luteus, cloned downstream of the RubisCO large subunit gene (rbcL) of the cyanobacterium Synechococcus PCC ...

journal_title：Biochemistry

authors： Read BA,Tabita FR

更新日期：1992-06-23 00:00:00

abstract：:Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...

journal_title：Biochemistry

authors： Ueng YF,Kuwabara T,Chun YJ,Guengerich FP

更新日期：1997-01-14 00:00:00

abstract：:The alpha-helix formed by the amino acid residues 9-19 of the B-chain of insulin is involved in the stabilization of its three-dimensional structure. We have shown that modification at positions B9, B10, B12, and B16 results in analogues possessing biological activities ranging from ca. 0.2% to ca. 500% relative to th...

journal_title：Biochemistry

authors： Hu SQ,Burke GT,Schwartz GP,Ferderigos N,Ross JB,Katsoyannis PG

更新日期：1993-03-16 00:00:00

abstract：:We report the first experimental characterization of the full thermodynamic profile for binding of urea to a native protein. We measured the volumetric parameters of lysozyme at pH 7.0 as a function of urea within a temperature range of 18-45 °C. At neutral pH, lysozyme retains its native conformation between 0 and 8 ...

journal_title：Biochemistry

authors： Son I,Chalikian TV

更新日期：2016-11-29 00:00:00

abstract：:The energy derived from optimized van der Waals interactions in closely packed, folded proteins has been proposed to be of similar energetic magnitude to hydrophobicity in stabilizing the native state. If packing is this energetically important, it should influence the evolution of protein core sequences. To test this...

journal_title：Biochemistry

authors： Chen J,Stites WE

更新日期：2001-12-18 00:00:00

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:In spite of a nuclear targeting sequence, a substantial amount of dual-specificity tyrosine phosphorylation-regulated kinase (Dyrk1A) is located within the cytoplasm of neurons. Analysis of fractionated rat brains revealed that the majority of Dyrk1A was in the postnuclear precipitate. The kinase in this fraction was ...

journal_title：Biochemistry

authors： Murakami N,Bolton D,Hwang YW

更新日期：2009-10-06 00:00:00

abstract：:The solution structure of the complementary d(C1-A2-T3-G4-X5-G6-T7-A8-C9).d(G10-T11-A12-C13-A14-C15-A16-T17-G18) DNA duplex (designated X.A 9-mer), which contains a 1,N2-propanodeoxyguanosine exocyclic adduct X5 opposite deoxyadenosine A14 at the center, is pH dependent [Kouchakdjian, M., Eisenberg, M., Live, D., Mari...

journal_title：Biochemistry

authors： Huang P,Patel DJ,Eisenberg M

更新日期：1993-04-20 00:00:00

abstract：:Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...

journal_title：Biochemistry

authors： Serrano S,Huarte N,Rujas E,Andreu D,Nieva JL,Jiménez MA

更新日期：2017-10-17 00:00:00

abstract：:Homoserine transacetylase catalyzes one of the required steps in the biosynthesis of methionine in fungi and several bacteria. We have determined the crystal structure of homoserine transacetylase from Haemophilus influenzae to a resolution of 1.65 A. The structure identifies this enzyme to be a member of the alpha/be...

journal_title：Biochemistry

authors： Mirza IA,Nazi I,Korczynska M,Wright GD,Berghuis AM

更新日期：2005-12-06 00:00:00

abstract：:12-Iodo-cis-9-octadecenoic acid (12-IODE) is a time-dependent, irreversible inactivator of soybean lipoxygenase 1. The rate of inactivation is independent of 12-IODE concentration above 20 microM and is half-maximal at about 4 microM. Inactivation by 12-IODE requires lipid hydroperoxide, which must be present even aft...

journal_title：Biochemistry

authors： Rotenberg SA,Grandizio AM,Selzer AT,Clapp CH

更新日期：1988-11-29 00:00:00

abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

journal_title：Biochemistry

authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

更新日期：1987-09-08 00:00:00

abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

journal_title：Biochemistry

authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

更新日期：2011-10-11 00:00:00

abstract：:While the iron-molybdenum cofactor (FeMoco) of nitrogenase, a constituent of the active site for nitrogen reduction, can be extracted into N-methylformamide (NMF) and pyrrollidinone, the inability to solubilize it in any other organic solvents has hampered further understanding of its structure and chemical properties...

journal_title：Biochemistry

authors： Wink DA,McLean PA,Hickman AB,Orme-Johnson WH

更新日期：1989-11-28 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00

abstract：:TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded ...

journal_title：Biochemistry

authors： Vincze O,Tökési N,Oláh J,Hlavanda E,Zotter A,Horváth I,Lehotzky A,Tirián L,Medzihradszky KF,Kovács J,Orosz F,Ovádi J

更新日期：2006-11-21 00:00:00

abstract：:Solvent kinetic isotope effect studies of electron transfer within xanthine oxidase have been performed, using a stopped-flow pH-jump technique to perturb the distribution of reducing equivalents within partially reduced enzyme and follow the kinetics of reequilibration spectrophotometrically. It is found that the rat...

journal_title：Biochemistry

authors： Hille R

更新日期：1991-09-03 00:00:00

abstract：:Free cholesterol is very efficiently removed from cells by 2-hydroxypropyl-beta-cyclodextrins. The efflux of cholesterol occurs from two distinct kinetic pools: the half-times (t(1/2)) for the two pools in CHO-K1 cells are 15 +/- 5 s and 21 +/- 6 min and they represent 25% +/- 5% and 75% +/- 5% of the readily exchange...

journal_title：Biochemistry

authors： Haynes MP,Phillips MC,Rothblat GH

更新日期：2000-04-18 00:00:00

abstract：:Collagen is a multifunctional protein, serving as a structural scaffold and a modulator of cellular responses. Prior work has identified distinct regions from several collagen types that promote cell adhesion, spreading, migration, and signal transduction. One of these regions, alpha1(IV)1263-1277 from type IV collage...

journal_title：Biochemistry

authors： Malkar NB,Lauer-Fields JL,Borgia JA,Fields GB

更新日期：2002-05-14 00:00:00

abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

journal_title：Biochemistry

authors： Zein N,Solomon W,Colson KL,Schroeder DR

更新日期：1995-09-12 00:00:00

abstract：:Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...

journal_title：Biochemistry

authors： Levison SA,Hicks AN,Portmann AJ,Dandliker WB

更新日期：1975-08-26 00:00:00

abstract：:Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...

journal_title：Biochemistry

authors： Cheffer A,Ulrich H

更新日期：2011-03-22 00:00:00

abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...

journal_title：Biochemistry

authors： Bogachev AV,Bertsova YV,Barquera B,Verkhovsky MI

更新日期：2001-06-19 00:00:00

abstract：:The diffusion of protons at the immediate vicinity of (less than 10 A from) a phospholipid membrane is studied by the application of the laser-induced proton pulse. A light-sensitive proton emitter (8-hydroxypyrene-1,3,6-trisulfonate) was trapped exclusively in the hydration layers of multilamellar vesicles made of eg...

journal_title：Biochemistry

authors： Gutman M,Nachliel E,Moshiach S

更新日期：1989-04-04 00:00:00

abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...

journal_title：Biochemistry

authors： Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1999-01-19 00:00:00