Association of botulinum neurotoxin serotypes a and B with synaptic vesicle protein complexes.

abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...

journal_title：Biochemistry

authors： Sparrow JT,Pownall HJ,Hsu FJ,Blumenthal LD,Culwell AR,Gotto AM

更新日期：1977-12-13 00:00:00

abstract：:The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...

journal_title：Biochemistry

authors： Kauffman D,Hofmann T,Bennick A,Keller P

更新日期：1986-05-06 00:00:00

abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...

journal_title：Biochemistry

authors： Smith BC,Denu JM

更新日期：2007-12-18 00:00:00

abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...

journal_title：Biochemistry

authors： Plotnick MI,Mayne L,Schechter NM,Rubin H

更新日期：1996-06-11 00:00:00

abstract：:Protein tyrosine kinases (PTKs) are important regulators of mammalian cell function and their own activities are tightly regulated. Underlying their tight regulation, all PTKs contain multiple regulatory domains in addition to a catalytic domain. C-terminal Src kinase (Csk) contains a catalytic domain and a regulatory...

journal_title：Biochemistry

authors： Lin X,Ayrapetov MK,Lee S,Parang K,Sun G

更新日期：2005-02-08 00:00:00

abstract：:Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...

journal_title：Biochemistry

authors： Spooner PJ,Watts A

更新日期：1991-04-23 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...

journal_title：Biochemistry

authors： Ramamoorthy A,Kandasamy SK,Lee DK,Kidambi S,Larson RG

更新日期：2007-01-30 00:00:00

abstract：:The effect of both antimitotic drugs and nucleotide analogues on the magnesium-induced self-association of purified tubulin into 42S double rings has been examined by sedimentation velocity. In the absence of magnesium, all complexes sedimented as the 5.8S species. The binding of colchicine to tubulin led to a small b...

journal_title：Biochemistry

authors： Shearwin KE,Timasheff SN

更新日期：1992-09-01 00:00:00

abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...

journal_title：Biochemistry

authors： Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

更新日期：1987-08-11 00:00:00

abstract：:(R)-N(delta)-(N'-Sulfodiaminophosphinyl)-L-ornithine (PSorn) is the active component of a phytotoxin, called phaseolotoxin, produced by Pseudomonas savastanoi pv. phaseolicola. PSorn acts as a potent transition state (TS) inhibitor of ornithine transcarbamoylase (OTCase, E.C. 2.1.3.3) that binds to the OTCase from Esc...

journal_title：Biochemistry

authors： Templeton MD,Reinhardt LA,Collyer CA,Mitchell RE,Cleland WW

更新日期：2005-03-22 00:00:00

abstract：:The exchangeability of apolipoprotein (apo) E between lipoprotein particles such as very low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) is critical for lipoprotein metabolism, but despite its importance, the kinetics and mechanism of apoE-lipoprotein interaction are not known. We have used surface p...

journal_title：Biochemistry

authors： Nguyen D,Dhanasekaran P,Phillips MC,Lund-Katz S

更新日期：2009-04-07 00:00:00

abstract：:Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...

journal_title：Biochemistry

authors： Nishimura S,Sasaki J,Kandori H,Matsuda T,Fukada Y,Maeda A

更新日期：1996-10-15 00:00:00

abstract：:Solvent kinetic isotope effect studies of electron transfer within xanthine oxidase have been performed, using a stopped-flow pH-jump technique to perturb the distribution of reducing equivalents within partially reduced enzyme and follow the kinetics of reequilibration spectrophotometrically. It is found that the rat...

journal_title：Biochemistry

authors： Hille R

更新日期：1991-09-03 00:00:00

abstract：:We have used site-directed mutagenesis to alter the [Fe-S] cluster composition of Escherichia coli dimethyl sulfoxide (DMSO) reductase (DmsABC). The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [Cammack, R., & Weiner, ...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1991-08-27 00:00:00

abstract：:Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...

journal_title：Biochemistry

authors： Kisiel W,Davie EW

更新日期：1975-11-04 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:Antisera were raised in rabbits against the 40-kDa alpha subunit of bovine lung GTP-binding protein, which were identified as the alpha subunit of Gi2 (Gi2 alpha) by the analysis of the partial amino acid sequence. Antibodies were purified with a Gi2 alpha-coupled Sepharose column and then were passed through a Gi1 al...

journal_title：Biochemistry

authors： Asano T,Morishita R,Semba R,Itoh H,Kaziro Y,Kato K

更新日期：1989-05-30 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:The antimicrobial peptide MSI-78 serves as a model system for studying interactions of bioactive peptides with membranes. Using a series of MSI-78 peptides that incorporate l-4,4,4-trifluoroethylglycine, a small and sensitive (19)F nuclear magnetic resonance probe, we investigated how the local structure and dynamics ...

journal_title：Biochemistry

authors： Suzuki Y,Buer BC,Al-Hashimi HM,Marsh EN

更新日期：2011-07-12 00:00:00

abstract：:The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...

journal_title：Biochemistry

authors： Cai S,Stevens SY,Budor AP,Zuiderweg ER

更新日期：2003-09-30 00:00:00

abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

journal_title：Biochemistry

authors： McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

更新日期：2011-09-06 00:00:00

abstract：:5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...

journal_title：Biochemistry

authors： Gray JA,Compton-Toth BA,Roth BL

更新日期：2003-09-16 00:00:00

abstract：:S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

journal_title：Biochemistry

authors： Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

更新日期：2009-07-14 00:00:00

abstract：:Protein enzymes often use ionizable side chains, such as histidine, for general acid-base catalysis because the imidazole pK(a) is near neutral pH. RNA enzymes, on the other hand, are comprised of nucleotides which do not have apparent pK(a) values near neutral pH. Nevertheless, it has been recently shown that cytidin...

journal_title：Biochemistry

authors： Ravindranathan S,Butcher SE,Feigon J

更新日期：2000-12-26 00:00:00

abstract：:The structural factors responsible for the extraordinary rate enhancement ( approximately 10(17)) of the reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) have not been defined. Catalysis requires a conformational change that closes an active site loop and "clamps" the orotate base proximal to hyd...

journal_title：Biochemistry

authors： Wood BM,Amyes TL,Fedorov AA,Fedorov EV,Shabila A,Almo SC,Richard JP,Gerlt JA

更新日期：2010-05-04 00:00:00

abstract：:A specific, high-affinity binding site for ammodytoxin C in synaptic membranes from bovine cerebral cortex was detected and partially characterized. Equilibrium binding analysis revealed a single population of [125I]ammodytoxin C acceptors with the following binding parameters: Kd = 6.0 nM and Bmax = 5.7 pmol/mg membr...

journal_title：Biochemistry

authors： Krizaj I,Dolly JO,Gubensek F

更新日期：1994-11-22 00:00:00

abstract：:A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...

journal_title：Biochemistry

authors： Flynn PF,Bieber Urbauer RJ,Zhang H,Lee AL,Wand AJ

更新日期：2001-06-05 00:00:00

abstract：:The crystal structure of ferredoxin-NADP+ reductase (FNR) suggests that Ser96 is directly involved in hydride transfer between the isoalloxazine moiety of FAD and the nicotinamide ring of NADP(H). To probe its role, Ser96 has been mutated to valine (S96V) and glycine (S96G). These mutations primarily affected the inte...

journal_title：Biochemistry

authors： Aliverti A,Bruns CM,Pandini VE,Karplus PA,Vanoni MA,Curti B,Zanetti G

更新日期：1995-07-04 00:00:00