1H and 15N magnetic resonance assignments, secondary structure, and tertiary fold of Escherichia coli DnaJ(1-78).

abstract：:Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...

journal_title：Biochemistry

authors： Ciubotaru M,Koudelka GB

更新日期：2003-04-15 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...

journal_title：Biochemistry

authors： Pearce LL,Utecht RE,Kurtz DM Jr

更新日期：1987-12-29 00:00:00

abstract：:We have studied the competition between Na+ and K+ for coordination by G quartets using the oligonucleotide d(G3T4G3) as a model system. d(G3T4G3) forms a dimeric foldback structure containing three G quartets in the presence of either NaCl or KCl. Proton chemical shifts, which are particular to the species of coordin...

journal_title：Biochemistry

authors： Hud NV,Smith FW,Anet FA,Feigon J

更新日期：1996-12-03 00:00:00

abstract：:Using 125I-labeled phenol-alanine sparsomycin, an analogue of sparsomycin having higher biological activity than the unmodified antibiotic, we studied the requirements and the characteristics of its interaction with the ribosome. The drug does not bind to either isolated ribosomal subunits or reconstituted whole ribos...

journal_title：Biochemistry

authors： Lazaro E,van den Broek LA,San Felix A,Ottenheijm HC,Ballesta JP

更新日期：1991-10-08 00:00:00

abstract：:Treatment of murine leukemia virus reverse transcriptase (MuLV RT) with potassium ferrate, an oxidizing agent known to oxidize amino acids involved in phosphate binding domains of proteins, results in the irreversible inactivation of both the DNA polymerase and the RNase H activities. Significant protection from ferra...

journal_title：Biochemistry

authors： Reddy G,Nanduri VB,Basu A,Modak MJ

更新日期：1991-08-20 00:00:00

abstract：:High-affinity mu-conotoxin block of skeletal muscle Na+ channels depends on an arginine at position 13 (Arg-13). To understand both the mechanism of toxin interaction and the general structure of its binding site in the channel mouth, we examined by thermodynamic mutant cycle analysis the interaction between the criti...

journal_title：Biochemistry

authors： Chang NS,French RJ,Lipkind GM,Fozzard HA,Dudley S Jr

更新日期：1998-03-31 00:00:00

abstract：:Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...

journal_title：Biochemistry

authors： Needham D,McIntosh TJ,Evans E

更新日期：1988-06-28 00:00:00

abstract：:By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...

journal_title：Biochemistry

authors： Nagaoka M,Sugiura Y

更新日期：1996-07-02 00:00:00

abstract：:The reaction of cytochrome c oxidase with dioxygen has been studied by means of time-resolved measurements of electrical membrane potential (DeltaPsi). Microsecond time resolution was achieved by starting with the CO-inhibited enzyme, which was photolyzed after addition of oxygen. The time course of the reaction could...

journal_title：Biochemistry

authors： Jasaitis A,Verkhovsky MI,Morgan JE,Verkhovskaya ML,Wikström M

更新日期：1999-03-02 00:00:00

abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...

journal_title：Biochemistry

authors： Tiepold M,Kliche W,Pfannstiel J,Faulstich H

更新日期：2000-02-15 00:00:00

abstract：:We previously showed that estrogen-related receptor alpha1 (ERRalpha1) can compete with estrogen receptor alpha (ERalpha) for binding to estrogen response elements (EREs), repressing transcription in the mammary carcinoma cell line MCF-7. Given that ERRalpha1 can function in the absence of ligands and exists as a phos...

journal_title：Biochemistry

authors： Vu EH,Kraus RJ,Mertz JE

更新日期：2007-08-28 00:00:00

abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

journal_title：Biochemistry

authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

更新日期：2017-12-26 00:00:00

abstract：:Replication protein A (RPA) is multisubunit single-stranded DNA-binding protein required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Human RPA is a stable complex of three subunits of 70, 32, and 14 kDa (RPA70, RPA32, and RPA14, respectively). We examined the stru...

journal_title：Biochemistry

authors： Gomes XV,Henricksen LA,Wold MS

更新日期：1996-04-30 00:00:00

abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

journal_title：Biochemistry

authors： Echtay KS,Bienengraeber M,Klingenberg M

更新日期：1997-07-08 00:00:00

abstract：:Respiratory complex I couples the electron transfer exclusively from NADH to a quinone with the translocation of protons across the membrane. However, Escherichia coli adapts to imposed high cellular NADPH concentrations by selecting the mutations E183A(F) and E183G(F) that lead to a high catalytic efficiency of compl...

journal_title：Biochemistry

authors： Frick K,Schulte M,Friedrich T

更新日期：2015-05-12 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Glycogen synthase kinase 3β (GSK-3β) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3β is regulated via multiple pathways and sites. We performed all-atom ...

journal_title：Biochemistry

authors： Buch I,Fishelovitch D,London N,Raveh B,Wolfson HJ,Nussinov R

更新日期：2010-12-28 00:00:00

abstract：:To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...

journal_title：Biochemistry

authors： Qian W,Sun YL,Wang YH,Zhuang JH,Xie Y,Huang ZX

更新日期：1998-10-06 00:00:00

abstract：:Recombinant human ferrochelatase has been expressed in Escherichia coli and purified to homogeneity. Metal analyses revealed approximately 2 mol of non-heme Fe per mol of the purified enzyme (M(r) = 40,000). The UV-visible absorption spectrum of the purified enzyme consists of a protein absorption at 278 nm (epsilon a...

journal_title：Biochemistry

authors： Dailey HA,Finnegan MG,Johnson MK

更新日期：1994-01-18 00:00:00

abstract：:A new class of eukaryotic protein kinases that are not homologous to members of the serine/threonine/tyrosine protein kinase superfamily was recently identified [Futey, L. M., et al. (1995) J. Biol. Chem. 270, 523-529; Ryazanov, A. G., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 4884-4889]. This class includes euk...

journal_title：Biochemistry

authors： Pavur KS,Petrov AN,Ryazanov AG

更新日期：2000-10-10 00:00:00

abstract：:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in ...

journal_title：Biochemistry

authors： Wan C,Kiessling V,Cafiso DS,Tamm LK

更新日期：2011-04-05 00:00:00

abstract：:We have studied the kinetics of transfer of glucocerebroside between phospholipid bilayers by using pyrene and 3H-labeled glucocerebroside incorporated into dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) bilayers. Pyrene-labeled glucocerebroside (PyrCer) molecules are able to form an e...

journal_title：Biochemistry

authors： Correa-Freire MC,Barenholz Y,Thompson TE

更新日期：1982-03-16 00:00:00

abstract：:Human melanin-concentrating hormone (hMCH) and many of its analogues are potent but nonspecific ligands for human melanin-concentrating hormone receptors 1 and 2 (hMCH-1R and hMCH-2R). To differentiate between the physiological functions of these receptors, selective antagonists are needed. In this study, analogues of...

journal_title：Biochemistry

authors： Bednarek MA,Hreniuk DL,Tan C,Palyha OC,MacNeil DJ,Van der Ploeg LH,Howard AD,Feighner SD

更新日期：2002-05-21 00:00:00

abstract：:The bovine prothrombin gene was characterized by Southern blot analysis of bovine genomic DNA using bovine prothrombin cDNA fragments as hybridization probes. These analyses suggested that the bovine genome contains a single prothrombin gene that is at least 10 kilobase pairs (kbp) in size. To characterize the gene mo...

journal_title：Biochemistry

authors： Irwin DM,Ahern KG,Pearson GD,MacGillivray RT

更新日期：1985-11-19 00:00:00

abstract：:Duplication of a single β-strand that forms part of a β-sheet in photoactive yellow protein (PYP) was found to produce two approximately isoenergetic protein conformations, in which either the first or the second copy of the duplicated β-strand participates in the β-sheet. Whereas one conformation (big-loop) is more s...

journal_title：Biochemistry

authors： Kumar A,Nokhrin S,Woloschuk RM,Woolley GA

更新日期：2018-07-17 00:00:00

abstract：:Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...

journal_title：Biochemistry

authors： Kang YN,Tran A,White RH,Ealick SE

更新日期：2007-05-01 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00