Partitioning of synaptotagmin I C2 domains between liquid-ordered and liquid-disordered inner leaflet lipid phases.


:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in presynaptic membrane fusion. Because SNAREs can be organized into small cholesterol-dependent clusters in membranes, it is important to determine whether the C2 domains of synaptotagmin target membrane domains with different cholesterol contents. To address this question, we used a previously developed asymmetric two-phase lipid bilayer system to investigate the membrane binding and lipid phase targeting of soluble C2A and C2AB domains of synaptotagmin. We found that both domains target more disordered cholesterol-poor domains better than highly ordered cholesterol-rich domains. The selectivity is greatest (∼3-fold) for C2A binding to disordered domains that are formed in the presence of 5 mol % PIP(2) and 15 mol % PS. It is smallest (∼1.4-fold) for C2AB binding to disordered domains that are formed in the presence of 40 mol % PS. In the course of these experiments, we also found that C2A domains in the presence of Ca(2+) and C2AB domains in the absence of Ca(2+) are quite reliable reporters of the acidic lipid distribution between ordered and disordered lipid phases. Accordingly, PS prefers the liquid-disordered phase over the liquid-ordered phase by ∼2-fold, but PIP(2) has an up to 3-fold preference for the liquid-disordered phase.






Wan C,Kiessling V,Cafiso DS,Tamm LK




Has Abstract


2011-04-05 00:00:00












  • Surface loops adjacent to the cation-binding site of the complement factor B von Willebrand factor type A module determine C3b binding specificity.

    abstract::The interaction of factor B with C3b deposited on the surface of pathogens is the first step in the activation of the alternative complement pathway. The role of the von Willebrand factor type A (VWFA) module of factor B in this interaction has been investigated by generating three chimeras, Ch1-Ch3, in which surface ...


    pub_type: 杂志文章


    authors: Tuckwell DS,Xu Y,Newham P,Humphries MJ,Volanakis JE

    更新日期:1997-06-03 00:00:00

  • Pre-steady-state kinetics of the microtubule-kinesin ATPase.

    abstract::The pre-steady-state kinetics of the microtubule-kinesin ATPase were investigated by chemical-quench flow methods using the Drosophila kinesin motor domain (K401) expressed in Escherichia coli [Gilbert, S. P., & Johnson, K. A. (1993) Biochemistry 32, 4677-4684]. The results define a minimal mechanism: M.K + ATP in equ...


    pub_type: 杂志文章


    authors: Gilbert SP,Johnson KA

    更新日期:1994-02-22 00:00:00

  • Phosphoprotein particles: calcium and inorganic phosphate binding structures.

    abstract::Phosphoprotein particles were isolated in their native state from the physiological fluid of the estuarine clam Rangia cuneata , and the characteristics of the mineral ion-protein complex which constitutes the native particle were investigated by using mineral ion binding and mineral ion exchange techniques. The parti...


    pub_type: 杂志文章


    authors: Marsh ME,Sass RL

    更新日期:1984-03-27 00:00:00

  • Amyloid formation via supramolecular peptide assemblies.

    abstract::Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...


    pub_type: 杂志文章


    authors: Moore RA,Hayes SF,Fischer ER,Priola SA

    更新日期:2007-06-19 00:00:00

  • Interaction of cytochrome c with flavocytochrome b2.

    abstract::Flavocytochrome b2 from Saccharomyces cerevisiae couples L-lactate dehydrogenation to cytochrome c reduction. At 25 degrees C, 0.10 M ionic strength, and saturating L-lactate concentration, the turnover rate is 207 s-1 [per cytochrome c reduced; Miles, C. S., Rouviere, N., Lederer, F., Mathews, F. S., Reid, G. A., Bla...


    pub_type: 杂志文章


    authors: Daff S,Sharp RE,Short DM,Bell C,White P,Manson FD,Reid GA,Chapman SK

    更新日期:1996-05-21 00:00:00

  • Chemical modification of tryptophan residues in Escherichia coli succinyl-CoA synthetase. Effect on structure and enzyme activity.

    abstract::Succinyl-CoA synthetase of Escherichia coli is an alpha 2 beta 2 protein containing active sites at the interfaces between alpha- and beta-subunits. The alpha-subunit contains a histidine residue that is phosphorylated during the reaction. The beta-subunit binds coenzyme A and probably succinate [see Nishimura, J. S. ...


    pub_type: 杂志文章


    authors: Ybarra J,Prasad AR,Nishimura JS

    更新日期:1986-11-04 00:00:00

  • Functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily.

    abstract::The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...


    pub_type: 杂志文章


    authors: Ornelas A,Korczynska M,Ragumani S,Kumaran D,Narindoshvili T,Shoichet BK,Swaminathan S,Raushel FM

    更新日期:2013-01-08 00:00:00

  • Comparison of the structural changes occurring during the primary phototransition of two different channelrhodopsins from Chlamydomonas algae.

    abstract::Channelrhodopsins (ChRs) from green flagellate algae function as light-gated ion channels when expressed heterologously in mammalian cells. Considerable interest has focused on understanding the molecular mechanisms of ChRs to bioengineer their properties for specific optogenetic applications such as elucidating the f...


    pub_type: 杂志文章


    authors: Ogren JI,Yi A,Mamaev S,Li H,Lugtenburg J,DeGrip WJ,Spudich JL,Rothschild KJ

    更新日期:2015-01-20 00:00:00

  • Binding of bleomycin to DNA: intercalation of the bithiazole rings.

    abstract::At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...


    pub_type: 杂志文章


    authors: Povirk LF,Hogan M,Dattagupta N

    更新日期:1979-01-09 00:00:00

  • One-Enzyme Reverse Transcription qPCR Using Taq DNA Polymerase.

    abstract::Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine leukemia virus reverse transcriptase and has in the past been sh...


    pub_type: 杂志文章


    authors: Bhadra S,Maranhao AC,Paik I,Ellington AD

    更新日期:2020-12-15 00:00:00

  • Structural insights into the two sequential folding transition states of the PB1 domain of NBR1 from Φ value analysis and biased molecular dynamics simulations.

    abstract::The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...


    pub_type: 杂志文章


    authors: Chen P,Evans CL,Hirst JD,Searle MS

    更新日期:2011-01-11 00:00:00

  • Helix 8 and helix 10 are involved in substrate recognition in the rat monocarboxylate transporter MCT1.

    abstract::Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...


    pub_type: 杂志文章


    authors: Rahman B,Schneider HP,Bröer A,Deitmer JW,Bröer S

    更新日期:1999-08-31 00:00:00

  • Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system.

    abstract::In Saccharomyces cerevisiae, the histidine-containing phosphotransfer (HPt) protein YPD1 transfers phosphoryl groups between the three different response regulator domains of SLN1, SSK1, and SKN7 (designated R1, R2, and R3, respectively). Together these proteins form a branched histidine-aspartic acid phosphorelay sys...


    pub_type: 杂志文章


    authors: Janiak-Spens F,Cook PF,West AH

    更新日期:2005-01-11 00:00:00

  • Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose.

    abstract::Beta-1,4-galactosyltransferase (beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide. Crystallographic studies on bovine beta4Gal-T1 have shown that the primary metal binding site is located in...


    pub_type: 杂志文章


    authors: Ramakrishnan B,Boeggeman E,Qasba PK

    更新日期:2004-10-05 00:00:00

  • Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels.

    abstract::Oxygen binding curves of sol-gel-encapsulated deoxy human adult hemoglobin (HbA) have previously revealed two distinct noncooperative populations with oxygen binding affinities approximately 1000 and 100 times lower than that of the high-affinity R state. The two populations which have been termed the low-affinity (LA...


    pub_type: 杂志文章


    authors: Samuni U,Dantsker D,Juszczak LJ,Bettati S,Ronda L,Mozzarelli A,Friedman JM

    更新日期:2004-11-02 00:00:00

  • The betagamma-crystallin superfamily contains a universal motif for binding calcium.

    abstract::The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...


    pub_type: 杂志文章


    authors: Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

    更新日期:2009-12-29 00:00:00

  • Allocrite Sensing and Binding by the Breast Cancer Resistance Protein (ABCG2) and P-Glycoprotein (ABCB1).

    abstract::The ATP binding cassette (ABC) transporters ABCG2 and ABCB1 perform ATP hydrolysis-dependent efflux of structurally highly diverse compounds, collectively called allocrites. Whereas much is known about allocrite-ABCB1 interactions, the chemical nature and strength of ABCG2-allocrite interactions have not yet been asse...


    pub_type: 杂志文章


    authors: Xu Y,Egido E,Li-Blatter X,Müller R,Merino G,Bernèche S,Seelig A

    更新日期:2015-10-13 00:00:00

  • Structure of the potassium form of CGCGAATTCGCG: DNA deformation by electrostatic collapse around inorganic cations.

    abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...


    pub_type: 杂志文章


    authors: Shui X,Sines CC,McFail-Isom L,VanDerveer D,Williams LD

    更新日期:1998-12-01 00:00:00

  • High-resolution mapping of carcinogen binding sites on DNA.

    abstract::We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...


    pub_type: 杂志文章


    authors: Boles TC,Hogan ME

    更新日期:1986-05-20 00:00:00

  • Delineation of two functionally distinct gammaPDE binding sites on the bovine retinal cGMP phosphodiesterase by a mutant gammaPDE subunit.

    abstract::The gamma subunit of the retinal cGMP phosphodiesterase (gammaPDE) acts as an inhibitor of phosphodiesterase (PDE) catalytic activity and mediates enzyme regulation by the alpha subunit of the GTP-binding protein transducin (alphaT). In this work, we describe a full length, doubly point-mutated gamma subunit, C68S, Y8...


    pub_type: 杂志文章


    authors: Berger AL,Cerione RA,Erickson JW

    更新日期:1999-01-26 00:00:00

  • Thermodynamics of a transition state analogue inhibitor binding to Escherichia coli chorismate mutase: probing the charge state of an active site residue and its role in inhibitor binding and catalysis.

    abstract::Electrostatic interactions play important roles in the catalysis of chorismate to prephenate by chorismate mutase. Mutation of Gln88 to glutamate in the monofunctional chorismate mutase from Escherichia coli results in an enzyme with a pH profile of activity significantly different from that of the wild type protein. ...


    pub_type: 杂志文章


    authors: Lee AY,Zhang S,Kongsaeree P,Clardy J,Ganem B,Erickson JW,Xie D

    更新日期:1998-06-23 00:00:00

  • Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. Copurification of HPr and alpha 1-6 glucan.

    abstract::A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...


    pub_type: 杂志文章


    authors: Dooijewaard G,Roossien FF,Robillard GT

    更新日期:1979-07-10 00:00:00

  • Circular dichroism spectroscopy of the intermediates that precede the rate-limiting step of the refolding pathway of bovine pancreatic trypsin inhibitor. Relationship of conformation and the refolding pathway.

    abstract::Circular dichroism spectra of the partially folded trapped intermediates were measured in order to aid in the elucidation of the conformational forces which determine a nonrandom, nonsequential pathway of disulfide bond formation upon refolding of bovine pancreatic trypsin inhibitor. Whatever conformation was responsi...


    pub_type: 杂志文章


    authors: Kosen PA,Creighton TE,Blout ER

    更新日期:1983-05-10 00:00:00

  • Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST.

    abstract::Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...


    pub_type: 杂志文章


    authors: Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

    更新日期:2011-02-01 00:00:00

  • Phosphoinositide-specific phospholipase C-delta 1: effect of monolayer surface pressure and electrostatic surface potentials on activity.

    abstract::We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...


    pub_type: 杂志文章


    authors: Rebecchi M,Boguslavsky V,Boguslavsky L,McLaughlin S

    更新日期:1992-12-29 00:00:00

  • Rapid plasmenylethanolamine-selective fusion of membrane bilayers catalyzed by an isoform of glyceraldehyde-3-phosphate dehydrogenase: discrimination between glycolytic and fusogenic roles of individual isoforms.

    abstract::Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted hexagona...


    pub_type: 杂志文章


    authors: Glaser PE,Gross RW

    更新日期:1995-09-26 00:00:00

  • Characterization of Lck-binding elements in the herpesviral regulatory Tip protein.

    abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...


    pub_type: 杂志文章


    authors: Bauer F,Hofinger E,Hoffmann S,Rösch P,Schweimer K,Sticht H

    更新日期:2004-11-30 00:00:00

  • Location of an alpha-helix in fragment 96-133 from bovine somatotropin by 1H NMR spectroscopy.

    abstract::By use of two-dimensional NMR techniques most of the proton resonances (greater than 90%) are assigned for the tryptic digest fragment 96-133 of bovine somatotropin in 30% 2,2,2-trifluoroethanol-d3/70% H2O. Qualitative analysis of the nuclear Overhauser enhancement (NOE) data indicates that a region of alpha-helix spa...


    pub_type: 杂志文章


    authors: Gooley PR,MacKenzie NE

    更新日期:1988-05-31 00:00:00

  • Identification of Cys139 and Glu207 as catalytically important groups in the active site of isopentenyl diphosphate:dimethylallyl diphosphate isomerase.

    abstract::Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (EC catalyzes the antarafacial [1.3] allylic rearrangement of isopentenyl diphosphate (IPP) to its electrophilic allylic isomer dimethylallyl diphosphate (DMAPP). Active-site thiols at C138 and C139 were recently identified by covalent modification u...


    pub_type: 杂志文章


    authors: Street IP,Coffman HR,Baker JA,Poulter CD

    更新日期:1994-04-12 00:00:00

  • Backbone dynamics of the glucocorticoid receptor DNA-binding domain.

    abstract::The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...


    pub_type: 杂志文章


    authors: Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

    更新日期:1992-12-08 00:00:00