Partitioning of synaptotagmin I C2 domains between liquid-ordered and liquid-disordered inner leaflet lipid phases.


:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in presynaptic membrane fusion. Because SNAREs can be organized into small cholesterol-dependent clusters in membranes, it is important to determine whether the C2 domains of synaptotagmin target membrane domains with different cholesterol contents. To address this question, we used a previously developed asymmetric two-phase lipid bilayer system to investigate the membrane binding and lipid phase targeting of soluble C2A and C2AB domains of synaptotagmin. We found that both domains target more disordered cholesterol-poor domains better than highly ordered cholesterol-rich domains. The selectivity is greatest (∼3-fold) for C2A binding to disordered domains that are formed in the presence of 5 mol % PIP(2) and 15 mol % PS. It is smallest (∼1.4-fold) for C2AB binding to disordered domains that are formed in the presence of 40 mol % PS. In the course of these experiments, we also found that C2A domains in the presence of Ca(2+) and C2AB domains in the absence of Ca(2+) are quite reliable reporters of the acidic lipid distribution between ordered and disordered lipid phases. Accordingly, PS prefers the liquid-disordered phase over the liquid-ordered phase by ∼2-fold, but PIP(2) has an up to 3-fold preference for the liquid-disordered phase.






Wan C,Kiessling V,Cafiso DS,Tamm LK




Has Abstract


2011-04-05 00:00:00












  • Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA.

    abstract::The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...


    pub_type: 杂志文章


    authors: Kruger RG,Otvos B,Frankel BA,Bentley M,Dostal P,McCafferty DG

    更新日期:2004-02-17 00:00:00

  • Characterization of the nuclease activity of Drosophila Rrp1 on phosphoglycolate- and phosphate-modified DNA 3'-termini.

    abstract::Drosophila Rrp1 includes a carboxy-terminal region homologous to Escherichia coli exonuclease III which is sufficient to repair both oxidative and alkylation damage to DNA. An apurinic/apyrimidinic endonuclease activity intrinsic to Rrp1 was characterized previously. In this work, the 3'-phosphodiesterase and 3'-phosp...


    pub_type: 杂志文章


    authors: Sander M,Huang SM

    更新日期:1995-01-31 00:00:00

  • Using saturation-recovery EPR to measure distances in proteins: applications to photosystem II.

    abstract::The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...


    pub_type: 杂志文章


    authors: Hirsh DJ,Beck WF,Innes JB,Brudvig GW

    更新日期:1992-01-21 00:00:00

  • Evidence for distinct DNA binding forms of the erythroid-specific transcription factor NF-E2.

    abstract::The transcriptional activity of the beta-globin genes is regulated by a complex genetic element, the locus control region (LCR), at the 5'-end of the beta-globin locus. Tandem binding sites for the erythroid-specific transcription factor NF-E2 are important for the transcriptional activation function of the LCR. We di...


    pub_type: 杂志文章


    authors: Lam LT,Bresnick EH

    更新日期:1995-12-19 00:00:00

  • FIONA on Caenorhabditis elegans.

    abstract::Despite much work, subcellular neurons of Caenorhabditis elegans have not been studied at nanometer resolution with millisecond time resolution. Nor has there been an effective way to immobilize C. elegans. Here we show that, without using anesthetic or paralyzing agents, fluorescence imaging with one-nanometer accura...


    pub_type: 杂志文章


    authors: Kural C,Nonet ML,Selvin PR

    更新日期:2009-06-09 00:00:00

  • Thermodynamics of nucleotide and inhibitor binding to wild-type and ispinesib-resistant forms of human kinesin spindle protein.

    abstract::Current antimitotic cancer chemotherapy based on vinca alkaloids and taxanes target tubulin, a protein required not only for mitotic spindle formation but also for the overall structural integrity of terminally differentiated cells. Among many innovations targeting specific mitotic events, inhibition of motor enzymes ...


    pub_type: 杂志文章


    authors: Sheth PR,Basso A,Duca JS,Lesburg CA,Ogas P,Gray K,Nale L,Mannarino AF,Prongay AJ,Le HV

    更新日期:2009-11-24 00:00:00

  • A reexamination of the nucleotide incorporation fidelity of DNA polymerases.

    abstract::Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...


    pub_type: 杂志文章,评审


    authors: Showalter AK,Tsai MD

    更新日期:2002-08-27 00:00:00

  • Regulation of the eel electroplax Na channel and phosphorylation of residues on amino- and carboxyl-terminal domains by cAMP-dependent protein kinase.

    abstract::Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...


    pub_type: 杂志文章


    authors: Emerick MC,Shenkel S,Agnew WS

    更新日期:1993-09-14 00:00:00

  • Differential susceptibility of transferrin glycoforms to chymotrypsin: a proteomics approach to the detection of carbohydrate-deficient transferrin.

    abstract::Transferrin exhibits heterogeneity in glycosylation characteristic of pathological changes in alcohol abuse and congenital disorders in glycosylation. This study investigated an alternative approach in the detection of carbohydrate-deficient transferrin based on the premise that glycosylation may afford some degree of...


    pub_type: 杂志文章


    authors: Valmu L,Kalkkinen N,Husa A,Rye PD

    更新日期:2005-12-13 00:00:00

  • Efficiency of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli.

    abstract::We have measured the rates and efficiencies of DNA unwinding (the number of ATP molecules hydrolyzed per DNA base pair unwound) catalyzed by the RecBC,RecBCD-K177Q (a site-directed mutant in the putative ATP-binding site in the RecD subunit), and RecBCD enzymes from Escherichia coli. The DNA unwinding rate was measure...


    pub_type: 杂志文章


    authors: Korangy F,Julin DA

    更新日期:1994-08-16 00:00:00

  • Preferential binding of H1e histone to GC-rich DNA.

    abstract::We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentiall...


    pub_type: 杂志文章


    authors: Wellman SE,Sittman DB,Chaires JB

    更新日期:1994-01-11 00:00:00

  • Michler's hydrol blue: a sensitive probe for amyloid fibril detection.

    abstract::Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...


    pub_type: 杂志文章


    authors: Kitts CC,Beke-Somfai T,Nordén B

    更新日期:2011-05-03 00:00:00

  • Structures of apolipoprotein A-II and a lipid-surrogate complex provide insights into apolipoprotein-lipid interactions.

    abstract::Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid me...


    pub_type: 杂志文章,收录出版


    authors: Kumar MS,Carson M,Hussain MM,Murthy HM

    更新日期:2002-10-01 00:00:00

  • The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily.

    abstract::Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...


    pub_type: 杂志文章


    authors: Nguyen TT,Fedorov AA,Williams L,Fedorov EV,Li Y,Xu C,Almo SC,Raushel FM

    更新日期:2009-09-22 00:00:00

  • Epidermal growth factor binding to human alpha 2-macroglobulin. Implications for alpha 2-macroglobulin-growth factor interactions.

    abstract::We have examined the binding of 125I-labeled human and mouse epidermal growth factors (EGF) to human alpha 2-macroglobulin (alpha 2M). In the presence of human neutrophil elastase, both mouse and human EGF bound to alpha 2M, whereas little binding was found to native alpha 2M. Binding was found to be predominantly cov...


    pub_type: 杂志文章


    authors: Gettins PG,Crews BC

    更新日期:1993-08-10 00:00:00

  • The complete amino acid sequence of the major component myoglobin from the arctic minke whale, Balaenoptera acutorostrata.

    abstract::The complete primary structure of the major component myoglobin from the Arctic minke whale, Balaenoptera acutorostrata, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three ...


    pub_type: 杂志文章


    authors: Lehman LD,Dwulet FE,Bogardt RA Jr,Jones BN,Gurd FR

    更新日期:1977-02-22 00:00:00

  • N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF.

    abstract::Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...


    pub_type: 杂志文章


    authors: Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

    更新日期:2012-08-28 00:00:00

  • Nitroxides block DNA scission and protect cells from oxidative damage.

    abstract::The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...


    pub_type: 杂志文章


    authors: Samuni A,Godinger D,Aronovitch J,Russo A,Mitchell JB

    更新日期:1991-01-15 00:00:00

  • Thermodynamic parameters and shape of the mycobacterial polymethylpolysaccharide-fatty acid complex.

    abstract::Properties of the mycobacterial polymethylpolysaccharide-lipid complex have been investigated by fluorometric techniques. From the dissociation constant for the O-methyglucose polysaccharide-parinaric acid complex at 293 K, a Gibbs free energy (delta G degree) of -33.65 kJ/mol was obtained. The Kd decreased with incre...


    pub_type: 杂志文章


    authors: Kiho T,Ballou CE

    更新日期:1988-07-26 00:00:00

  • Influence of histidine tag attachment on picosecond protein dynamics.

    abstract::Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...


    pub_type: 杂志文章


    authors: Thielges MC,Chung JK,Axup JY,Fayer MD

    更新日期:2011-06-28 00:00:00

  • Synthesis and spectroscopic studies of the aminoglycoside (neomycin)--perylene conjugate binding to human telomeric DNA.

    abstract::Synthesis of a novel perylene-neomycin conjugate (3) and the properties of its binding to human telomeric G-quadruplex DNA, 5'-d[AG3(T2AG3)3] (4), are reported. Various spectroscopic techniques were employed to characterize the binding of conjugate 3 to 4. A competition dialysis assay revealed that 3 preferentially bi...


    pub_type: 杂志文章


    authors: Xue L,Ranjan N,Arya DP

    更新日期:2011-04-12 00:00:00

  • Quantitative Characterization of Bivalent Probes for a Dual Bromodomain Protein, Transcription Initiation Factor TFIID Subunit 1.

    abstract::Multivalent binding is an efficient means to enhance the affinity and specificity of chemical probes targeting multidomain proteins in order to study their function and role in disease. While the theory of multivalent binding is straightforward, physical and structural characterization of bivalent binding encounters m...


    pub_type: 杂志文章


    authors: Suh JL,Watts B,Stuckey JI,Norris-Drouin JL,Cholensky SH,Dickson BM,An Y,Mathea S,Salah E,Knapp S,Khan A,Adams AT,Strahl BD,Sagum CA,Bedford MT,James LI,Kireev DB,Frye SV

    更新日期:2018-04-10 00:00:00

  • Resonance Raman spectroscopy of ribonucleotide reductase. Evidence for a deprotonated tyrosyl radical and photochemistry of the binuclear iron center.

    abstract::Native ribonucleotide reductase from Escherichia coli exhibits a resonance-enhanced Raman mode at 1498 cm-1 that is characteristic of a tyrosyl radical. The Raman frequency as well as the absorption maximum at 410 nm identifies the radical as being in a deprotonated state. The B2 subunit of ribonucleotide reductase sh...


    pub_type: 杂志文章


    authors: Backes G,Sahlin M,Sjöberg BM,Loehr TM,Sanders-Loehr J

    更新日期:1989-02-21 00:00:00

  • Structure around the cleavage site in the thrombin receptor determined by NMR spectroscopy.

    abstract::NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...


    pub_type: 杂志文章


    authors: Smith KJ,Trayer IP,Grand RJ

    更新日期:1994-05-24 00:00:00

  • Comparison of the energetics of the uncatalyzed and glutamate dehydrogenase catalyzed alpha-imino acid-alpha-amino acid interconversion.

    abstract::The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...


    pub_type: 杂志文章


    authors: Srinivasan R,Fisher HF

    更新日期:1985-09-24 00:00:00

  • Folding of the SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with three-dimensional structural modeling.

    abstract::Spike glycoprotein of SARS coronavirus (S protein) plays a pivotal role in SARS coronavirus (SARS_CoV) infection. The immunological fragment of the S protein (Ala251-His641, SARS_S1b) is believed to be essential for SARS_CoV entering the host cell through S protein-ACE-2 interaction. We have quantitatively characteriz...


    pub_type: 杂志文章


    authors: Yu C,Gui C,Luo H,Chen L,Zhang L,Yu H,Yang S,Jiang W,Shen J,Shen X,Jiang H

    更新日期:2005-02-08 00:00:00

  • Assignment of the proton NMR spectrum of reduced and oxidized thioredoxin: sequence-specific assignments, secondary structure, and global fold.

    abstract::Complete proton assignments are reported for the 1H nuclear magnetic resonance (NMR) spectrum of Escherichia coli thioredoxin in the oxidized (with active-site disulfide bridge) and reduced (with two sulfhydryl groups) states. The assignments were obtained by using an integrated assignment strategy in which spin syste...


    pub_type: 杂志文章


    authors: Dyson HJ,Holmgren A,Wright PE

    更新日期:1989-08-22 00:00:00

  • Groove- and sequence-selective alkylation of DNA by sulfonate esters tethered to lexitropsins.

    abstract::A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...


    pub_type: 杂志文章


    authors: Zhang Y,Chen FX,Mehta P,Gold B

    更新日期:1993-08-10 00:00:00

  • Carbamate formation on tubulin: CO2/bicarbonate buffers protect tubulin from inactivation by reductive methylation and carbamoylation and promote microtubule assembly at alkaline pH.

    abstract::Carbamoylation and reductive methylation of tubulin have been shown previously to inhibit microtubule assembly, probably by attack on essential internal lysine residues [Mellado, W., Slebe, J., & Maccioni, R.B. (1982) Biochem. J. 203, 675-681; Szasz, J., Burns, R., & Sternlicht, H. (1982) J. Biol. Chem. 257, 3697-3704...


    pub_type: 杂志文章


    authors: Clark RW,Volpi M,Berlin RD

    更新日期:1988-02-09 00:00:00

  • Sequential DNA Binding and Dimerization Processes of the Photosensory Protein EL222.

    abstract::EL222 is a blue light sensor protein, which consists of a light-oxygen-voltage domain as a light sensor and a LuxR-type helix-turn-helix DNA-binding domain. The reaction dynamics of the protein-DNA binding were observed for the first time using the time-resolved transient grating method. The reaction scheme was determ...


    pub_type: 杂志文章


    authors: Takakado A,Nakasone Y,Terazima M

    更新日期:2018-03-13 00:00:00