Abstract：
:Respiratory complex I couples the electron transfer exclusively from NADH to a quinone with the translocation of protons across the membrane. However, Escherichia coli adapts to imposed high cellular NADPH concentrations by selecting the mutations E183A(F) and E183G(F) that lead to a high catalytic efficiency of complex I with NADPH. Other mutations at position E183(F) resulting in an efficient NADPH oxidation were not selected. Here we show that the naturally occurring variants exhibit a remarkably low level of production of reactive oxygen species, a byproduct of NAD(P)H oxidation, that besides high catalytic efficiency might be favored by natural selection.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Frick K,Schulte M,Friedrich Tdoi
10.1021/acs.biochem.5b00160subject
Has Abstractpub_date
2015-05-12 00:00:00pages
2799-801issue
18eissn
0006-2960issn
1520-4995journal_volume
54pub_type
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