Abstract:
:We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculated using the MM-PBSA continuum solvent method, and excellent qualitative agreement with the SPR experimental data, with a correlation coefficient of 0.92, was obtained. Consistent with previous experimental findings, phosphotyrosyl peptides with the consensus sequence pYXNX showed favorable binding affinity for the Grb2. Unexpectedly, phosphotyrosyl peptides with the consensus sequence pYQQD, which had not shown any specific binding affinity for the Grb2 in earlier studies, also showed favorable binding affinity for the Grb2 in our experimental and computational analyses. Component analysis of the calculated binding free energies revealed that van der Waals interaction between the Grb2 and the phosphotyrosyl peptide was the dominant factor for specificity and binding affinity. These results indicate that current methods of estimating binding free energies are efficient for obtaining important information about protein-protein interactions, which are essential for the transmission of signals in cellular signaling pathways.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Suenaga A,Hatakeyama M,Ichikawa M,Yu X,Futatsugi N,Narumi T,Fukui K,Terada T,Taiji M,Shirouzu M,Yokoyama S,Konagaya Adoi
10.1021/bi034113hsubject
Has Abstractpub_date
2003-05-13 00:00:00pages
5195-200issue
18eissn
0006-2960issn
1520-4995journal_volume
42pub_type
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