Abstract：
:Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]amino]-acridine. Removal of the acridinyl ligand or changing the position of the nitro group from the 4- to the 2-position caused a 10-fold decrease in photocleavage efficiency, whereas a change to the 3-position caused a 30-fold reduction. The DNA cleavage was 5-fold enhanced by subsequent piperidine treatment and showed some sequence dependency with predominant cleavage at G and T residues. Furthermore, significant differences in cleavage preference were observed when the poly(methylene) linker length was changed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nielsen PE,Jeppesen C,Egholm M,Buchardt Odoi
10.1021/bi00417a021subject
Has Abstractpub_date
1988-08-23 00:00:00pages
6338-43issue
17eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002075l
更新日期：2001-02-20 00:00:00
abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a020
更新日期：1980-07-08 00:00:00
abstract：:Penicillin-binding protein 5 (PBP 5) from Escherichia coli is a well-characterized d-alanine carboxypeptidase that serves as a prototypical enzyme to elucidate the structure, function, and catalytic mechanism of PBPs. A comprehensive understanding of the catalytic mechanism underlying d-alanine carboxypeptidation and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0473004
更新日期：2005-06-14 00:00:00
abstract：:L6 is a monoclonal antitumor antibody which recognizes an epitope located in a 42-residue extracellular domain of a tumor-associated approximately 22 kDa glycoprotein antigen. The L6 mAb localizes to solid tumors in vivo and triggers complement activation and antibody-dependent cellular cytotoxicity. It has been the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00252a005
更新日期：1994-12-06 00:00:00
abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray crystallography at 2.4 Å resolution. The asym...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5006442
更新日期：2014-09-16 00:00:00
abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00390a019
更新日期：1987-08-11 00:00:00
abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00529a009
更新日期：1981-12-22 00:00:00
abstract：:Anthrax toxin receptor 1 (ANTXR1)/tumor endothelial marker 8 (TEM8) is one of two known proteinaceous cell surface anthrax toxin receptors. A metal ion dependent adhesion site (MIDAS) present in the integrin-like inserted (I) domain of ANTXR1 mediates the binding of the anthrax toxin subunit, protective antigen (PA). ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100887w
更新日期：2010-08-31 00:00:00
abstract：:The effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 RT was determined using a 66 nucleotide RNA template containing a stable 12 base pair hairpin structure. Prior to reaching the hairpin structure, the primer elongation by RT was fast and the kinetics of polymerization was not aff...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971217h
更新日期：1997-10-14 00:00:00
abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00332a026
更新日期：1985-05-21 00:00:00
abstract：:Isoforms of transforming growth factor beta (TGFbeta) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TbetaR1 and TbetaR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TbetaR...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034366a
更新日期：2003-09-02 00:00:00
abstract：:Staphylococcus aureus is a leading cause of surgical site infections that results in increased hospital stays due to the development of chronic wounds. Little is known about factors involved in S. aureus' ability to prevent wounds from healing. We discovered a novel secreted protein produced by a surgical site isolate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00523
更新日期：2015-08-11 00:00:00
abstract：:We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00582a028
更新日期：1979-07-24 00:00:00
abstract：:The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962899w
更新日期：1997-07-29 00:00:00
abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800286m
更新日期：2008-05-13 00:00:00
abstract：:The surface immobilization methods that allowed single-molecule motility experiments with native kinesin have not worked with the ncd motor protein and other kinesin-related motors. To solve this problem, a surfactant (Pluronic F108) was chemically modified with the metal-chelating group nitrilotriacetic acid (NTA) to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9829175
更新日期：1999-04-20 00:00:00
abstract：:An A81H variant of yeast iso-1-cytochrome c is prepared to test the hypothesis that the steric size of the amino acid at sequence position 81 of cytochrome c, which has evolved from Ala in yeast to Ile in mammals, slows the dynamics of the opening of the heme crevice. The A81H mutation is used both to increase steric ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501252z
更新日期：2015-03-10 00:00:00
abstract：:Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900083q
更新日期：2009-05-26 00:00:00
abstract：:The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a028
更新日期：1986-05-20 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952233m
更新日期：1996-04-30 00:00:00
abstract：:Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a024
更新日期：1992-05-26 00:00:00
abstract：:We have examined the phase partition preferences of the even chain length (n = 10-22) diacyl-3'3'-indocarbo-cyanine iodides (Cn diI) incorporated in disaturated lecithin (PC) vesicles. Two parameters were used to determine this phase preference: (i) the direction of shift of the phase transition temperature (Tm) induc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00567a039
更新日期：1980-12-23 00:00:00
abstract：:The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991974a
更新日期：2000-03-28 00:00:00
abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a018
更新日期：1978-10-31 00:00:00
abstract：:Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00527a003
更新日期：1981-11-24 00:00:00
abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00436a017
更新日期：1989-05-16 00:00:00
abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200640s
更新日期：2011-09-27 00:00:00
abstract：:Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00335a030
更新日期：1985-07-02 00:00:00
abstract：:The 2.15 A resolution crystal structure of arginase from Plasmodium falciparum, the parasite that causes cerebral malaria, is reported in complex with the boronic acid inhibitor 2(S)-amino-6-boronohexanoic acid (ABH) (K(d) = 11 microM). This is the first crystal structure of a parasitic arginase. Various protein const...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100390z
更新日期：2010-07-06 00:00:00
abstract：:The aldehyde oxidoreductase from Desulfovibrio gigas belongs to the family of molybdenum hydroxylases. Besides a molybdenum cofactor which constitutes their active site, these enzymes contain two [2Fe-2S](2+,1+) clusters which are believed to transfer the electrons provided by the substrate to an acceptor which is eit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0510025
更新日期：2005-08-30 00:00:00
