Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.

abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

journal_title：Biochemistry

authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

更新日期：1993-03-02 00:00:00

abstract：:4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated alpha-keto acids, converting unconjugated ketones to the conjugated isomers via a dienolic intermediate. The recently solved crystal structure of an isozyme of 4-OT suggests that ...

journal_title：Biochemistry

authors： Stivers JT,Abeygunawardana C,Mildvan AS,Hajipour G,Whitman CP,Chen LH

更新日期：1996-01-23 00:00:00

abstract：:Recent models for water oxidation in photosystem II postulate that the tyrosine Y(Z) radical, Y(Z)(*), abstracts both an electron and a proton from the Mn cluster during one or more steps in the catalytic cycle. This coupling of proton- and electron-transfer events is postulated to provide the necessary driving force ...

journal_title：Biochemistry

authors： Debus RJ,Campbell KA,Pham DP,Hays AM,Britt RD

更新日期：2000-05-30 00:00:00

abstract：:Disruption of the zinc-thiolate center at the active site of yeast alcohol dehydrogenase results in inactivation and zinc release. Measurements of activity, zinc release, and thiol/thiolate oxidation were used to assess the effects of biologically relevant oxidants on alcohol dehydrogenase. Alcohol dehydrogenase was i...

journal_title：Biochemistry

authors： Crow JP,Beckman JS,McCord JM

更新日期：1995-03-21 00:00:00

abstract：:Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...

journal_title：Biochemistry

authors： Mowery PC,Lozier RH,Chae Q,Tseng YW,Taylor M,Stoeckenius W

更新日期：1979-09-18 00:00:00

abstract：:A high molecular weight acyl coenzyme A (acyl-CoA) thioesterase, designated thioesterase II, has been purified 5300-fold from photoheterotrophically grown cells of Rhodopseudomonas sphaeroides. In contrast to R. sphaeroides acyl-CoA thioesterase I [Boyce, S.G., & Lueking, D.R. (1984) Biochemistry 23, 141-147], thioest...

journal_title：Biochemistry

authors： Seay T,Lueking DR

更新日期：1986-05-06 00:00:00

abstract：:The heme-regulated inhibitor (HRI) of protein synthesis becomes activated in rabbit reticulocyte lysates in response to a variety of conditions including heme-deficiency, addition of oxidants, and heat shock. Activated HRI inhibits translation by catalyzing the phosphorylation of the alpha-subunit of eukaryotic initia...

journal_title：Biochemistry

authors： Matts RL,Hurst R,Xu Z

更新日期：1993-07-27 00:00:00

abstract：:Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...

journal_title：Biochemistry

authors： Banci L,Bertini I,De la Rosa MA,Koulougliotis D,Navarro JA,Walter O

更新日期：1998-04-07 00:00:00

abstract：:Heme catabolism is an important biochemical process that many bacterial pathogens utilize to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further processing for transport out of the cell or conversion to another useful cofactor. In previous work, we presented in vitro evidence of a...

journal_title：Biochemistry

authors： LaMattina JW,Delrossi M,Uy KG,Keul ND,Nix DB,Neelam AR,Lanzilotta WN

更新日期：2017-02-14 00:00:00

abstract：:Site-directed mutants were prepared of four consecutive and highly conserved residues (His-411, Asp-412, Thr-413, Tyr-414) of an extramembrane loop that connects putative transmembrane helices IX and X of subunit I of Rhodobacter sphaeroides cytochrome c oxidase. The modified enzymes were purified and analyzed by opti...

journal_title：Biochemistry

authors： Hosler JP,Shapleigh JP,Tecklenburg MJ,Thomas JW,Kim Y,Espe M,Fetter J,Babcock GT,Alben JO,Gennis RB

更新日期：1994-02-08 00:00:00

abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

journal_title：Biochemistry

authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

更新日期：2017-09-19 00:00:00

abstract：:Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...

journal_title：Biochemistry

authors： Kyndt JA,Fitch JC,Meyer TE,Cusanovich MA

更新日期：2007-07-17 00:00:00

abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...

journal_title：Biochemistry

authors： Snitko Y,Han SK,Lee BI,Cho W

更新日期：1999-06-15 00:00:00

abstract：:The synthesis of gangliosides GM3, GM2, GM1, and GD1a solely lacking the fatty acid moiety, and thus called lysogangliosides in analogy to lysophospholipids, is described. Since a selective elimination of the fatty acid residue has not been achieved as yet, the gangliosides were first subjected to alkaline hydrolysis....

journal_title：Biochemistry

authors： Neuenhofer S,Schwarzmann G,Egge H,Sandhoff K

更新日期：1985-01-15 00:00:00

abstract：:Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To d...

journal_title：Biochemistry

authors： Finer-Moore JS,Maley GF,Maley F,Montfort WR,Stroud RM

更新日期：1994-12-27 00:00:00

abstract：:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...

journal_title：Biochemistry

authors： Webb CF,Tucker PW,Dowton SB

更新日期：1989-05-30 00:00:00

abstract：:Proline utilization A (PutA) from Escherichia coli is a multifunctional flavoprotein that is both a transcriptional repressor of the proline utilization (put) genes and a membrane-associated enzyme which catalyzes the 4-electron oxidation of proline to glutamate. Previously, proline was shown to induce PutA-membrane b...

journal_title：Biochemistry

authors： Zhang W,Zhou Y,Becker DF

更新日期：2004-10-19 00:00:00

abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...

journal_title：Biochemistry

authors： Lee Y,Esch FS,DeLuca MA

更新日期：1981-03-03 00:00:00

abstract：:In our previous work [Yang, Z. W., & Babitch, J. A. (1988) Biochemistry (preceding paper in this issue)] divalent cations were found to be more effective promoters of astroglial filament formation than were monovalent cations. To determine if one or more divalent cation binding sites were the basis for this difference...

journal_title：Biochemistry

authors： Yang ZW,Kong CF,Babitch JA

更新日期：1988-09-06 00:00:00

abstract：:The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...

journal_title：Biochemistry

authors： Fleck BA,Ling N,Chen C

更新日期：2007-09-18 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...

journal_title：Biochemistry

authors： Dwulet FE,Jones BN,Lehman LD,Gurd FR

更新日期：1977-03-08 00:00:00

abstract：:Actin depolymerizing factor (ADF)/cofilin enhances turnover of actin filaments by severing and depolymerizing filaments. A number of proteins functionally interact with ADF/cofilin to modulate the dynamics of actin filaments. Actin-interacting protein 1 (AIP1) has emerged as a conserved WD-repeat protein that specific...

journal_title：Biochemistry

authors： Ono S

更新日期：2003-11-25 00:00:00

abstract：:The Sup35 protein of Saccharomyces cerevisiae forms a prion that generates the [PSI(+)] phenotype. Its NM region governs prion status, forming self-seeding amyloid fibers in vivo and in vitro. A tryptophan mutant of Sup35 (NM(F117W)) was used to probe its aggregation. Four indicators of aggregation, Trp 117 maximum em...

journal_title：Biochemistry

authors： Palhano FL,Rocha CB,Bernardino A,Weissmuller G,Masuda CA,Montero-Lomelí M,Gomes AM,Chien P,Fernandes PM,Foguel D

更新日期：2009-07-28 00:00:00

abstract：:Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent di...

journal_title：Biochemistry

authors： Gasselhuber B,Graf MM,Jakopitsch C,Zamocky M,Nicolussi A,Furtmüller PG,Oostenbrink C,Carpena X,Obinger C

更新日期：2016-06-28 00:00:00

abstract：:The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...

journal_title：Biochemistry

authors： Fleck BA,Chen C,Yang W,Huntley R,Markison S,Nickolls SA,Foster AC,Hoare SR

更新日期：2005-11-08 00:00:00

abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

journal_title：Biochemistry

authors： Yang XY,Schulz H,Elzinga M,Yang SY

更新日期：1991-07-09 00:00:00

abstract：:The dependence of enzymatic catalysis on diffusion rates in solution was examined with regard to high specific activity carbonic anhydrase (CA II) by varying the viscosity of the reaction medium with added glycerol, sucrose, and ficoll (a copolymer of sucrose and epichlorohydrin). Responses of the Michaelis-Menten par...

journal_title：Biochemistry

authors： Pocker Y,Janjić N

更新日期：1987-05-05 00:00:00

abstract：:Human alkyladenine DNA glycosylase (AAG) locates and excises a wide variety of structurally diverse alkylated and oxidized purine lesions from DNA to initiate the base excision repair pathway. Recognition of a base lesion requires flipping of the damaged nucleotide into a relatively open active site pocket between two...

journal_title：Biochemistry

authors： Hendershot JM,Wolfe AE,O'Brien PJ

更新日期：2011-03-22 00:00:00

abstract：:Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

journal_title：Biochemistry

authors： Mukasa T,Lee Y,Knowles AF

更新日期：2005-08-23 00:00:00

abstract：:In the dark, 4-azido-2-nitrophenyl phosphate (ANPP) is a phosphate analog which behaves like a simple energy-rich phosphate donor for the sarcoplasmic reticulum (SR) Ca-ATPase. Like p-nitrophenyl phosphate (pNPP), ANPP is hydrolyzed by the enzyme only in the presence of calcium and magnesium (K0.5 and Vmax are 0.3 mM ...

journal_title：Biochemistry

authors： Lacapère JJ,Garin J

更新日期：1994-03-08 00:00:00