Expression and sequence analyses of serum amyloid A in the Syrian hamster.


:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have studied the structure and expression of SAA genes during inflammation in these animals. Two cDNA clones and one genomic clone were sequenced, suggesting that Syrian hamster SAA is encoded by at least two genes. Hepatic mRNA analyses showed that SAA was inducible in many hamster organs during acute inflammation. These studies also demonstrated that SAA mRNA for one isotype is maximally expressed at a site of local tissue damage.






Webb CF,Tucker PW,Dowton SB




Has Abstract


1989-05-30 00:00:00












  • Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica.

    abstract::The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a f...


    pub_type: 杂志文章


    authors: Nagata K,Kudo N,Abe K,Arai S,Tanokura M

    更新日期:2000-12-05 00:00:00

  • Heteronuclear NMR and crystallographic studies of wild-type and H187Q Escherichia coli uracil DNA glycosylase: electrophilic catalysis of uracil expulsion by a neutral histidine 187.

    abstract::The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...


    pub_type: 杂志文章


    authors: Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

    更新日期:1999-09-14 00:00:00

  • Photoaffinity labeling of mitochondrial adenosinetriphosphatase by 2-azidoadenosine 5'-[alpha-32P]diphosphate.

    abstract::2-Azidoadenosine 5'-diphosphate (2-azido-ADP) labeled with 32P in the alpha-position was prepared and used to photolabel the nucleotide binding sites of beef heart mitochondrial F1-ATPase. The native F1 prepared by the procedure of Knowles and Penefsky [Knowles, A. F., & Penefsky, H. S. (1972) J. Biol. Chem. 247, 6617...


    pub_type: 杂志文章


    authors: Boulay F,Dalbon P,Vignais PV

    更新日期:1985-12-03 00:00:00

  • Requirement of histidine 217 for ubiquinone reductase activity (Qi site) in the cytochrome bc1 complex.

    abstract::Folding models suggest that the highly conserved histidine 217 of the cytochrome b subunit from the cytochrome bc1 complex is close to the quinone reductase (Qi) site. This histidine (bH217) in the cytochrome b polypeptide of the photosynthetic bacterium Rhodobacter capsulatus has been replaced with three other residu...


    pub_type: 杂志文章


    authors: Gray KA,Dutton PL,Daldal F

    更新日期:1994-01-25 00:00:00

  • Low-temperature interactions of NO with the S1 and S2 states of the water-oxidizing complex of photosystem II. A novel Mn-multiline EPR signal derived from the S1 state.

    abstract::The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...


    pub_type: 杂志文章


    authors: Goussias C,Ioannidis N,Petrouleas V

    更新日期:1997-07-29 00:00:00

  • Photolysis of caged phosphatidic acid induces flagellar excision in Chlamydomonas.

    abstract::Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, which rele...


    pub_type: 杂志文章


    authors: Goedhart J,Gadella TW Jr

    更新日期:2004-04-13 00:00:00

  • Biochemical characterization of the multidrug regulator QacR distinguishes residues that are crucial to multidrug binding and induction of qacA transcription.

    abstract::Staphylococcus aureus transcription factor QacR regulates expression of the qacA multidrug efflux determinant. In response to binding cationic lipophilic compounds, including ethidium and rhodamine 6G, QacR dissociates from the qacA operator alleviating repression. Such ligand binding uniformly induces a coil-to-helix...


    pub_type: 杂志文章


    authors: Peters KM,Sharbeen G,Theis T,Skurray RA,Brown MH

    更新日期:2009-10-20 00:00:00

  • A-tract polarity dominate the curvature in flanking sequences.

    abstract::It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...


    pub_type: 杂志文章


    authors: Merling A,Sagaydakova N,Haran TE

    更新日期:2003-05-06 00:00:00

  • Transthyretin mutation Leu-55-Pro significantly alters tetramer stability and increases amyloidogenicity.

    abstract::A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...


    pub_type: 杂志文章


    authors: McCutchen SL,Colon W,Kelly JW

    更新日期:1993-11-16 00:00:00

  • Analysis of substrate-binding elements in OxlT, the oxalate:formate antiporter of Oxalobacter formigenes.

    abstract::An OxlT homology model suggests R272 and K355 in transmembrane helices 8 and 11, respectively, are critical to OxlT-mediated transport. We offer positive evidence supporting this idea by studying OxlT function after cysteine residues were separately introduced at these positions. Without further treatment, both mutant...


    pub_type: 杂志文章


    authors: Wang X,Sarker RI,Maloney PC

    更新日期:2006-08-29 00:00:00

  • Plasminogen activation is stimulated by prion protein and regulated in a copper-dependent manner.

    abstract::Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...


    pub_type: 杂志文章


    authors: Ellis V,Daniels M,Misra R,Brown DR

    更新日期:2002-06-04 00:00:00

  • Conformational changes in aerolysin during the transition from the water-soluble protoxin to the membrane channel.

    abstract::Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exchange, the structural changes associated with each step were an...


    pub_type: 杂志文章


    authors: Cabiaux V,Buckley JT,Wattiez R,Ruysschaert JM,Parker MW,van der Goot FG

    更新日期:1997-12-09 00:00:00

  • Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.

    abstract::The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-pho...


    pub_type: 杂志文章


    authors: Eads JC,Beeby M,Scapin G,Yu TW,Floss HG

    更新日期:1999-08-03 00:00:00

  • Ligand-free open-closed transitions of periplasmic binding proteins: the case of glutamine-binding protein.

    abstract::The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...


    pub_type: 杂志文章


    authors: Bermejo GA,Strub MP,Ho C,Tjandra N

    更新日期:2010-03-09 00:00:00

  • N-terminal disordered domain of Saccharomyces cerevisiae Hop1 protein is dispensable for DNA binding, bridging, and synapsis of double-stranded DNA molecules but is necessary for spore formation.

    abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...


    pub_type: 杂志文章


    authors: Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

    更新日期:2013-08-06 00:00:00

  • Solution structure of the E-domain of staphylococcal protein A.

    abstract::The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...


    pub_type: 杂志文章


    authors: Starovasnik MA,Skelton NJ,O'Connell MP,Kelley RF,Reilly D,Fairbrother WJ

    更新日期:1996-12-03 00:00:00

  • CoCl(2) induces apoptosis via the 18 kDa translocator protein in U118MG human glioblastoma cells.

    abstract::The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...


    pub_type: 杂志文章


    authors: Zeno S,Zaaroor M,Leschiner S,Veenman L,Gavish M

    更新日期:2009-06-02 00:00:00

  • Assignment of a ligand in stellacyanin by a pulsed electron paramagnetic resonance method.

    abstract::The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...


    pub_type: 杂志文章


    authors: Mims WB,Peisach J

    更新日期:1976-08-24 00:00:00

  • The basic helix-loop-helix region of human neurogenin 1 is a monomeric natively unfolded protein which forms a "fuzzy" complex upon DNA binding.

    abstract::Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...


    pub_type: 杂志文章


    authors: Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

    更新日期:2010-03-02 00:00:00

  • Step-size is determined by neck length in myosin V.

    abstract::The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...


    pub_type: 杂志文章


    authors: Sakamoto T,Yildez A,Selvin PR,Sellers JR

    更新日期:2005-12-13 00:00:00

  • Molecular characterization of the AP180 coated vesicle assembly protein.

    abstract::Recently, a new clathrin assembly protein (AP180) has been purified from coated vesicles of bovine brain (Ahle & Ungewickell, 1986). This protein has been shown to promote polymerization of clathrin into a homogeneous population of baskets under conditions where pure clathrin does not polymerize by itself. We have pur...


    pub_type: 杂志文章


    authors: Prasad K,Lippoldt RE

    更新日期:1988-08-09 00:00:00

  • Chromium(III) modification of the first metal binding site of phosphoenolpyruvate carboxykinase.

    abstract::Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) is activated by Cr2+ as the sole activator under anaerobic conditions. PEPCK was modified with Cr3+, starting with either Cr2+ or Cr3+. Cr3+ has the distinct advantage of being a paramagnetic cation that could serve as a paramagnetic probe. Activators Mn2+, Mg2+,...


    pub_type: 杂志文章


    authors: Hlavaty JJ,Nowak T

    更新日期:1998-06-02 00:00:00

  • Cholesterol efflux from macrophage foam cells is enhanced by active phospholipid transfer protein through generation of two types of acceptor particles.

    abstract::Phospholipid transfer protein (PLTP) is expressed by macrophage-derived foam cells in human atherosclerotic lesions, suggesting a regulatory role for PLTP in cellular cholesterol homeostasis. However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. PLTP is present in plasma as two for...


    pub_type: 杂志文章


    authors: Vikstedt R,Metso J,Hakala J,Olkkonen VM,Ehnholm C,Jauhiainen M

    更新日期:2007-10-23 00:00:00

  • Evidence for coupled motion and hydrogen tunneling of the reaction catalyzed by glutamate mutase.

    abstract::Glutamate mutase is one of a group of adenosylcobalamin-dependent enzymes that catalyze unusual isomerizations that proceed through organic radical intermediates generated by homolytic fission of the coenzyme's unique cobalt-carbon bond. These enzymes are part of a larger family of enzymes that catalyze radical chemis...


    pub_type: 杂志文章


    authors: Cheng MC,Marsh EN

    更新日期:2007-01-23 00:00:00

  • Identification of a new class of inhibitors of the voltage-gated potassium channel, Kv1.3, with immunosuppressant properties.

    abstract::The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...


    pub_type: 杂志文章


    authors: Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

    更新日期:2002-06-18 00:00:00

  • Fluorescence correlation spectroscopy detects galanin receptor diversity on insulinoma cells.

    abstract::Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...


    pub_type: 杂志文章


    authors: Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

    更新日期:2001-09-11 00:00:00

  • Electron paramagnetic resonance imaging of rat heart with nitroxide and polynitroxyl-albumin.

    abstract::Electron paramagnetic resonance (EPR) imaging utilizing stable nitroxyl radicals is a promising technique for measuring free radical distribution, metabolism, and tissue oxygenation in organs and tissues [Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D. J., Giannella, E., & Zweier, J. L. (1994) Proc. Natl....


    pub_type: 杂志文章


    authors: Kuppusamy P,Wang P,Zweier JL,Krishna MC,Mitchell JB,Ma L,Trimble CE,Hsia CJ

    更新日期:1996-06-04 00:00:00

  • Decreased amyloidogenicity caused by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain.

    abstract::Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...


    pub_type: 杂志文章


    authors: Kobayashi Y,Tsutsumi H,Abe T,Ikeda K,Tashiro Y,Unzai S,Kamikubo H,Kataoka M,Hiroaki H,Hamada D

    更新日期:2014-08-12 00:00:00

  • Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.

    abstract::Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...


    pub_type: 杂志文章


    authors: Davey MW,Sulkowski E,Carter WA

    更新日期:1976-02-10 00:00:00

  • Solution structure of reduced monomeric Q133M2 copper, zinc superoxide dismutase (SOD). Why is SOD a dimeric enzyme?.

    abstract::Copper, zinc superoxide dismutase is a dimeric enzyme, and it has been shown that no cooperativity between the two subunits of the dimer is operative. The substitution of two hydrophobic residues, Phe 50 and Gly 51, with two Glu's at the interface region has disrupted the quaternary structure of the protein, thus prod...


    pub_type: 杂志文章


    authors: Banci L,Benedetto M,Bertini I,Del Conte R,Piccioli M,Viezzoli MS

    更新日期:1998-08-25 00:00:00