当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > A fluorescent mutant of the NM domain of the yeast prion Sup35 provides insight into fibril formation and stability.

A fluorescent mutant of the NM domain of the yeast prion Sup35 provides insight into fibril formation and stability.

Abstract：

:The Sup35 protein of Saccharomyces cerevisiae forms a prion that generates the [PSI(+)] phenotype. Its NM region governs prion status, forming self-seeding amyloid fibers in vivo and in vitro. A tryptophan mutant of Sup35 (NM(F117W)) was used to probe its aggregation. Four indicators of aggregation, Trp 117 maximum emission, Trp polarization, thio-T binding, and light scattering increase, revealed faster aggregation at 4 degrees C than at 25 degrees C, and all indicators changed in a concerted fashion at the former temperature. Curiously, at 25 degrees C the changes were not synchronized; the first two indicators, which reflect nucleation, changed more quickly than the last two, which reflect fibril formation. These results suggest that nucleation is insensitive to temperature, whereas fibril extension is temperature dependent. As expected, aggregation is accelerated when a small fraction (5%) of the nuclei produced at 4 or 25 degrees C are added to a suspension containing the soluble NM domain, although these nuclei do not seem to propagate any structural information to the growing fibrils. Fibrils grown at 4 degrees C were less stable in GdmCl than those grown at higher temperature. However, they were both resistant to high pressure; in fact, both sets of fibrils responded to high pressure by adopting an altered conformation with a higher capacity for thio-T binding. From these data, we calculated the change in volume and free energy associated with this conformational change. AFM revealed that the fibrils grown at 4 degrees C were statistically smaller than those grown at 25 degrees C. In conclusion, the introduction of Trp 117 allowed us to more carefully dissect the effects of temperature on the aggregation of the Sup35 NM domain.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Palhano FL,Rocha CB,Bernardino A,Weissmuller G,Masuda CA,Montero-Lomelí M,Gomes AM,Chien P,Fernandes PM,Foguel D

doi

10.1021/bi9000276

subject

Has Abstract

pub_date

2009-07-28 00:00:00

pages

6811-23

issue

29

eissn

0006-2960

issn

1520-4995

journal_volume

48

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Role of the C-terminal 28 residues of beta2-microglobulin in amyloid fibril formation.

    abstract：:Beta2microglobulin (beta2m) is the major protein component of the fibrillar amyloid deposits isolated from patients diagnosed with dialysis-related amyloidosis (DRA). While investigating the molecular mechanism of amyloid fibril formation by beta2m, we found that the beta2m C-terminal peptide of 28 residues (cbeta2m) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0301486

    authors： Ivanova MI,Gingery M,Whitson LJ,Eisenberg D

    更新日期：2003-11-25 00:00:00

  • Extension of the binding motif of the Sin3 interacting domain of the Mad family proteins.

    abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355645

    authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

    更新日期：2004-01-13 00:00:00

  • Structural characterization of a (+)-trans-anti-benzo[a]pyrene-DNA adduct using NMR, restrained energy minimization, and molecular dynamics.

    abstract：:The (+)-trans-anti-benzo[a]pyrene adduct formed at the N2 amino group of guanine is the major adduct found after metabolic activation of the ubiquitous carcinogen benzo[a]pyrene. The carcinogenic and mutagenic properties of the (+)-trans-anti-BP adduct, as well as related adducts, have been extensively studied. A DNA ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00010a004

    authors： Fountain MA,Krugh TR

    更新日期：1995-03-14 00:00:00

  • Remodeling hexose-1-phosphate uridylyltransferase: mechanism-inspired mutation into a new enzyme, UDP-hexose synthase.

    abstract：:Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00499a003

    authors： Kim J,Ruzicka F,Frey PA

    更新日期：1990-11-27 00:00:00

  • Mapping global angular transitions of proteins in assemblies using multiple extrinsic reporter groups.

    abstract：:The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00116a029

    authors： Burghardt TP,Ajtai K

    更新日期：1992-01-14 00:00:00

  • Reconstruction of quaternary structures of class II tRNA synthetases by rational mutagenensis of a conserved domain.

    abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971788+

    authors： Ribas de Pouplana L,Schimmel P

    更新日期：1997-12-09 00:00:00

  • Tropomyosin stabilizes the pointed end of actin filaments by slowing depolymerization.

    abstract：:Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00447a035

    authors： Broschat KO,Weber A,Burgess DR

    更新日期：1989-10-17 00:00:00

  • SERPINB12 Is a Slow-Binding Inhibitor of Granzyme A and Hepsin.

    abstract：:The clade B/intracellular serpins protect cells from peptidase-mediated injury by forming covalent complexes with their targets. SERPINB12 is expressed in most tissues, especially at cellular interfaces with the external environment. This wide tissue distribution pattern is similar to that of granzyme A (GZMA). Becaus...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01042

    authors： Niehaus JZ,Miedel MT,Good M,Wyatt AN,Pak SC,Silverman GA,Luke CJ

    更新日期：2015-11-17 00:00:00

  • Klenow fragment-DNA interaction required for the incorporation of nucleotides opposite guanine and O6-methylguanine.

    abstract：:A mechanism by which the Klenow fragment of DNA polymerase I monitors the geometry of the base pairs may involve hydrogen bonds between the polymerase and the minor groove of the nascent base pair. The involvement of the 3-position of guanine in the template strand was examined by synthesizing oligodeoxynucleotides co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971253g

    authors： Spratt TE

    更新日期：1997-10-28 00:00:00

  • Escherichia coli single-stranded DNA binding protein is mobile on DNA: 1H NMR study of its interaction with oligo- and polynucleotides.

    abstract：:The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00320a036

    authors： Römer R,Schomburg U,Krauss G,Maass G

    更新日期：1984-12-04 00:00:00

  • Exploring the binding site of delta(lac)-acetogenin in bovine heart mitochondrial NADH-ubiquinone oxidoreductase.

    abstract：:Biochemical characterization of the inhibition mechanism of Deltalac-acetogenins synthesized in our laboratory indicated that they are a new type of inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) [Murai, M., et al. (2006) Biochemistry 45, 9778-9787]. To identify the binding site of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100454b

    authors： Kakutani N,Murai M,Sakiyama N,Miyoshi H

    更新日期：2010-06-15 00:00:00

  • Interaction of the Saccharomyces cerevisiae alpha-factor with phospholipid vesicles as revealed by proton and phosphorus NMR.

    abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00436a017

    authors： Jelicks LA,Broido MS,Becker JM,Naider FR

    更新日期：1989-05-16 00:00:00

  • Selective cytotoxicity of rhodium metalloinsertors in mismatch repair-deficient cells.

    abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2015822

    authors： Ernst RJ,Komor AC,Barton JK

    更新日期：2011-12-20 00:00:00

  • Refined 1.8-A structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein.

    abstract：:The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00091a004

    authors： Sharff AJ,Rodseth LE,Quiocho FA

    更新日期：1993-10-12 00:00:00

  • Multiple equilibria binding treatment of lipid and detergent interactions with membrane proteins. Application to cytochrome c oxidase solubilized in cholate.

    abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00376a019

    authors： Volwerk JJ,Mrsny RJ,Patapoff TW,Jost PC,Griffith OH

    更新日期：1987-01-27 00:00:00

  • Structural dynamics in the plastocyanin-photosystem 1 electron-transfer complex as revealed by mutant studies.

    abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9520141

    authors： Sigfridsson K,Young S,Hansson O

    更新日期：1996-01-30 00:00:00

  • Role of Asn112 in a Light-Driven Sodium Ion-Pumping Rhodopsin.

    abstract：:Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00741

    authors： Abe-Yoshizumi R,Inoue K,Kato HE,Nureki O,Kandori H

    更新日期：2016-10-18 00:00:00

  • Studies of inhibitor binding to Escherichia coli purine nucleoside phosphorylase using the transferred nuclear Overhauser effect and rotating-frame nuclear Overhauser enhancement.

    abstract：:NMR studies of the adenosine analog tubercidin have been carried out in the presence of Escherichia coli purine nucleoside phosphorylase (PNP) in order to characterize the conformation of the enzyme-complexed nucleoside. Although analysis of transferred NOE data at various enzyme/inhibitor ratios indicated a predomina...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00190a007

    authors： Perlman ME,Davis DG,Koszalka GW,Tuttle JV,London RE

    更新日期：1994-06-21 00:00:00

  • Intestinal fatty acid binding protein: the folding mechanism as determined by NMR studies.

    abstract：:The intestinal fatty acid binding protein is composed of two beta-sheets surrounding a large interior cavity. There is a small helical domain associated with the portal for entry of the ligand into the cavity. Denaturation of the protein has been monitored in a residue-specific manner by collecting a series of two-dim...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001518i

    authors： Hodsdon ME,Frieden C

    更新日期：2001-01-23 00:00:00

  • Pre-steady-state kinetics of the microtubule-kinesin ATPase.

    abstract：:The pre-steady-state kinetics of the microtubule-kinesin ATPase were investigated by chemical-quench flow methods using the Drosophila kinesin motor domain (K401) expressed in Escherichia coli [Gilbert, S. P., & Johnson, K. A. (1993) Biochemistry 32, 4677-4684]. The results define a minimal mechanism: M.K + ATP in equ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00173a044

    authors： Gilbert SP,Johnson KA

    更新日期：1994-02-22 00:00:00

  • Functional epitopes for site 1 of human prolactin.

    abstract：:Human prolactin (hPRL) binds two human prolactin receptor molecules, creating active heterotrimeric complexes. Receptors bind dissimilar hormone surfaces termed site 1 and site 2 in an obligate ordered process. We sought to map the functional epitopes in site 1 of hPRL. Extensive alanine mutagenesis (102 of the 199 re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101838s

    authors： Rao GV,Brooks CL

    更新日期：2011-03-01 00:00:00

  • Isatins Inhibit N5-CAIR Synthetase by a Substrate Depletion Mechanism.

    abstract：:The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00939

    authors： Streeter CC,Lin Q,Firestine SM

    更新日期：2019-04-30 00:00:00

  • DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

    abstract：:Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a037

    authors： Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

    更新日期：1986-10-21 00:00:00

  • One-disulfide intermediates of apamin exhibit native-like structure.

    abstract：:The conformations of three peptide models of the one-disulfide and fully reduced forms of apamin were characterized by using nuclear magnetic resonance (NMR) spectroscopy. Apa-2 contains the native disulfide bond between Cys3 and Cys15 in apamin with the other two cysteines replaced by alanines. Apa-1 contains the nat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00183a031

    authors： Xu X,Nelson JW

    更新日期：1994-05-03 00:00:00

  • New insights into the activation of Escherichia coli tyrosine kinase revealed by molecular dynamics simulation and biochemical analysis.

    abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900811p

    authors： Lu T,Tan H,Lee D,Chen G,Jia Z

    更新日期：2009-08-25 00:00:00

  • tRNA glycylation system from Thermus thermophilus. tRNAGly identity and functional interrelation with the glycylation systems from other phylae.

    abstract：:The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various orig...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991392t

    authors： Mazauric MH,Roy H,Kern D

    更新日期：1999-10-05 00:00:00

  • The structure of the bovine protein tyrosine phosphatase dimer reveals a potential self-regulation mechanism.

    abstract：:The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990381x

    authors： Tabernero L,Evans BN,Tishmack PA,Van Etten RL,Stauffacher CV

    更新日期：1999-09-07 00:00:00

  • Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate.

    abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00300a002

    authors： Gonzalez MA,Webb MR,Welsh KM,Cooperman BS

    更新日期：1984-02-28 00:00:00

  • Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy.

    abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100055m

    authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

    更新日期：2010-05-11 00:00:00

  • Primer length dependence of binding of DNA polymerase I Klenow fragment to template-primer complexes containing site-specific bulky lesions.

    abstract：:The binding of the benzo[a]pyrene metabolite anti-BPDE (r7, t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) to the N(2) group of 2'-deoxyguanosine residues (dG) is known to adversely affect the Michaelis-Menten primer extension kinetics catalyzed by DNA Pol I and other polymerases. In this work, the impact...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990614k

    authors： Rechkoblit O,Amin S,Geacintov NE

    更新日期：1999-09-07 00:00:00