Abstract:
:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The basis for these variations has not been understood. We report here that sequence manipulations of a structural motif (motif 1) characteristic of all class II tRNA synthetases can generate most of the evolutionary diversity of quaternary forms of class II synthetases. Thus, the principles elucidated here for quaternary structure assembly may be general.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Ribas de Pouplana L,Schimmel Pdoi
10.1021/bi971788+subject
Has Abstractpub_date
1997-12-09 00:00:00pages
15041-8issue
49eissn
0006-2960issn
1520-4995pii
bi971788+journal_volume
36pub_type
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