Comparison of lipid/gramicidin dispersions and cocrystals by Raman scattering.

abstract：:Rat liver sinusoidal endothelial cells (LECs) mediate the removal of hyaluronan (HA) from the circulation via a specific Ca(2+)-independent endocytic receptor. To characterize the receptor biochemically, detergent-soluble extracts were prepared from crude LEC membranes. Using a dot blot assay to quantitate 125I-HA bin...

journal_title：Biochemistry

authors： Yannariello-Brown J,Weigel PH

更新日期：1992-01-21 00:00:00

abstract：:Control of protein phosphatases is now understood to depend on binding to a variety of regulatory or targeting subunits to form holoenzymes with restricted localization and substrate specificity. In addition, the catalytic subunits of both type-1 and type-2 phosphatases bind specific inhibitor proteins. Here, we repor...

journal_title：Biochemistry

authors： Eto M,Karginov A,Brautigan DL

更新日期：1999-12-21 00:00:00

abstract：:There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...

journal_title：Biochemistry

authors： Lee CC,Walters RH,Murphy RM

更新日期：2007-11-06 00:00:00

abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...

journal_title：Biochemistry

authors： Brenner DG,Knowles JR

更新日期：1984-11-20 00:00:00

abstract：:Fourier transform infrared spectra of light-adapted bacteriorhodopsin exhibit a band at 1618 cm(-1) that shifts to 1625 cm(-1) upon formation of the L intermediate. It is assigned to the peptide C==O of Val49 from the fact that it shifts in [1-(13)C]valine-labeled bacteriorhodopsin and appears perturbed in the Val49--...

journal_title：Biochemistry

authors： Yamazaki Y,Tuzi S,Saitô H,Kandori H,Needleman R,Lanyi JK,Maeda A

更新日期：1996-04-02 00:00:00

abstract：:The interaction of single chain urokinase with its receptor accelerates plasminogen activator activity on cell surfaces and induces intracellular signalling in several cell types. To date, no physiologic inhibitor of this binding has been identified. We report that the binding of scuPA to its cellular receptor is inhi...

journal_title：Biochemistry

authors： al-Roof Higazi A,Aceto JF,Kniss D,Upson R,Cohen R,Dichek DA,Cines DB

更新日期：1996-05-28 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...

journal_title：Biochemistry

authors： Scarsdale JN,Kazanina G,Radaev S,Schirch V,Wright HT

更新日期：1999-06-29 00:00:00

abstract：:To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...

journal_title：Biochemistry

authors： Lee BK,Lee YH,Hauser M,Son CD,Khare S,Naider F,Becker JM

更新日期：2002-11-19 00:00:00

abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...

journal_title：Biochemistry

authors： O'Keefe JP,Dustin CM,Barber D,Snider GW,Hondal RJ

更新日期：2018-03-20 00:00:00

abstract：:The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...

journal_title：Biochemistry

authors： Chen D,Wu R,Bryan TL,Dunaway-Mariano D

更新日期：2009-01-27 00:00:00

abstract：:Mucopolysaccharides were isolated from calf cerebral microvasculature and calf aorta. The only complex carbohydrates that exhibited anticoagulant activity were heparin-like components. The biologic potencies of calf cerebral and aortic heparin-like species were 2.92 units/mg of anti-factor Xa activity and 2.85 units/m...

journal_title：Biochemistry

authors： Marcum JA,Rosenberg RD

更新日期：1984-04-10 00:00:00

abstract：:We find that the isolated, extracellular domain of tissue factor (TF1-218; sTF) exhibits only 4% of the activity of wild-type transmembrane TF (TF1-263) in an assay that measures the conversion of factor X to Xa by the TF:VIIa complex. Further, the activity of sTF is manifest only when vesicles consisting of phosphati...

journal_title：Biochemistry

authors： Waxman E,Ross JB,Laue TM,Guha A,Thiruvikraman SV,Lin TC,Konigsberg WH,Nemerson Y

更新日期：1992-04-28 00:00:00

abstract：:The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...

journal_title：Biochemistry

authors： Clore GM,Driscoll PC,Wingfield PT,Gronenborn AM

更新日期：1990-08-14 00:00:00

abstract：:Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyzes the first step in the biosynthesis of the branched-chain amino acids. In bacteria, the enzyme has a large subunit containing the catalytic machinery and a small subunit with a regulatory role. In eucaryotes, the evidence for a regulatory subunit is largely indire...

journal_title：Biochemistry

authors： Pang SS,Duggleby RG

更新日期：1999-04-20 00:00:00

abstract：:Previous studies have shown that protein 4.1 is a multifunctional protein that binds to spectrin, actin, glycophorins, the anion channel protein, and phosphatidylserine (PS). In this report, we have characterized the binding of protein 4.1 and its major proteolytic fragments to phospholipid vesicles. Pure 125I-labeled...

journal_title：Biochemistry

authors： Cohen AM,Liu SC,Lawler J,Derick L,Palek J

更新日期：1988-01-26 00:00:00

abstract：:The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F(420)-0) to form F(420)-0-glutamyl-glutama...

journal_title：Biochemistry

authors： Li H,Graupner M,Xu H,White RH

更新日期：2003-08-19 00:00:00

abstract：:A cDNA coding for a P450 expressed in human lung was isolated from a lambda gt11 library constructed from human lung mRNA using a cDNA probe to rat P450 IVA1. The cDNA-deduced amino acid sequence of this P450, designated IVB1, consisted of 511 amino acids and had a calculated molecular weight of 59,558. The IVB1 amino...

journal_title：Biochemistry

authors： Nhamburo PT,Gonzalez FJ,McBride OW,Gelboin HV,Kimura S

更新日期：1989-10-03 00:00:00

abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...

journal_title：Biochemistry

authors： Storer AC,Phelps DJ,Carey PR

更新日期：1981-06-09 00:00:00

abstract：:There is much debate whether the fatty acid substrate of lipoxygenase binds "carboxylate-end first" or "methyl-end first" in the active site of soybean lipoxygenase-1 (sLO-1). To address this issue, we investigated the sLO-1 mutants Trp500Leu, Trp500Phe, Lys260Leu, and Arg707Leu with steady-state and stopped-flow kine...

journal_title：Biochemistry

authors： Ruddat VC,Mogul R,Chorny I,Chen C,Perrin N,Whitman S,Kenyon V,Jacobson MP,Bernasconi CF,Holman TR

更新日期：2004-10-19 00:00:00

abstract：:We have measured the pH-dependent (1)H, (13)C, and (15)N NMR spectra of pyridoxal 5'-phosphate ((13)C(2)-PLP) mixed with equal amounts of either doubly (15)N-labeled diaminopropane, (15)N(α)-labeled l-lysine, or (15)N(ε)-labeled l-lysine as model systems for various intermediates of the transimination reaction in PLP-...

journal_title：Biochemistry

authors： Chan-Huot M,Sharif S,Tolstoy PM,Toney MD,Limbach HH

更新日期：2010-12-28 00:00:00

abstract：:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (EC 5.3.3.2) catalyzes the antarafacial [1.3] allylic rearrangement of isopentenyl diphosphate (IPP) to its electrophilic allylic isomer dimethylallyl diphosphate (DMAPP). Active-site thiols at C138 and C139 were recently identified by covalent modification u...

journal_title：Biochemistry

authors： Street IP,Coffman HR,Baker JA,Poulter CD

更新日期：1994-04-12 00:00:00

abstract：:Bacteriophage lambda protein phosphatase (lambdaPP) is a member of a large family of metal-containing phosphoesterases, including purple acid phosphatase, protein serine/threonine phosphatases, 5'-nucleotidase, and DNA repair enzymes such as Mre11. lambdaPP can be activated several-fold by various divalent metal ions,...

journal_title：Biochemistry

authors： Reiter TA,Reiter NJ,Rusnak F

更新日期：2002-12-24 00:00:00

abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

journal_title：Biochemistry

authors： Kahn TW,Sturtevant JM,Engelman DM

更新日期：1992-09-22 00:00:00

abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...

journal_title：Biochemistry

authors： Klosterman PS,Shah SA,Steitz TA

更新日期：1999-11-09 00:00:00

abstract：:X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichrois...

journal_title：Biochemistry

authors： Heyn MP,Dudda C,Otto H,Seiff F,Wallat I

更新日期：1989-11-14 00:00:00

abstract：:A recirculating Langendorff heart preparation is used to characterize the endogenous antithrombin associated reversibly with murine vascular endothelium. Rat hearts are perfused clear of blood and then recirculated with a physiological salt solution. Addition of heparin educes antithrombin activity continuously into t...

journal_title：Biochemistry

authors： Felsch JS,Owen WG

更新日期：1994-01-25 00:00:00

abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

journal_title：Biochemistry

authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

更新日期：2004-09-07 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:Acyl-CoA:cholesterol O-acyltransferase (EC 2.3.1.26) (ACAT) catalyzes the intracellular synthesis of cholesteryl esters from cholesterol and fatty acyl-CoA at neutral pH. Despite the probable pathophysiologic role of ACAT in vascular cholesteryl ester accumulation during atherogenesis, its mechanism of action and its ...

journal_title：Biochemistry

authors： Kinnunen PM,DeMichele A,Lange LG

更新日期：1988-09-20 00:00:00

abstract：:The kinetic and thermodynamic features of reactions catalyzed by present-day enzymes appear to be the consequence of the evolution of these proteins toward maximal catalytic effectiveness. These features are identified and analyzed (in detail for one substrate-one product enzymes) by using ideas that link the energeti...

journal_title：Biochemistry

authors： Burbaum JJ,Raines RT,Albery WJ,Knowles JR

更新日期：1989-11-28 00:00:00