Abstract:
:Mucopolysaccharides were isolated from calf cerebral microvasculature and calf aorta. The only complex carbohydrates that exhibited anticoagulant activity were heparin-like components. The biologic potencies of calf cerebral and aortic heparin-like species were 2.92 units/mg of anti-factor Xa activity and 2.85 units/mg of anti-factor IIa activity, as well as 0.56 unit/mg of anti-factor Xa activity and 0.19 unit/mg of anti-factor IIa activity, respectively. Additional experiments revealed that the anticoagulantly active aortic components were significantly present only within the intima. The above populations of heparin-like species were affinity fractionated with antithrombin. The highly active component obtained from calf cerebral microvasculature exhibited an anti-factor Xa activity of 40.7 units/mg as well as an anti-factor IIa activity of 36.8 units/mg, constituted about 4.2% of the initial mass of the starting material, and represented about 75% of the biologic potency of the starting material. The highly active component derived from calf aorta exhibited an anti-factor Xa activity of 55.4 units/mg as well as an anti-factor IIa activity of 11.3 units/mg, constituted about 0.3% of the initial mass of the starting material, and represented about 60% of the biologic potency of the starting material. The highly active cerebral microvascular species possessed a molecular weight and charge density similar to that of heparan sulfate whereas the highly active aortic species displayed a molecular weight and charge density equivalent to that of a hexadecasaccharide fragment of heparin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Marcum JA,Rosenberg RDdoi
10.1021/bi00303a023subject
Has Abstractpub_date
1984-04-10 00:00:00pages
1730-7issue
8eissn
0006-2960issn
1520-4995journal_volume
23pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00633
更新日期:2016-11-08 00:00:00
abstract::RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00256
更新日期:2018-07-03 00:00:00
abstract::A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300429p
更新日期:2012-06-12 00:00:00
abstract::Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051263e
更新日期:2005-11-29 00:00:00
abstract::The hepatic metabolism of deuteriated D-methionine has been studied in the intact, anesthetized rat using 2H NMR spectroscopy. The rate of formation of the principal labeled metabolite, [methyl-2H3]sarcosine, from the D-[methyl-2H3]methionine precursor was found to be as rapid as the rate observed previously in NMR st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00420a042
更新日期:1988-10-04 00:00:00
abstract::A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034958t
更新日期:2003-09-16 00:00:00
abstract::The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9626158
更新日期:1997-04-01 00:00:00
abstract::Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034955g
更新日期:2003-09-23 00:00:00
abstract::Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00425a013
更新日期:1988-12-13 00:00:00
abstract::To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001342k
更新日期:2000-10-24 00:00:00
abstract::Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00256a031
更新日期:1982-06-22 00:00:00
abstract::(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00404a039
更新日期:1988-02-23 00:00:00
abstract::The sterol (free and esterified cholesterol) transport activity of the small-intestinal brush border membrane was solubilized with the short-chain detergent diheptanoylphosphatidylcholine and reconstituted to an artificial membrane system (proteoliposomes). The resulting proteoliposomes were identified as unilamellar ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970625i
更新日期:1997-09-02 00:00:00
abstract::The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060990m
更新日期:2006-10-03 00:00:00
abstract::The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00559a031
更新日期:1980-09-02 00:00:00
abstract::A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a012
更新日期:1984-07-17 00:00:00
abstract::A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of pure enzyme from two rabbit livers. This abundant liver enzyme is known to bind its product tetrahydropteroylpentaglutamate (H4PteGlu5) so tightly that it does not dissociate during size exclusion chromatography. 10-Formylt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9619684
更新日期:1996-12-10 00:00:00
abstract::Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00349
更新日期:2018-07-31 00:00:00
abstract::The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1016793
更新日期:2011-01-11 00:00:00
abstract::Distinct structural features of DNA, such as the curvature of dA tracts, are important in the recognition, packaging, and regulation of DNA. Physiologically relevant concentrations of magnesium have been found to enhance the curvature of dA tract DNAs, as monitored by solution-state NMR, indicating that the structure ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010853j
更新日期:2001-08-07 00:00:00
abstract::Calmodulin and troponin C exhibit calcium-dependent binding of 1 mol/mol of dynorphin. The dissociation constants of the complexes, determined in 0.20 N KC1-1.0 mM CaCI2, pH 7.3, are 0.6 microM for calmodulin, 2.4 microM for rabbit fast skeletal muscle troponin C, and 9 microM for bovine heart troponin C. Experiments ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00306a016
更新日期:1984-05-22 00:00:00
abstract::The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00576a012
更新日期:1979-05-01 00:00:00
abstract::Circular dichroism and difference spectroscopy have been used to study dilute aqueous solutions of oxygenated, deoxygenated, and carbamoylated deoxygenated hemoglobins A and S (HbA and HbS, respectively). The spectra of HbA and HbS, in comparable state of oxygenation or carbamoylation, are identical, strongly implying...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00663a022
更新日期:1976-09-07 00:00:00
abstract::Human liver alcohol dehydrogenase [alcohol:NAD+ oxidoreductase, EC 1.1.1.1 (ADH)] catalyzes the stereospecific oxidation of different 3 beta-hydroxy-5 beta-steroids with ranges of Km from 46 to 320 microM and values of kcat from 7.0 to 72 min-1, pH 8.5. Only the class I isozymes containing gamma-subunits, gamma 1 gamm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00412a013
更新日期:1988-06-14 00:00:00
abstract::Understanding the set of rules which dictate how the primary amino acid sequence determines tertiary structure is an unsolved problem in biophysics. If it were possible to simultaneously measure all of the intramolecular distances in a protein (in real time) during a folding reaction, the "second" genetic code problem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970789z
更新日期:1997-09-16 00:00:00
abstract::We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00544a031
更新日期:1980-02-05 00:00:00
abstract::The interactions between rhodopsin molecules in a micellar detergent solution (octyl glucoside) and in reconstituted phospholipid vesicles were studied in the dark and after bleaching. Resonance energy transfer measurements were used to monitor the proximity between rhodopsin monomers conjugated with a fluorescent don...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00270a030
更新日期:1983-01-04 00:00:00
abstract::Recycling of yeast fumarase to permit repetition of its reaction chemistry requires two proton transfers and two conformational changes, in pathways that are different in detail but thematically similar in the two directions. In the malate --> fumarate direction, simple anions such as acetate accelerate the fumarate-o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9821521
更新日期:1998-12-22 00:00:00
abstract::The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901184r
更新日期:2009-09-29 00:00:00
abstract::Beta-catenin phosphorylation at serine 45 (Ser45), threonine 41 (Thr41), Ser37, and Ser33 is critical for beta-catenin degradation, and regulation of beta-catenin phosphorylation is a central part of the canonical Wnt signaling pathway. Beta-catenin mutations at Ser45, Thr41, Ser37, and Ser33 perturb beta-catenin degr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0601149
更新日期:2006-04-25 00:00:00