Abstract：
:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the reduced form of the native protein. NMR transitions were observed at 310 ppm downfield from Cd(ClO4)2 for 2Cd(II) and at 320 ppm for the 2Cd(II)--2Cu(I)-containing proteins. In each case the observed line width was 27 +/- 2 Hz. The following conclusions were drawn. (a) The very small chemical-shift difference between the two derivatives indicates that the Cd(II) binding site is very similar in both samples. It follows from this result and previous work that the imidazolato bridge is protonated on the Cu side upon reduction of the Cu ion from the II to I valence state. (b) The extremely narrow line width of the resonance in both forms suggests a virtual identity of Cd(II) bound to both subunits of the molecule. (c) The relaxation time, T1 = 1.2 s, is caused by approximately equal contributions from chemical-shift anisotropy and dipolar interactions with nearby protons.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bailey DB,Ellis PD,Fee JAdoi
10.1021/bi00544a031subject
Has Abstractpub_date
1980-02-05 00:00:00pages
591-6issue
3eissn
0006-2960issn
1520-4995journal_volume
19pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00092
更新日期：2020-03-24 00:00:00
abstract：:The catalytic (C) subunit of cAMP-dependent protein kinase interacts with two classes of inhibitors. The regulatory (R) subunits, types I and II, associate to form an inactive holoenzyme complex that is activated in response to cAMP. The C-subunit is also inhibited by small heat-stable protein kinase inhibitors (PKI's...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00213a035
更新日期：1993-12-21 00:00:00
abstract：:Fluorescence studies of the binding of IKe gene 5 protein to various polynucleotides were performed to obtain insight into the question as to what extent the binding characteristics of the gene 5 proteins of the IKe and M13 phages resemble and/or differ from each other. The fluorescence of IKe gene 5 protein is quench...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00381a037
更新日期：1987-04-07 00:00:00
abstract：:The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00299
更新日期：2020-07-21 00:00:00
abstract：:Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a020
更新日期：1983-04-12 00:00:00
abstract：:The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900038p
更新日期：2009-03-24 00:00:00
abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100365b
更新日期：2010-05-18 00:00:00
abstract：:Dodecameric glutamine synthetase (GS) from bacteria is formed from two face-to-face hexameric rings of identical subunits. These highly symmetrical aggregates from some bacteria, including Escherichia coli, "stack" in the presence of Zn2+ and other divalent ions to generate protein tubes (phase I) and subsequently ass...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00254a001
更新日期：1994-12-20 00:00:00
abstract：:CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002745n
更新日期：2001-04-24 00:00:00
abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101334j
更新日期：2010-11-09 00:00:00
abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a013
更新日期：1988-11-15 00:00:00
abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102070w
更新日期：2011-05-10 00:00:00
abstract：:Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300194f
更新日期：2012-05-01 00:00:00
abstract：:Subunit a of the Escherichia coli ATP synthase is thought to control access of protons to the ring of c subunits during proton-driven ATP synthesis. In this study, the surface exposure of subunit a in the periplasm has been examined using 3-N-maleimidyl-propionyl biocytin labeling in cells permeabilized by polymyxin B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026649t
更新日期：2003-01-21 00:00:00
abstract：:Escherichia coli DNA photolyase mediates photorepair of pyrimidine dimers occurring in UV-damaged DNA. The enzyme contains two chromophores, 1,5-dihydroflavin adenine dinucleotide (FADH2) and 5,10-methenyltetrahydrofolylpolyglutamate (MTHF). To define the roles of the two chromophores in the photochemical reaction(s) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00485a021
更新日期：1990-08-21 00:00:00
abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00320a004
更新日期：1984-12-04 00:00:00
abstract：:The two flavodoxins (YkuN and YkuP) from Bacillus subtilis have been cloned, overexpressed in Escherichia coli and purified. DNA sequencing, mass spectrometry, and flavin-binding properties showed that both YkuN and YkuP were typical short-chain flavodoxins (158 and 151 amino acids, respectively) and that an error in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049131t
更新日期：2004-10-05 00:00:00
abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800882v
更新日期：2008-11-04 00:00:00
abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00261
更新日期：2016-07-05 00:00:00
abstract：:Apolipoprotein (apo) A-I, a 243-residue, 28.1-kDa protein is a major mediator of the reverse cholesterol transport (RCT) pathway, a process that may reduce the risk of cardiovascular disease in humans. In plasma, a small fraction of lipid-free or lipid-poor apoA-I is likely a key player in the first step of RCT. There...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047717+
更新日期：2005-03-01 00:00:00
abstract：:Previous crystallographic studies in this laboratory demonstrated that immunoglobulin light chains with the same amino acid sequence can have at least two and probably three or more conformations, depending on whether the second member of an interacting pair is a light or heavy chain. If a heavy chain is not available...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00594a022
更新日期：1978-01-10 00:00:00
abstract：:Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is als...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.9b00164
更新日期：2019-12-31 00:00:00
abstract：:All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980167t
更新日期：1998-08-11 00:00:00
abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00323a016
更新日期：1985-01-15 00:00:00
abstract：:An A81H variant of yeast iso-1-cytochrome c is prepared to test the hypothesis that the steric size of the amino acid at sequence position 81 of cytochrome c, which has evolved from Ala in yeast to Ile in mammals, slows the dynamics of the opening of the heme crevice. The A81H mutation is used both to increase steric ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501252z
更新日期：2015-03-10 00:00:00
abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00828
更新日期：2020-12-22 00:00:00
abstract：:The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00141a004
更新日期：1992-07-07 00:00:00
abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3007093
更新日期：2012-10-23 00:00:00
abstract：:A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00396a049
更新日期：1987-11-03 00:00:00
abstract：:We have assayed the cross-linking of oligonucleotides containing repeated mitomycin-reactive CpG sites in order to assess the factors that enhance activation of the carbamoyl function at C10, yielding efficient mitomycin cross-linking. Drugs studied include mitomycin C (MC), N-methylmitomycin A (NMA), and the aziridin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a049
更新日期：1989-06-27 00:00:00
