Solution structure of decorin-binding protein A from Borrelia burgdorferi.


:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion of decorin are crucial to its function. We have determined the solution structure of DBPA and studied DBPA's interactions with various forms of GAGs. DBPA is determined to be a helical bundle protein consisting of five helices held together by a strong hydrophobic core. The structure also possesses a basic patch formed by portions of two helices and two flexible linkers. Low-molecular mass heparin-induced chemical shift perturbations for residues in the region as well as increases in signal intensities of select residues in their presence confirm residues in the pocket are perturbed by heparin binding. Dermatan sulfate fragments, the dominant GAG type found on decorin, were shown to have lower affinity than heparin but are still capable of binding DBPA.






Wang X




Has Abstract


2012-10-23 00:00:00












  • Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).

    abstract::Mycobacterium tuberculosis and many other members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-d-glucopyranoside (MSH or AcCys-GlcN-Ins), to act against oxidative and antibiotic stress. The biosynthesis of MSH is essential for cell growth and has bee...


    pub_type: 杂志文章


    authors: Fan F,Luxenburger A,Painter GF,Blanchard JS

    更新日期:2007-10-09 00:00:00

  • Crystal structure of Escherichia coli penicillin-binding protein 5 bound to a tripeptide boronic acid inhibitor: a role for Ser-110 in deacylation.

    abstract::Penicillin-binding protein 5 (PBP 5) from Escherichia coli is a well-characterized d-alanine carboxypeptidase that serves as a prototypical enzyme to elucidate the structure, function, and catalytic mechanism of PBPs. A comprehensive understanding of the catalytic mechanism underlying d-alanine carboxypeptidation and ...


    pub_type: 杂志文章


    authors: Nicola G,Peddi S,Stefanova M,Nicholas RA,Gutheil WG,Davies C

    更新日期:2005-06-14 00:00:00

  • Ethanol enhances agonist-induced fast desensitization in nicotinic acetylcholine receptors.

    abstract::The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...


    pub_type: 杂志文章


    authors: Wu G,Miller KW

    更新日期:1994-08-09 00:00:00

  • Urea-induced unfolding of Na,K-ATPase as evaluated by electron paramagnetic resonance spectroscopy.

    abstract::Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...


    pub_type: 杂志文章


    authors: Babavali M,Esmann M,Fedosova NU,Marsh D

    更新日期:2009-09-29 00:00:00

  • Theory for the folding and stability of globular proteins.

    abstract::Using lattice statistical mechanics, we develop theory to account for the folding of a heteropolymer molecule such as a protein to the globular and soluble state. Folding is assumed to be driven by the association of solvophobic monomers to avoid solvent and opposed by the chain configurational entropy. Theory predict...


    pub_type: 杂志文章


    authors: Dill KA

    更新日期:1985-03-12 00:00:00

  • Transbilayer exchange of phosphatidylethanolamine for phosphatidylcholine and N-acetimidoylphosphatidylethanolamine in single-walled bilayer vesicles.

    abstract::A preparation of small single-walled liposome vesicles containing a 9:1 mole ratio of phosphatidylcholine to phosphatidylethanolamine was subjected to reaction with the membrane-impermeable reagent, isethionyl acetimidate hydrochloride. This reagent converted 90% of the external phosphatidylethanolamine groups to the ...


    pub_type: 杂志文章


    authors: Roseman M,Litman BJ,Thompson TE

    更新日期:1975-11-04 00:00:00

  • Structures of the modified folates in the thermophilic archaebacteria Pyrococcus furiosus.

    abstract::The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...


    pub_type: 杂志文章


    authors: White RH

    更新日期:1993-01-26 00:00:00

  • Affinity and sequence specificity of DNA binding and site selection for primer synthesis by Escherichia coli primase.

    abstract::Primase is an essential DNA replication enzyme in Escherichia coli and responsible for primer synthesis during lagging strand DNA replication. Although the interaction of primase with single-stranded DNA plays an important role in primer RNA and Okazaki fragment synthesis, the mechanism of DNA binding and site selecti...


    pub_type: 杂志文章


    authors: Khopde S,Biswas EE,Biswas SB

    更新日期:2002-12-17 00:00:00

  • Functional tyrosyl residues of carboxypeptidase A. The effect of protein structure on the reactivity of tyrosine-198.

    abstract::Coupling of bovine carboxypeptidase A with diazotized 5-amino-1H-tetrazole increases esterase activity, decreases peptidase activity slightly, and modifies one tyrosyl residue. Subsequent nitration of the azoenzyme has no further effect on esterase activity, decreases peptidase activity markedly, and modifies a second...


    pub_type: 杂志文章


    authors: Cueni L,Riordan JF

    更新日期:1978-05-16 00:00:00

  • Synthesis and study of phosphoenolthiopyruvate.

    abstract::Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...


    pub_type: 杂志文章


    authors: Sikkema KD,O'Leary MH

    更新日期:1988-02-23 00:00:00

  • Two unique phosphorylation-driven signaling pathways crosstalk in Staphylococcus aureus to modulate the cell-wall charge: Stk1/Stp1 meets GraSR.

    abstract::The Stk1/Stp1 and GraSR signal-transduction pathways are two distinct pathways in Staphylococcus aureus that rely on a reversible phosphorylation process in transducing external stimuli intracellularly. Stk1/Stp1 is an eukaryote-like Ser/Thr kinase phosphatase pair involved in purine biosynthesis, cell-wall metabolism...


    pub_type: 杂志文章


    authors: Fridman M,Williams GD,Muzamal U,Hunter H,Siu KW,Golemi-Kotra D

    更新日期:2013-11-12 00:00:00

  • Molecular interactions of nonpeptide agonists and antagonists with the melanocortin-4 receptor.

    abstract::The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...


    pub_type: 杂志文章


    authors: Fleck BA,Chen C,Yang W,Huntley R,Markison S,Nickolls SA,Foster AC,Hoare SR

    更新日期:2005-11-08 00:00:00

  • Metamorphic response of the CLIC1 chloride intracellular ion channel protein upon membrane interaction.

    abstract::A striking feature of the CLIC (chloride intracellular channel) protein family is the ability of its members to convert between a soluble state and an integral membrane channel form. Direct evidence of the structural transition required for the CLIC protein to autonomously insert into the membrane is lacking, largely ...


    pub_type: 杂志文章


    authors: Goodchild SC,Howell MW,Littler DR,Mandyam RA,Sale KL,Mazzanti M,Breit SN,Curmi PM,Brown LJ

    更新日期:2010-06-29 00:00:00

  • Epsilon amino caproic acid inhibits streptokinase-plasminogen activator complex formation and substrate binding through kringle-dependent mechanisms.

    abstract::Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...


    pub_type: 杂志文章


    authors: Lin LF,Houng A,Reed GL

    更新日期:2000-04-25 00:00:00

  • Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer.

    abstract::Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...


    pub_type: 杂志文章


    authors: Haque ME,McIntosh TJ,Lentz BR

    更新日期:2001-04-10 00:00:00

  • Orientation and motion of spin-labels in rabbit small intestinal brush border vesicle membranes.

    abstract::The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...


    pub_type: 杂志文章


    authors: Hauser H,Gains N,Semenza G,Spiess M

    更新日期:1982-10-26 00:00:00

  • Inhibition of lipid A biosynthesis as the primary mechanism of CHIR-090 antibiotic activity in Escherichia coli.

    abstract::The deacetylation of UDP-3-O-[(R)-3-hydroxymyristoyl]-N-acetylglucosamine (UDP-3-O-acyl-GlcNAc) by LpxC is the committed reaction of lipid A biosynthesis. CHIR-090, a novel N-aroyl-l-threonine hydroxamic acid, is a potent, slow, tight-binding inhibitor of the LpxC deacetylase from the hyperthermophile Aquifex aeolicus...


    pub_type: 杂志文章


    authors: Barb AW,McClerren AL,Snehelatha K,Reynolds CM,Zhou P,Raetz CR

    更新日期:2007-03-27 00:00:00

  • Intein-Promoted Cyclization of Aspartic Acid Flanking the Intein Leads to Atypical N-Terminal Cleavage.

    abstract::Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein, which involves the removal of the intein and the ligation of the flanking polypeptides, or exteins. A DNA polymerase II intein from Pyrococcus abyssi (Pab PolII intein) can promote protein splicing in vitro on incubatio...


    pub_type: 杂志文章


    authors: Minteer CJ,Siegart NM,Colelli KM,Liu X,Linhardt RJ,Wang C,Gomez AV,Reitter JN,Mills KV

    更新日期:2017-02-28 00:00:00

  • Decay products of the S(3) state of the oxygen-evolving complex of photosystem II at cryogenic temperatures. Pathways to the formation of the S = 7/2 S(2) state configuration.

    abstract::The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...


    pub_type: 杂志文章


    authors: Ioannidis N,Petrouleas V

    更新日期:2002-07-30 00:00:00

  • Effect of phospholipid oxidation products on transbilayer movement of phospholipids in single lamellar vesicles.

    abstract::Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...


    pub_type: 杂志文章


    authors: Shaw JM,Thompson TE

    更新日期:1982-03-02 00:00:00

  • There is only one phosphoenzyme intermediate with bound calcium on the reaction pathway of the sarcoplasmic reticulum calcium ATPase.

    abstract::Identical first-order rate constants for phosphorylation of the calcium ATPase of sarcoplasmic reticulum by bound inorganic phosphate (Pi) of 25 +/- 2 s-1 with empty vesicles and 25 +/- 1 s-1 with vesicles that were passively loaded with 40 mM Ca2+ were obtained by treating the reaction as an approach to equilibrium (...


    pub_type: 杂志文章


    authors: Myung J,Jencks WP

    更新日期:1995-03-07 00:00:00

  • Structural Insight into the Discrimination between 8-Oxoguanine Glycosidic Conformers by DNA Repair Enzymes: A Molecular Dynamics Study of Human Oxoguanine Glycosylase 1 and Formamidopyrimidine-DNA Glycosylase.

    abstract::hOgg1 and FPG are the primary DNA repair enzymes responsible for removing the major guanine (G) oxidative product, namely, 7,8-dihydro-8-oxoguanine (OG), in humans and bacteria, respectively. While natural G adopts the anti conformation and forms a Watson-Crick pair with cytosine (C), OG can also adopt the syn conform...


    pub_type: 杂志文章


    authors: Sowlati-Hashjin S,Wetmore SD

    更新日期:2018-02-20 00:00:00

  • Role of the carboxyl-terminal lectin domain in self-association of galectin-3.

    abstract::Galectin-3 is a member of a large family of beta-galactoside-binding animal lectins and is composed of a carboxyl-terminal lectin domain connected to an amino-terminal nonlectin part. Previous experimental results suggest that, when bound to multivalent glycoconjugates, galectin-3 self-associates through intermolecula...


    pub_type: 杂志文章


    authors: Yang RY,Hill PN,Hsu DK,Liu FT

    更新日期:1998-03-24 00:00:00

  • Molecular cloning of bovine cardiac muscle heat-shock protein 70 kDa and its phosphorylation by cAMP-dependent protein kinase in vitro.

    abstract::The 70-kDa heat-shock protein (Hsp70) has been cloned and sequenced from bovine cardiac muscle. On the basis of sequence features, the gene corresponds to the cytoplasmic form of Hsp70. This cardiac Hsp70 cDNA clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25...


    pub_type: 杂志文章


    authors: Lakshmikuttyamma A,Selvakumar P,Anderson DH,Datla RS,Sharma RK

    更新日期:2004-10-26 00:00:00

  • Insertion of nucleotides opposite apurinic/apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides.

    abstract::M13 DNA containing 20-30 apurinic/apyrimidinic (AP) sites per intact circular molecule was prepared by growing phage on an ung- dut- Escherichia coli mutant and treating the DNA with uracil N-glycosylase. AP sites obstruct in vitro DNA synthesis catalyzed by E. coli pol I. The position at which termination of synthesi...


    pub_type: 杂志文章


    authors: Sagher D,Strauss B

    更新日期:1983-09-13 00:00:00

  • 9-cis-retinoids: biosynthesis of 9-cis-retinoic acid.

    abstract::Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...


    pub_type: 杂志文章


    authors: Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

    更新日期:2000-07-11 00:00:00

  • Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.

    abstract::The backbone dynamics of the liganded (holo) and unliganded (apo) forms of Escherichia coli-derived rat intestinal fatty acid-binding protein (I-FABP) have been characterized and compared using amide 15N relaxation and 1H exchange NMR measurements. The amide 1H/15N resonances for apo and holo I-FABP were assigned at 2...


    pub_type: 杂志文章


    authors: Hodsdon ME,Cistola DP

    更新日期:1997-02-25 00:00:00

  • The selectivity for K+ versus Na+ in DNA quadruplexes is dominated by relative free energies of hydration: a thermodynamic analysis by 1H NMR.

    abstract::We have studied the competition between Na+ and K+ for coordination by G quartets using the oligonucleotide d(G3T4G3) as a model system. d(G3T4G3) forms a dimeric foldback structure containing three G quartets in the presence of either NaCl or KCl. Proton chemical shifts, which are particular to the species of coordin...


    pub_type: 杂志文章


    authors: Hud NV,Smith FW,Anet FA,Feigon J

    更新日期:1996-12-03 00:00:00

  • Calcium-dependent regulation of caldesmon by an 11-kDa smooth muscle calcium-binding protein, caltropin.

    abstract::Caldesmon from chicken gizzard muscle has been examined for its ability to interact with caltropin using affinity chromatography and the fluorescent probe acrylodan. The action of caltropin on the inhibitory effect of caldesmon on actomyosin ATPase was also studied. Like calmodulin, caltropin could release the inhibit...


    pub_type: 杂志文章


    authors: Mani RS,McCubbin WD,Kay CM

    更新日期:1992-12-01 00:00:00

  • Kinetic mechanism of Escherichia coli asparagine synthetase B.

    abstract::Escherichia coli asparagine synthetase B (AS-B) catalyzes the synthesis of asparagine from aspartate, glutamine, and ATP. A combination of kinetic, isotopic-labeling, and stoichiometry studies have been performed to define the nature of nitrogen transfer mediated by AS-B. The results of initial rate studies were consi...


    pub_type: 杂志文章


    authors: Boehlein SK,Stewart JD,Walworth ES,Thirumoorthy R,Richards NG,Schuster SM

    更新日期:1998-09-22 00:00:00