Abstract：
:The precipitation of a monoclonal IgG2 crystalline cryoglobulin (WEB) is shown to be highly dependent on temperature and concentration. Below a critical concentration of 0.6 mg/mL there is no cryoprecipitation. The kinetics of the aggregation exhibits a concentration-dependent lag time. This evidence suggests that a nucleation event is important in the precipitation. Circular dichroism (CD) was used to investigate the conformational properties of the protein. At a low concentration (0.12 or 0.15 mg/mL), no detectable spectral changes in the far- and near-UV range were noted between 40 and 3 degrees C. However, at higher concentrations (1.21 mg/mL), a small and rapid CD change was observed in the 250-280-nm region at 3 degrees C. This indicates an intermolecular interaction that precedes the precipitation. Cryoprecipitation of WEB was also shown to be dependent on maintenance of intact interchain disulfide bonds. Only one or two interchain disulfides need be cleaved to abolish cryocrystallization and to significantly diminish the CD change at 3 degrees C. The evidence is consistent with the formation of an initial intermediate that involves interactions near the disulfide bonds in the hinge region of the cryoimmunoglobulin. In this model, cleavage of these disulfides prevents this interaction and abolishes cryoprecipitation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Scoville CD,Abraham GN,Turner DHdoi
10.1021/bi00579a027subject
Has Abstractpub_date
1979-06-12 00:00:00pages
2610-5issue
12eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Reaction of 1-oxiranylpyrene (1-OP) with DNA and the structures of the covalent and noncovalent complexes formed were studied in aqueous media (5 mM phosphate buffer with 0.1 M NaCl, pH 7) by utilizing the techniques of absorption, fluorescence and linear dichroism spectroscopy in order to gain an understanding of pos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a009
更新日期：1984-11-06 00:00:00
abstract：:Human frataxin has a vital role in the biosynthesis of iron-sulfur (Fe-S) clusters in mitochondria, and its deficiency causes the neurodegenerative disease Friedreich's ataxia. Proposed functions for frataxin in the Fe-S pathway include iron donation to the Fe-S cluster machinery and regulation of cysteine desulfurase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400443n
更新日期：2013-09-03 00:00:00
abstract：:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00435a014
更新日期：1989-05-02 00:00:00
abstract：:X-ray solution scattering data from skeletal muscle troponin C and from calmodulin have been measured. Modeling studies based on the crystal structure coordinates for these proteins show discrepancies between the solution data and the crystal structure that indicate that if the size and shape of the globular domains a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00403a011
更新日期：1988-02-09 00:00:00
abstract：:The interaction of proteoglycan monomers with hyaluronate in cartilage is mediated by a globular binding region at the N-terminus of the proteoglycan monomer; this interaction is stabilized by link protein. Sequences show that both the binding region (27% carbohydrate) and the link protein (6% carbohydrate) contain an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00108a015
更新日期：1991-11-05 00:00:00
abstract：:Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061837v
更新日期：2006-12-26 00:00:00
abstract：:FeNO vibrational frequencies were investigated for a series of myoglobin mutants using isotope-edited resonance Raman spectra of (15/14)NO adducts, which reveal the FeNO and NO stretching modes. The latter give rise to doublet bands, as a result of Fermi resonances with coincident porphyrin vibrations; these doublets ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026395b
更新日期：2003-05-06 00:00:00
abstract：:In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond ( journal_title：Biochemistry pub_type： 杂志文章 doi：10.1021/bi700904a 更新日期：2007-10-30 00:00:00
abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00180a040
更新日期：1994-04-12 00:00:00
abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027244b
更新日期：2003-05-27 00:00:00
abstract：:Incubation of 3-(2-phenylethyl)-4-methylsydnone (PMS) with liver microsomes from phenobarbital-pretreated rats or with reconstituted cytochrome P-450b results in loss of the enzyme chromophore. Chromophore loss is NADPH-dependent even though the sydnone decomposes by an oxygen- but not enzyme-dependent process to give...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00413a034
更新日期：1988-06-28 00:00:00
abstract：:Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex G-200 and hydrophobic affinity chromatograp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00591a014
更新日期：1979-11-27 00:00:00
abstract：:Retinal membrane guanylyl cyclase (RetGC) in the outer segments of vertebrate photoreceptors is controlled by guanylyl cyclase activating proteins (GCAPs), responding to light-dependent changes of the intracellular Ca(2+) concentrations. We present evidence that a different RetGC binding protein, retinal degeneration ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201342b
更新日期：2011-11-08 00:00:00
abstract：:Electron spin resonance (ESR) spectroscopy using spin-labeled ATP was used to study nucleotide binding to and structural transitions within the multidrug resistance P-glycoprotein, P-gp. Spin-labeled ATP (SL-ATP) with the spin label attached to the ribose, was observed to be an excellent substrate analogue for P-gp. S...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0512445
更新日期：2005-10-25 00:00:00
abstract：:Chloroperoxidase is a versatile fungal heme-thiolate protein that catalyzes a variety of one-electron and two-electron oxidations. We report here that the alkylation of an essential histidine residue showed no effect on the one-electron peroxidations but inhibited two-electron oxidations. The pH profiles of different ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7022656
更新日期：2008-03-04 00:00:00
abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a013
更新日期：1995-03-28 00:00:00
abstract：:Quinone oxidoreductase 2 (QR2) purified from human red blood cells was recently shown to be a potential target of the quinoline antimalarial compounds [Graves et al., (2002) Mol. Pharmacol. 62, 1364]. QR2 catalyzes the two-electron reduction of menadione via the oxidation of N-alkylated or N-ribosylated nicotinamides....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035923w
更新日期：2004-04-20 00:00:00
abstract：:Copper binding to the human copper chaperone for superoxide dismutase (hCCS) has been investigated by X-ray absorption spectroscopy. Stoichiometry measurements on the dialyzed, as-isolated protein indicated that up to 3.5 Cu ions bound per hCCS molecule. Reduction with either sodium dithionite or dithiothreitol decrea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000690j
更新日期：2000-06-27 00:00:00
abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972827k
更新日期：1998-05-19 00:00:00
abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300704c
更新日期：2012-09-11 00:00:00
abstract：:The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this reaction by the EcoRV methyltransferase. However, in vitro, the EcoRV n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00500a003
更新日期：1990-12-04 00:00:00
abstract：:Multidimensional heteronuclear NMR techniques were applied to study the phosphotransfer domain, residues 1-134, of the histidine kinase CheA, from Escherichia coli, which contains the site of autophosphorylation, His48. Assignments of the backbone amide groups and side chain protons are nearly complete. Our studies sh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00042a018
更新日期：1995-10-24 00:00:00
abstract：:The helical structure of double-stranded DNA is destabilized by increasing temperature. Above a critical temperature (the melting temperature), the two strands in duplex DNA become fully separated. Below this temperature, the structural effects are localized. Using tethered particle motion in a temperature-controlled ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500344j
更新日期：2014-10-21 00:00:00
abstract：:NMR studies of the adenosine analog tubercidin have been carried out in the presence of Escherichia coli purine nucleoside phosphorylase (PNP) in order to characterize the conformation of the enzyme-complexed nucleoside. Although analysis of transferred NOE data at various enzyme/inhibitor ratios indicated a predomina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00190a007
更新日期：1994-06-21 00:00:00
abstract：:Quantum tunneling and protein dynamics have emerged as important components of enzyme function. This review focuses on soybean lipoxygenase-1, to illustrate how the properties of enzymatic C-H bond activation link protein motions to the fundamental bond making-breaking processes. ...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi301504m
更新日期：2013-03-26 00:00:00
abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a048
更新日期：1989-01-10 00:00:00
abstract：:The inducible nitric oxide synthase core oxygen domain (iNOS(COD)) is a homodimeric protein complex of ca. 100 kDa. In this work, the subunit disassembly and unfolding of the protein following a pH jump from 7.5 to 2.8 were monitored by on-line rapid mixing in conjunction with electrospray (ESI) time-of-flight mass sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047684y
更新日期：2005-02-22 00:00:00
abstract：:The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001276m
更新日期：2000-10-17 00:00:00
abstract：:The stereoselectivity of the adenosine cyclic 3',5'-phosphate (cAMP) binding sites on the regulatory subunit of the type II bovine cardiac muscle cAMP-dependent protein kinase was investigated by examining the interactions of (Rp)- and (Sp)-adenosine cyclic 3',5'-phosphorothioates (cAMPS) with these sites. While activ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00261a028
更新日期：1982-08-31 00:00:00
abstract：:The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00540
更新日期：2018-07-31 00:00:00
