Abstract:
:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic promoter containing two estrogen response elements (EREs). To examine transcriptional synergism by XER and by XER160/586, we determined the activity of promoters containing EREs and binding sites for either the vitellogenin activator, NF1, or AP1 upstream activator protein. For the three promoters transcription by XER was 2.8-fold greater than expected for additive activities, and transcription by XER160/586 was 6.2-fold greater. These data demonstrate that an upstream activator protein bound near the promoter can partially compensate for the loss of the internal N-terminal (AF1) transactivation domain in XER160/586. Using a promoter interference assay to study the intracellular interaction between ER and the estrogen response element, we found that XER160/586 exhibited a significant increase in affinity for the ERE. Its low basal activity and enhanced affinity for the ERE make XER160/586 an effective dominant negative mutant. When co-expressed with wild-type XER at 1:1 and 5:1 ratios, XER160/586 suppressed the activity of wild-type XER by 57% and > 80%, respectively.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Xing H,Mattick S,Lew D,Shapiro DJdoi
10.1021/bi00012a013subject
Has Abstractpub_date
1995-03-28 00:00:00pages
3956-63issue
12eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700954e
更新日期:2007-11-27 00:00:00
abstract::The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9910880
更新日期:1999-09-14 00:00:00
abstract::The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100 degrees C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and 1H NMR, the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00393a042
更新日期:1987-09-22 00:00:00
abstract::We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the protein...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963174v
更新日期:1997-05-27 00:00:00
abstract::A high molecular weight acyl coenzyme A (acyl-CoA) thioesterase, designated thioesterase II, has been purified 5300-fold from photoheterotrophically grown cells of Rhodopseudomonas sphaeroides. In contrast to R. sphaeroides acyl-CoA thioesterase I [Boyce, S.G., & Lueking, D.R. (1984) Biochemistry 23, 141-147], thioest...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00357a029
更新日期:1986-05-06 00:00:00
abstract::The characteristic period four oscillation patterns of oxygen evolution induced by a train of single-turnover flashes were measured as a function of temperature in dark-adapted photosystem II (PS II) membrane fragments that were reconstituted with native plastoquinone-9 (PQ-9) by a recently developed procedure [Kurrec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962302c
更新日期:1997-03-04 00:00:00
abstract::We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a045
更新日期:1986-05-20 00:00:00
abstract::Alginate is a heteropolysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G). The Gram-negative bacterium Sphingomonas sp. A1 directly incorporates alginate into the cytoplasm through the periplasmic solute-binding protein (AlgQ1 and AlgQ2)-dependent ABC transporter (AlgM1-AlgM2/AlgS-AlgS). Two bindi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300194f
更新日期:2012-05-01 00:00:00
abstract::The Escherichia coli ATP-consuming chaperonin machinery, a complex between GroEL and GroES, has evolved to facilitate folding of substrate proteins (SPs) that cannot do so spontaneously. A series of kinetic experiments show that the SPs are encapsulated in the GroEL/ES nanocage for a short duration. If confinement of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00903
更新日期:2021-01-19 00:00:00
abstract::Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100728q
更新日期:2010-07-27 00:00:00
abstract::We have investigated the formation of the aa-tRNA X EF-Tu X GTP ternary complex spectroscopically by monitoring a fluorescence change that accompanies the association of EF-Tu X GTP with Phe-tRNAPhe-F8, a functionally active analogue of Phe-tRNAPhe with a fluorescein moiety covalently attached to the s4U-8 base. With ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00324a023
更新日期:1985-01-29 00:00:00
abstract::To elucidate the role of the proline residue in the engineered signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae, we have remodeled an idealized signal sequence L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [Yamamoto, Y., Taniyama, Y., Kikuchi, M., & Ikehara, M. (1987) Biochem. Bio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00432a054
更新日期:1989-03-21 00:00:00
abstract::A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00441a052
更新日期:1989-07-25 00:00:00
abstract::Trypanosoma brucei ornithine decarboxylase was reconstituted by coexpression of two polypeptides corresponding to residues 1-305 and residues 306-425 in Escherichia coli. The two peptides were coexpressed, at wild-type levels, from a single transcriptional unit that was separated by a 15-nucleotide untranslated region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a021
更新日期:1995-10-17 00:00:00
abstract::The insulin hexamer is an allosteric protein widely used in formulations for the treatment of diabetes. The hexamer exhibits positive and negative cooperativity and apparent half-site binding activity, reflecting the interconversion of three allosteric states, designated as T6, T3R3, and R6. The hexamer contains two s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050392s
更新日期:2005-05-31 00:00:00
abstract::The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035271b
更新日期:2003-12-16 00:00:00
abstract::The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9018626
更新日期:2010-02-02 00:00:00
abstract::Excluded volume and viscosity effects of crowding agents that mimic crowded conditions in vivo on "classical" burst phase folding kinetics of cytochrome c are assessed in vitro. Upon electron transfer-triggered folding of reduced cytochrome c, far-UV time-resolved circular dichroism (TRCD) is used to monitor folding u...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301324y
更新日期:2012-12-11 00:00:00
abstract::Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00790
更新日期:2015-10-20 00:00:00
abstract::Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for leukocyte transendothelia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300925r
更新日期:2012-10-16 00:00:00
abstract::Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a007
更新日期:1979-09-18 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) dependent urocanase (4'-imidazolone-5'-propionate hydro-lyase, EC 4.2.1.49) from Pseudomonas putida was found to catalyze an exchange reaction between solvent and the 4'-hydrogen of urocanate or imidazolepropionate at a rate faster than that of overall deuterium was compared to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00504a022
更新日期:1981-01-06 00:00:00
abstract::Aminopeptidase B (APB, EC 3.4.11.6) preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00964
更新日期:2015-10-06 00:00:00
abstract::A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002005e
更新日期:2001-05-08 00:00:00
abstract::Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970356w
更新日期:1997-10-07 00:00:00
abstract::The interaction of profilin and non-muscle beta,gamma-actin prepared from bovine spleen has been investigated under physiologic ionic conditions. Profilin binding to actin decreases the affinity of actin for MgADP and MgATP by about 65- and 13-fold, respectively. Kinetic measurements indicate that profilin binding to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001520+
更新日期:2000-10-31 00:00:00
abstract::Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9008402
更新日期:2009-09-08 00:00:00
abstract::In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00207a015
更新日期:1994-10-18 00:00:00
abstract::Electron paramagnetic resonance (EPR) measurements were performed on photosystem II (PSII) membranes that were treated with 2 M NaCl to release the 17- and 23-kilodalton (kDa) polypeptides. By using 75 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea to limit the photosystem II samples to one stable charge separation in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00369a022
更新日期:1986-10-21 00:00:00
abstract::The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035372+
更新日期:2003-11-04 00:00:00