Abstract:
:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to enzyme modulation and the controversial phenomena of direct NADH channeling. In the present paper, we reproduce with additional controls the experiments described by Yong et al. ((1993) Biochemistry 32, 11124-11131). Our results conclusively demonstrate the absence of detectable association between alpha-glycol-3-phosphate dehydrogenase and l-lactate dehydrogenase.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HOdoi
10.1021/bi027244bsubject
Has Abstractpub_date
2003-05-27 00:00:00pages
6259-63issue
20eissn
0006-2960issn
1520-4995journal_volume
42pub_type
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