Absence of evidence for metabolite-modulated association between alpha-glycerol-3-phosphate dehydrogenase and L-lactate dehydrogenase.

Abstract:

:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to enzyme modulation and the controversial phenomena of direct NADH channeling. In the present paper, we reproduce with additional controls the experiments described by Yong et al. ((1993) Biochemistry 32, 11124-11131). Our results conclusively demonstrate the absence of detectable association between alpha-glycol-3-phosphate dehydrogenase and l-lactate dehydrogenase.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

doi

10.1021/bi027244b

subject

Has Abstract

pub_date

2003-05-27 00:00:00

pages

6259-63

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

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