Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein.

Abstract:

:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars, but not by synthetic RGD-containing peptide or sugars not related to galactose. All three proteins incorporated radiolabel upon extracellular iodination and contained [3H]leucine following metabolic labeling, confirming that each is a synthetic product of the cell. The 67-kDa protein could be released from the cell surface with lactose-containing buffers, whereas solubilization of the 61- and 55-kDa components required the presence of detergent. Although all three proteins were retained on elastin affinity columns, the 61- and 55-kDa components were retained only in the presence of 67-kDa protein, suggesting that the 67-kDa protein binds elastin and the 61- and 55-kDa proteins bind to the 67-kDa protein. We propose that the 67-, 61-, and 55-kDa proteins constitute an elastin-receptor complex that forms a transmembrane link between the extracellular matrix and the intracellular compartment.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

doi

10.1021/bi00435a014

subject

Has Abstract

pub_date

1989-05-02 00:00:00

pages

3716-22

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

28

pub_type

杂志文章
  • Thermodynamic characterization of single mismatches found in naturally occurring RNA.

    abstract::Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701311c

    authors: Davis AR,Znosko BM

    更新日期:2007-11-20 00:00:00

  • Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics.

    abstract::Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi047773a

    authors: Su X,Han X,Mancuso DJ,Abendschein DR,Gross RW

    更新日期:2005-04-05 00:00:00

  • Characterization of Lck-binding elements in the herpesviral regulatory Tip protein.

    abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0485068

    authors: Bauer F,Hofinger E,Hoffmann S,Rösch P,Schweimer K,Sticht H

    更新日期:2004-11-30 00:00:00

  • "Nonclassical" secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane.

    abstract::Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0355239

    authors: Danielsen EM,van Deurs B,Hansen GH

    更新日期:2003-12-16 00:00:00

  • Modification of the conformational equilibria in the sodium and potassium dependent adenosinetriphosphatase with glutaraldehyde.

    abstract::Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00288a016

    authors: Chipman DM,Lev A

    更新日期:1983-09-13 00:00:00

  • Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase.

    abstract::Catalase-peroxidases (KatGs) are unique bifunctional heme peroxidases with an additional posttranslationally formed redox-active Met-Tyr-Trp cofactor that is essential for catalase activity. On the basis of studies of bacterial KatGs, controversial mechanisms of hydrogen peroxide oxidation were proposed. The recent di...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00436

    authors: Gasselhuber B,Graf MM,Jakopitsch C,Zamocky M,Nicolussi A,Furtmüller PG,Oostenbrink C,Carpena X,Obinger C

    更新日期:2016-06-28 00:00:00

  • X-ray crystallographic and analytical ultracentrifugation analyses of truncated and full-length yeast copper chaperones for SOD (LYS7): a dimer-dimer model of LYS7-SOD association and copper delivery.

    abstract::Copper-zinc superoxide dismutase (CuZnSOD) acquires its catalytic copper ion through interaction with another polypeptide termed the copper chaperone for SOD. Here, we combine X-ray crystallographic and analytical ultracentrifugation methods to characterize rigorously both truncated and full-length forms of apo-LYS7, ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi992716g

    authors: Hall LT,Sanchez RJ,Holloway SP,Zhu H,Stine JE,Lyons TJ,Demeler B,Schirf V,Hansen JC,Nersissian AM,Valentine JS,Hart PJ

    更新日期:2000-04-04 00:00:00

  • Structural studies of lysyl-tRNA synthetase: conformational changes induced by substrate binding.

    abstract::Lysyl-tRNA synthetase is a member of the class II aminoacyl-tRNA synthetases and catalyses the specific aminoacylation of tRNA(Lys). The crystal structure of the constitutive lysyl-tRNA synthetase (LysS) from Escherichia coli has been determined to 2.7 A resolution in the unliganded form and in a complex with the lysi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001487r

    authors: Onesti S,Desogus G,Brevet A,Chen J,Plateau P,Blanquet S,Brick P

    更新日期:2000-10-24 00:00:00

  • Structural characterization of two interchangeable conformations of a 2-aminofluorene-modified DNA oligomer by NMR and energy minimization.

    abstract::One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00250a012

    authors: Eckel LM,Krugh TR

    更新日期:1994-11-22 00:00:00

  • Exploring the role and the binding affinity of a second zinc equivalent in B. cereus metallo-beta-lactamase.

    abstract::Metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. Enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. We have investigated the effe...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010933n

    authors: Rasia RM,Vila AJ

    更新日期:2002-02-12 00:00:00

  • Effect of deoxyribonucleic acid on the production of reduced oxygen by bleomycin and iron.

    abstract::The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00531a021

    authors: Caspary WJ,Lanzo DA,Niziak C

    更新日期:1982-01-19 00:00:00

  • Engineering of fibroblast growth factor: alteration of receptor binding specificity.

    abstract::A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00003a004

    authors: Seddon AP,Aviezer D,Li LY,Böhlen P,Yayon A

    更新日期:1995-01-24 00:00:00

  • Effect of monoclonal antibody binding on alpha-beta gamma subunit interactions in the rod outer segment G protein, Gt.

    abstract::The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00451a047

    authors: Mazzoni MR,Hamm HE

    更新日期:1989-12-12 00:00:00

  • Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.

    abstract::In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi801831r

    authors: Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

    更新日期:2009-02-03 00:00:00

  • Effect of salt and pH on the reductive half-reaction of Mycobacterium tuberculosis FprA with NADPH.

    abstract::Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi702250h

    authors: Pennati A,Zanetti G,Aliverti A,Gadda G

    更新日期:2008-03-18 00:00:00

  • Toward engineering the stability and hemin-binding properties of microsomal cytochromes b5 into rat outer mitochondrial membrane cytochrome b5: examining the influence of residues 25 and 71.

    abstract::As part of a larger effort to engineer the stability and hemin-binding properties of microsomal (Mc) cytochromes b(5) into rat liver outer mitochondrial membrane (OM) cytochrome (cyt) b(5), several mutants of rat OM cyt b(5) were prepared to study the effect of gradual and complete elimination of two extended hydropho...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi026005l

    authors: Cowley AB,Altuve A,Kuchment O,Terzyan S,Zhang X,Rivera M,Benson DR

    更新日期:2002-10-01 00:00:00

  • Electron/proton coupling in bacterial nitric oxide reductase during reduction of oxygen.

    abstract::The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050524h

    authors: Flock U,Watmough NJ,Adelroth P

    更新日期:2005-08-09 00:00:00

  • Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis.

    abstract::We have used site-directed mutagenesis to alter the [Fe-S] cluster composition of Escherichia coli dimethyl sulfoxide (DMSO) reductase (DmsABC). The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [Cammack, R., & Weiner, ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00098a003

    authors: Rothery RA,Weiner JH

    更新日期:1991-08-27 00:00:00

  • Inhibition of ribosomal translocation by peptidyl transfer ribonucleic acid analogues.

    abstract::The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00270a013

    authors: Wagner T,Sprinzl M

    更新日期:1983-01-04 00:00:00

  • Cloning and expression of goldfish opsin sequences.

    abstract::Five opsin cDNA clones were isolated from a goldfish retina cDNA library and sequenced. On the basis of homology to previously characterized visual pigments, one clone was identified as goldfish rod opsin and a second as a goldfish red cone opsin. Two rhodopsin-like clones were found to be similar to the chicken green...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00052a027

    authors: Johnson RL,Grant KB,Zankel TC,Boehm MF,Merbs SL,Nathans J,Nakanishi K

    更新日期:1993-01-12 00:00:00

  • Nucleotide- and substrate-induced conformational transitions in the CBS domain-containing pyrophosphatase of Moorella thermoacetica.

    abstract::In contrast to all other known pyrophosphatases, Moorella thermoacetica pyrophosphatase (mtCBS-PPase) contains nucleotide-binding CBS domains and is thus strongly regulated by adenine nucleotides. Stopped-flow measurements using a fluorescent AMP analogue, 2'(3')-O-(N-methylanthranoyl)-AMP (Mant-AMP), reveal that nucl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9019737

    authors: Jämsen J,Baykov AA,Lahti R

    更新日期:2010-02-09 00:00:00

  • Anticoagulantly active heparin-like molecules from vascular tissue.

    abstract::Mucopolysaccharides were isolated from calf cerebral microvasculature and calf aorta. The only complex carbohydrates that exhibited anticoagulant activity were heparin-like components. The biologic potencies of calf cerebral and aortic heparin-like species were 2.92 units/mg of anti-factor Xa activity and 2.85 units/m...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00303a023

    authors: Marcum JA,Rosenberg RD

    更新日期:1984-04-10 00:00:00

  • Quantification of quaternary structure stability in aggregation-prone proteins under physiological conditions: the transthyretin case.

    abstract::The quaternary structure stability of proteins is typically studied under conditions that accelerate their aggregation/unfolding processes on convenient laboratory time scales. Such conditions include high temperature or pressure, chaotrope-mediated unfolding, or low or high pH. These approaches have the limitation of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500739q

    authors: Robinson LZ,Reixach N

    更新日期:2014-10-21 00:00:00

  • A novel substrate mimetic inhibitor of PKB/Akt inhibits prostate cancer tumor growth in mice by blocking the PKB pathway.

    abstract::We describe a novel, potent peptide substrate mimetic inhibitor of protein kinase B (PKB/Akt). The compound selectively kills prostate cancer cells, in which PKB is highly activated, but not normal cells, or cancer cells in which PKB is not activated. The inhibitor induces apoptosis and inhibits the phosphorylation of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061928s

    authors: Litman P,Ohne O,Ben-Yaakov S,Shemesh-Darvish L,Yechezkel T,Salitra Y,Rubnov S,Cohen I,Senderowitz H,Kidron D,Livnah O,Levitzki A,Livnah N

    更新日期:2007-04-24 00:00:00

  • Deletion of the Mitochondrial Protein VWA8 Induces Oxidative Stress and an HNF4α Compensatory Response in Hepatocytes.

    abstract::von Willebrand A domain-containing protein 8 (VWA8) is a poorly characterized, mitochondrial matrix-targeted protein with an AAA ATPase domain and ATPase activity that increases in livers of mice fed a high-fat diet. This study was undertaken to use CRISPR/Cas9 to delete VWA8 in cultured mouse hepatocytes and gain ins...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.9b00863

    authors: Luo M,Willis WT,Coletta DK,Langlais PR,Mengos A,Ma W,Finlayson J,Wagner GR,Shi CX,Mandarino LJ

    更新日期:2019-12-10 00:00:00

  • Specific inhibition of expression of a human collagen gene (COL1A1) with modified antisense oligonucleotides. The most effective target sites are clustered in double-stranded regions of the predicted secondary structure for the mRNA.

    abstract::A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00202a024

    authors: Laptev AV,Lu Z,Colige A,Prockop DJ

    更新日期:1994-09-13 00:00:00

  • Hydron transfer catalyzed by triosephosphate isomerase. Products of the direct and phosphite-activated isomerization of [1-(13)C]-glycolaldehyde in D(2)O.

    abstract::Product distributions for the reaction of glycolaldehyde labeled with carbon-13 at the carbonyl carbon ([1-(13)C]-GA) catalyzed by triosephosphate isomerase (TIM) in D(2)O at pD 7.0 in the presence of phosphite dianion and in its absence were determined by (1)H NMR spectroscopy. We observe three products for the relat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900636c

    authors: Go MK,Amyes TL,Richard JP

    更新日期:2009-06-23 00:00:00

  • Purification and functional characterization of phiX174 lysis protein E.

    abstract::Two classes of bacteriophages, the single-stranded DNA Microviridae and the single-stranded RNA Alloleviviridae, accomplish lysis by expressing "protein antibiotics", or polypeptides that inhibit cell wall biosynthesis. Previously, we have provided genetic and physiological evidence that E, a 91-amino acid membrane pr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900469g

    authors: Zheng Y,Struck DK,Young R

    更新日期:2009-06-09 00:00:00

  • Extended X-ray absorption fine structure of copper in CuA-depleted, p-(hydroxymercuri)benzoate-modified, and native cytochrome c oxidase.

    abstract::Cytochrome c oxidase contains four redox-active metal centers: two heme irons, cytochromes a and a3, and two copper ions, CuA and CuB. Due to the paucity of spectroscopic signatures for both copper sites in cytochrome c oxidase, the ligands and structures for these sites have remained ambiguous. The specific depletion...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00382a005

    authors: Li PM,Gelles J,Chan SI,Sullivan RJ,Scott RA

    更新日期:1987-04-21 00:00:00

  • Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase Lck.

    abstract::Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi015986j

    authors: Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

    更新日期:2002-04-23 00:00:00