Abstract：
:Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% as estimated by fluorescence induction and LHCII/PSII stoichiometry. These low-light-induced changes in the protein composition were accompanied by the formation of ordered particle arrays in the exoplasmic fracture face in grana thylakoids detected by freeze-fracture electron microscopy. Most likely these highly ordered arrays consist of PSII complexes. A statistical analysis of the particles in these structures shows that the distance of neighboring complexes in the same row is 18.0 nm, the separation between two rows is 23.7 nm, and the angle between the particle axis and the row is 26 degrees . On the basis of structural information on the photosystem II supercomplex, a model on the supramolecular arrangement was generated predicting that two neighboring complexes share a trimeric light-harvesting complex. It was suggested that the supramolecular reorganization in ordered arrays in low-light grana thylakoids is a strategy to overcome potential diffusion problems in this crowded membrane. Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kirchhoff H,Haase W,Wegner S,Danielsson R,Ackermann R,Albertsson PAdoi
10.1021/bi700748ysubject
Has Abstractpub_date
2007-10-02 00:00:00pages
11169-76issue
39eissn
0006-2960issn
1520-4995journal_volume
46pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Raman spectra of phycocyanobilin, phycocyanin, and allophycocyanin were obtained at resonance with their visible and near-UV transitions. These spectra were empirically assigned with the help of 14N- and 15N-isotopic substitutions and comparisons with resonance Raman spectra of phycoerythrin. These results confirm the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00205a019
更新日期：1994-10-04 00:00:00
abstract：:To determine whether ligand-receptor interactions could engender the selective uptake by deficient cells of enzyme-laden liposomes, aggregated human IgG was used to coat liposomes which had previously trapped purified hexosaminidase A (Hex A). By a new, high-yield procedure, Hex A was purified 7000-fold from human pla...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00647a034
更新日期：1976-01-27 00:00:00
abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00126a014
更新日期：1992-03-24 00:00:00
abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00508a031
更新日期：1981-03-03 00:00:00
abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a002
更新日期：1990-03-13 00:00:00
abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9006786
更新日期：2009-08-25 00:00:00
abstract：:Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0525977
更新日期：2006-04-11 00:00:00
abstract：:The nuclease hypersensitivity element III(1) (NHE III(1)) of the c-MYC promoter strongly controls the transcriptional activity of the c-MYC oncogene. The purine-rich strand of the NHE III(1) element has been shown to be a silencer element for c-MYC transcription upon formation of a G-quadruplex structure. We have dete...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048242p
更新日期：2005-02-15 00:00:00
abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9725069
更新日期：1998-05-12 00:00:00
abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001068u
更新日期：2000-10-17 00:00:00
abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00453a003
更新日期：1990-01-09 00:00:00
abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700726a
更新日期：2007-10-23 00:00:00
abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a030
更新日期：1975-09-23 00:00:00
abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00334a003
更新日期：1985-06-18 00:00:00
abstract：:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00347a019
更新日期：1985-12-17 00:00:00
abstract：:The proprotein convertase furin is a highly specific serine protease modifying and thereby activating proteins in the secretory pathway by proteolytic cleavage. Its substrates are involved in many diseases, including cancer and infections caused by bacteria and viruses. Understanding furin's substrate specificity is c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01124
更新日期：2018-02-13 00:00:00
abstract：:An attractive strategy for the development of anti-retroviral drugs is the exploration of compounds that mimic RNA control regions of the viral genome and act as "decoys" to sequester viral gene regulatory proteins. Decoys consisting of RNA, however, are chemically unstable and readily degraded by cellular nucleases. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961980w
更新日期：1997-01-21 00:00:00
abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00430a033
更新日期：1989-02-21 00:00:00
abstract：:Previous studies of titratable (Na+ + K+)-ATPase sulfhydryl groups have indicated the presence of one disulfide bond per mole of holoenzyme. This single disulfide cross-link was assigned to the beta subunit on the basis of the difference between the number of titrated "free" sulfhydryl groups and the total number of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a025
更新日期：1990-02-13 00:00:00
abstract：:The rate of deuterium exchange of a purine 8CH group in DNA is highly sensitive to both macromolecular secondary structure and intermolecular interactions which restrict solvent access to the major groove [Lamba, O.P., Becka, R., & Thomas, G.J., Jr. (1990) Biopolymers 29, 1465-1477]. We have exploited the sensitivity ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00127a012
更新日期：1992-03-31 00:00:00
abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200595z
更新日期：2011-06-14 00:00:00
abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049206t
更新日期：2005-02-15 00:00:00
abstract：:Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052216p
更新日期：2006-04-18 00:00:00
abstract：:The ATP binding cassette (ABC) transporters ABCG2 and ABCB1 perform ATP hydrolysis-dependent efflux of structurally highly diverse compounds, collectively called allocrites. Whereas much is known about allocrite-ABCB1 interactions, the chemical nature and strength of ABCG2-allocrite interactions have not yet been asse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00649
更新日期：2015-10-13 00:00:00
abstract：:Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00174a021
更新日期：1994-03-01 00:00:00
abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962139l
更新日期：1997-03-11 00:00:00
abstract：:Apolipoprotein (apo) A-I, a 243-residue, 28.1-kDa protein is a major mediator of the reverse cholesterol transport (RCT) pathway, a process that may reduce the risk of cardiovascular disease in humans. In plasma, a small fraction of lipid-free or lipid-poor apoA-I is likely a key player in the first step of RCT. There...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047717+
更新日期：2005-03-01 00:00:00
abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992429c
更新日期：2000-05-09 00:00:00
abstract：:Cytochrome c oxidase contains four redox-active metal centers: two heme irons, cytochromes a and a3, and two copper ions, CuA and CuB. Due to the paucity of spectroscopic signatures for both copper sites in cytochrome c oxidase, the ligands and structures for these sites have remained ambiguous. The specific depletion...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00382a005
更新日期：1987-04-21 00:00:00
abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00544a019
更新日期：1980-02-05 00:00:00
