Stability effects of increasing the hydrophobicity of solvent-exposed side chains in staphylococcal nuclease.

abstract：:The kinetics and energetics of the binding between barley alpha-amylase/subtilisin inhibitor (BASI) or BASI mutants and barley alpha-amylase 2 (AMY2) were determined using surface plasmon resonance and isothermal titration calorimetry (ITC). Binding kinetics were in accordance with a 1:1 binding model. At pH 5.5, [Ca(...

journal_title：Biochemistry

authors： Nielsen PK,Bønsager BC,Berland CR,Sigurskjold BW,Svensson B

更新日期：2003-02-18 00:00:00

abstract：:Biofilms are surface-attached communities of bacteria enclosed in a polysaccharide matrix. Bacteria in a biofilm are extremely resistant to antibiotics. Several recent reports have linked the signaling molecule nitric oxide (NO) with biofilm dispersal. We have previously reported that an H-NOX (heme-nitric oxide/oxyge...

journal_title：Biochemistry

authors： Lahiri T,Luan B,Raleigh DP,Boon EM

更新日期：2014-04-08 00:00:00

abstract：:The DNA binding of three platinum(II) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. In 0.2 M NaCl, binding data for calf thymus DNA show the association constants to be approximately 10(4) M-1. The bindin...

journal_title：Biochemistry

authors： Howe-Grant M,Lippard SJ

更新日期：1979-12-25 00:00:00

abstract：:We have studied alkylation of the acetylcholine receptor by N-[3H]ethylmaleimide ([3H]NEM) under various conditions. The radiolabeled preparations were submitted to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to separate the receptor complex into subunits, and the incorporation of 3H into each type of ch...

journal_title：Biochemistry

authors： de Souza Otero A,Hamilton SL

更新日期：1984-05-22 00:00:00

abstract：:Nonsense mutations that result in premature stop codons in the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to the lack of β-globin. Compounds that induce translational readthrough across the thalassemia-causing premature stop codon will have therapeutic benefits. Curre...

journal_title：Biochemistry

authors： Kar D,Sellamuthu K,Kumar SD,Eswarappa SM

更新日期：2020-01-14 00:00:00

abstract：:Acyl-CoA:cholesterol O-acyltransferase (EC 2.3.1.26) (ACAT) catalyzes the intracellular synthesis of cholesteryl esters from cholesterol and fatty acyl-CoA at neutral pH. Despite the probable pathophysiologic role of ACAT in vascular cholesteryl ester accumulation during atherogenesis, its mechanism of action and its ...

journal_title：Biochemistry

authors： Kinnunen PM,DeMichele A,Lange LG

更新日期：1988-09-20 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...

journal_title：Biochemistry

authors： Yang XY,Schulz H,Elzinga M,Yang SY

更新日期：1991-07-09 00:00:00

abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...

journal_title：Biochemistry

authors： Jackson RC

更新日期：1976-12-14 00:00:00

abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...

journal_title：Biochemistry

authors： Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

更新日期：1994-04-05 00:00:00

abstract：:The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...

journal_title：Biochemistry

authors： Sackett DL,Lippoldt RE

更新日期：1991-04-09 00:00:00

abstract：:The sterol (free and esterified cholesterol) transport activity of the small-intestinal brush border membrane was solubilized with the short-chain detergent diheptanoylphosphatidylcholine and reconstituted to an artificial membrane system (proteoliposomes). The resulting proteoliposomes were identified as unilamellar ...

journal_title：Biochemistry

authors： Boffelli D,Weber FE,Compassi S,Werder M,Schulthess G,Hauser H

更新日期：1997-09-02 00:00:00

abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...

journal_title：Biochemistry

authors： Darfeuille F,Arzumanov A,Gait MJ,Di Primo C,Toulmé JJ

更新日期：2002-10-08 00:00:00

abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

journal_title：Biochemistry

authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

更新日期：1998-05-19 00:00:00

abstract：:The active site of water oxidation in Photosystem II (PSII) is a Mn4CaO5 cluster that is located in a cavity between the D1 and CP43 protein subunits by which it is coordinated. The remainder of this cavity is filled with water molecules, which serve as a source of substrate and participate in poorly understood hydrog...

journal_title：Biochemistry

authors： Dilbeck PL,Bao H,Neveu CL,Burnap RL

更新日期：2013-10-01 00:00:00

abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

journal_title：Biochemistry

authors： Riccardi D,König P,Guo H,Cui Q

更新日期：2008-02-26 00:00:00

abstract：:The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...

journal_title：Biochemistry

authors： Dhawan IK,Shelver D,Thorsteinsson MV,Roberts GP,Johnson MK

更新日期：1999-09-28 00:00:00

abstract：:Soluble guanylate cyclase (sGC) is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. sGC is activated by nitric oxide (NO) and therefore plays a central role in NO signal transduction. Activation of sGC by NO is believed to be mediated by the interaction between NO and the heme of sGC. Spectroscopic a...

journal_title：Biochemistry

authors： Zhao Y,Marletta MA

更新日期：1997-12-16 00:00:00

abstract：:The origin of the nuclear magnetic resonance (NMR)-measurable ATP in equilibrium Pi exchange and whether it can be used to determine net oxidative ATP synthesis rates in the intact myocardium were examined by detailed measurements of ATP in equilibrium Pi exchange rates in both directions as a function of the myocardi...

journal_title：Biochemistry

authors： Kingsley-Hickman PB,Sako EY,Mohanakrishnan P,Robitaille PM,From AH,Foker JE,Uğurbil K

更新日期：1987-11-17 00:00:00

abstract：:Beta-lactams exert their antibiotic action through their inhibition of bacterial DD-peptidases (penicillin-binding proteins). Bacteria, in general, carry several such enzymes localized on the outside of their cell membrane to catalyze the final step in cell wall (peptidoglycan) synthesis. They have been classified int...

journal_title：Biochemistry

authors： Josephine HR,Charlier P,Davies C,Nicholas RA,Pratt RF

更新日期：2006-12-26 00:00:00

abstract：:The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted...

journal_title：Biochemistry

authors： Hjörleifsson JG,Ásgeirsson B

更新日期：2017-09-26 00:00:00

abstract：:An attractive strategy for the development of anti-retroviral drugs is the exploration of compounds that mimic RNA control regions of the viral genome and act as "decoys" to sequester viral gene regulatory proteins. Decoys consisting of RNA, however, are chemically unstable and readily degraded by cellular nucleases. ...

journal_title：Biochemistry

authors： Rigl CT,Lloyd DH,Tsou DS,Gryaznov SM,Wilson WD

更新日期：1997-01-21 00:00:00

abstract：:The ATP binding cassette (ABC) transporters ABCG2 and ABCB1 perform ATP hydrolysis-dependent efflux of structurally highly diverse compounds, collectively called allocrites. Whereas much is known about allocrite-ABCB1 interactions, the chemical nature and strength of ABCG2-allocrite interactions have not yet been asse...

journal_title：Biochemistry

authors： Xu Y,Egido E,Li-Blatter X,Müller R,Merino G,Bernèche S,Seelig A

更新日期：2015-10-13 00:00:00

abstract：:Multivalent binding is an efficient means to enhance the affinity and specificity of chemical probes targeting multidomain proteins in order to study their function and role in disease. While the theory of multivalent binding is straightforward, physical and structural characterization of bivalent binding encounters m...

journal_title：Biochemistry

authors： Suh JL,Watts B,Stuckey JI,Norris-Drouin JL,Cholensky SH,Dickson BM,An Y,Mathea S,Salah E,Knapp S,Khan A,Adams AT,Strahl BD,Sagum CA,Bedford MT,James LI,Kireev DB,Frye SV

更新日期：2018-04-10 00:00:00

abstract：:Many biological processes are now known to be regulated by Ca2+ via calmodulin (CM). Although a general mechanistic model by which Ca2+ and calmodulin modulate many of these activities has been proposed, an accurate quantitative model is not available. A detailed analysis of skeletal muscle myosin light chain kinase a...

journal_title：Biochemistry

authors： Blumenthal DK,Stull JT

更新日期：1980-11-25 00:00:00

abstract：:The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...

journal_title：Biochemistry

authors： Herold S,Exner M,Nauser T

更新日期：2001-03-20 00:00:00

abstract：:The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...

journal_title：Biochemistry

authors： Williams DL Jr,Greene LE

更新日期：1983-05-24 00:00:00

abstract：:The binding of the benzo[a]pyrene metabolite anti-BPDE (r7, t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) to the N(2) group of 2'-deoxyguanosine residues (dG) is known to adversely affect the Michaelis-Menten primer extension kinetics catalyzed by DNA Pol I and other polymerases. In this work, the impact...

journal_title：Biochemistry

authors： Rechkoblit O,Amin S,Geacintov NE

更新日期：1999-09-07 00:00:00

abstract：:His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...

journal_title：Biochemistry

authors： Adina-Zada A,Jitrapakdee S,Wallace JC,Attwood PV

更新日期：2014-02-18 00:00:00

abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...

journal_title：Biochemistry

authors： Shimamoto N,Utiyama H

更新日期：1983-12-06 00:00:00