当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Binding of platinum(II) intercalation reagents to deoxyribnonucleic acid. Dependence on base-pair composition, nature of the intercalator, and ionic strength.

Binding of platinum(II) intercalation reagents to deoxyribnonucleic acid. Dependence on base-pair composition, nature of the intercalator, and ionic strength.

Abstract：

:The DNA binding of three platinum(II) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. In 0.2 M NaCl, binding data for calf thymus DNA show the association constants to be approximately 10(4) M-1. The binding constants decrease in the order [(o-phen)Pt(en)]2+ greater than or equal to [(terpy)Pt(HET)]+ greater than [(bipy)Pt(en)]2+. The number of available intercalation sites for the doubly charged intercalators is only 70% of the number expected from the nearest-neighbor exclusion model. Binding of [(o-phen)Pt(en)]2+ and [(terpy)Pt(HET)]+ to various DNAs depends linearly on G.C content. Both reagents exhibit essentially the same degree of G.C specificity. Intercalative binding is a function of ionic strength. Increasing the salt concentration minimizes the importance of metallointercalator charge, and extrapolation to 1 M salt reveals the intercalative abilities, as reflected in binding constants, to be equivalent for [(terpy)Pt(HET)]+ and [o-phen)Pt(en)]2+ and about 1 order of magnitude less than that of ethidium.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Howe-Grant M,Lippard SJ

doi

10.1021/bi00593a003

subject

Has Abstract

pub_date

1979-12-25 00:00:00

pages

5762-9

issue

26

eissn

0006-2960

issn

1520-4995

journal_volume

18

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Mutagenic events in Escherichia coli and mammalian cells generated in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site.

    abstract：:Comparative mutagenesis studies of N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) adducts positioned in the Nar I restriction enzyme site were performed using Escherichia coli (E. coli) and simian kidney (COS-7) cells. Oligodeoxynucleotides ((5)(')TCCTCG(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0202878

    authors： Tan X,Suzuki N,Grollman AP,Shibutani S

    更新日期：2002-12-03 00:00:00

  • Zebrafish neuroglobin is a cell-membrane-penetrating globin.

    abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800286m

    authors： Watanabe S,Wakasugi K

    更新日期：2008-05-13 00:00:00

  • The anti-amyloidogenic effect is exerted against Alzheimer's beta-amyloid fibrils in vitro by preferential and reversible binding of flavonoids to the amyloid fibril structure.

    abstract：:How various anti-amyloidogenic compounds inhibit the formation of Alzheimer's beta-amyloid fibrils (fAbeta) from amyloid beta-peptide (Abeta) and destabilize fAbeta remains poorly understood. Using spectrophotometry, spectrofluorometry, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061540x

    authors： Hirohata M,Hasegawa K,Tsutsumi-Yasuhara S,Ohhashi Y,Ookoshi T,Ono K,Yamada M,Naiki H

    更新日期：2007-02-20 00:00:00

  • Conformational changes of an exchangeable apolipoprotein, apolipophorin III from Locusta migratoria, at low pH: correlation with lipid binding.

    abstract：:Locusta migratoria apolipophorin III (apoLp-III) is a helix bundle exchangeable apolipoprotein that reversibly binds to lipoprotein surfaces. Structural reorganization of its five amphipathic alpha-helices enables the transition from the lipid-free to lipid-bound state. ApoLp-III-induced transformation of dimyristoylp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010410f

    authors： Weers PM,Kay CM,Ryan RO

    更新日期：2001-06-26 00:00:00

  • 9-cis-retinoids: biosynthesis of 9-cis-retinoic acid.

    abstract：:Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992152g

    authors： Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

    更新日期：2000-07-11 00:00:00

  • Functional role of the conserved active site proline of triosephosphate isomerase.

    abstract：:The importance of the fully conserved active site proline, Pro168, for the reaction mechanism of triosephosphate isomerase (TIM) has been investigated by studying the enzymatic and crystallographic properties of the P168A variant of trypanosomal TIM. In TIM, Pro168 follows the key catalytic residue Glu167, situated at...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061683j

    authors： Casteleijn MG,Alahuhta M,Groebel K,El-Sayed I,Augustyns K,Lambeir AM,Neubauer P,Wierenga RK

    更新日期：2006-12-26 00:00:00

  • An amino acid residue (S201) in the retinal binding pocket regulates the photoreaction pathway of phoborhodopsin.

    abstract：:Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200598r

    authors： Dai G,Zhang Y,Tamogami J,Demura M,Kamo N,Kandori H,Iwasa T

    更新日期：2011-08-23 00:00:00

  • Stacking of bacteriochlorophyll c macrocycles in chlorosome from Chlorobium limicola as revealed by intermolecular 13C magnetic-dipole correlation, X-ray diffraction, and quadrupole coupling in 25Mg NMR.

    abstract：:The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801651w

    authors： Kakitani Y,Koyama Y,Shimoikeda Y,Nakai T,Utsumi H,Shimizu T,Nagae H

    更新日期：2009-01-13 00:00:00

  • Signal amplification between Gbetagamma release and PI3Kgamma-mediated PI(3,4,5)P3 formation monitored by a fluorescent Gbetagamma biosensor protein and repetitive two component total internal reflection/fluorescence redistribution after photobleaching an

    abstract：:Phosphoinositide 3-kinase gamma (PI3Kgamma) is activated by Gbetagamma release after stimulation of Galpha i -coupled receptors, involving a recruitment of the enzyme to the plasma membrane via interaction of the regulatory subunit p101 or p87 with Gbetagamma. The receptor-mediated release of Gbetagamma was, however, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800596b

    authors： Tannert A,Voigt P,Burgold S,Tannert S,Schaefer M

    更新日期：2008-10-28 00:00:00

  • Molecular mechanism of action of β-hairpin antimicrobial peptide arenicin: oligomeric structure in dodecylphosphocholine micelles and pore formation in planar lipid bilayers.

    abstract：:The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenici...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200746t

    authors： Shenkarev ZO,Balandin SV,Trunov KI,Paramonov AS,Sukhanov SV,Barsukov LI,Arseniev AS,Ovchinnikova TV

    更新日期：2011-07-19 00:00:00

  • New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid.

    abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991888a

    authors： Greasley SE,Yamashita MM,Cai H,Benkovic SJ,Boger DL,Wilson IA

    更新日期：1999-12-21 00:00:00

  • Inhibition of in vitro transcription by a triplex-forming oligonucleotide targeted to human c-myc P2 promoter.

    abstract：:Triplex-forming oligonucleotides (TFOs) have been shown to bind in a sequence-specific manner to polypurine/polypyrimidine sequences in several human gene promoters, including the c-myc P1 promoter. TFOs have been shown to inhibit transcription in vitro and the expression of target genes in cell culture. The human c-m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00025a023

    authors： Kim HG,Miller DM

    更新日期：1995-06-27 00:00:00

  • Identification of protein side chains near the membrane-aqueous interface: a site-directed spin labeling study of KcsA.

    abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015828s

    authors： Gross A,Hubbell WL

    更新日期：2002-01-29 00:00:00

  • Structural polymorphism and dynamism in the DNA segment GATCTTCCCCCCGGAA: NMR investigations of hairpin, dumbbell, nicked duplex, parallel strands, and i-motif.

    abstract：:Structure and dynamism in a DNA segment d-GATCTTCCCCCCGGAA have been investigated by nuclear magnetic resonance (NMR) spectroscopy. At neutral pH, the molecule exists largely as a dumbbell, formed by the association of two hairpins with sticky ends. A small percentage of hairpin is also detectable. The stem of the dum...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970819y

    authors： Singh S,Patel PK,Hosur RV

    更新日期：1997-10-28 00:00:00

  • Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity.

    abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015970t

    authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

    更新日期：2002-04-16 00:00:00

  • Identification of the sites phosphorylated by cyclic AMP-dependent protein kinase on the beta 2 subunit of L-type voltage-dependent calcium channels.

    abstract：:Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990896o

    authors： Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

    更新日期：1999-08-10 00:00:00

  • Properties of the high-affinity single-stranded DNA binding state of the Escherichia coli recA protein.

    abstract：:The properties of the high-affinity single-stranded DNA (ssDNA) binding state of Escherichia coli recA protein have been studied. We find that all of the nucleoside triphosphates that are hydrolyzed by recA protein induce a high-affinity ssDNA binding state. The effect of ATP binding to recA protein was partially sepa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00404a021

    authors： Menetski JP,Varghese A,Kowalczykowski SC

    更新日期：1988-02-23 00:00:00

  • Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.

    abstract：:Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00373a001

    authors： Shull GE,Greeb J,Lingrel JB

    更新日期：1986-12-16 00:00:00

  • Accessibility and external versus intercalative binding to DNA as assessed by oxygen-induced quenching of the palladium(II)-containing cationic porphyrins Pd(T4) and Pd(tD4).

    abstract：:Studies reveal that it is possible to design a palladium(II)-containing porphyrin to bind exclusively by intercalation to double-stranded DNA while simultaneously enhancing the ability to sensitize the formation of singlet oxygen. The comparisons revolve around the cations [5,10,15,20-tetra(N-methylpyridinium-4-yl)por...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401610t

    authors： Bork MA,Gianopoulos CG,Zhang H,Fanwick PE,Choi JH,McMillin DR

    更新日期：2014-02-04 00:00:00

  • Alpha-chain domain of fibrinogen controls generation of fibrinoligase (coagulation factor XIIIa). Calcium ion regulatory aspects.

    abstract：:Fibrinogen (approximately 10(-5) M) labilizes heterologous interactions within the thrombin-modified factor XIII zymogen (i.e., XIII' = a2'b2) so that, in the time frame (ca. 10 min) of normal clotting in plasma (37 degrees C, mu = 0.15, pH 7.5), 1.5 mM Ca2+ is sufficient to cause the release of the noncatalytic b sub...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00516a016

    authors： Credo RB,Curtis CG,Lorand L

    更新日期：1981-06-23 00:00:00

  • Raman spectroscopic studies of NAD coenzymes bound to malate dehydrogenases by difference techniques.

    abstract：:We report here on the Raman spectra of NADH, 3-acetylpyridine adenine dinucleotide, APAD+, and a fragment of these molecules, adenosine 5'-diphosphate ribose (ADPR) bound to the mitochondrial (mMDH) and cytoplasmic (or soluble, sMDH) forms of malate dehydrogenase. We observe changes in the Raman spectrum of the adenos...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00100a011

    authors： Deng H,Burgner J,Callender R

    更新日期：1991-09-10 00:00:00

  • Reaction of nitric oxide with the turnover intermediates of cytochrome c oxidase: reaction pathway and functional effects.

    abstract：:The reactions of nitric oxide (NO) with the turnover intermediates of cytochrome c oxidase were investigated by combining amperometric and spectroscopic techniques. We show that the complex of nitrite with the oxidized enzyme (O) is obtained by reaction of both the "peroxy" (P) and "ferryl" (F) intermediates with stoi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000447k

    authors： Giuffrè A,Barone MC,Mastronicola D,D'Itri E,Sarti P,Brunori M

    更新日期：2000-12-19 00:00:00

  • Binding of adenosine diphosphoribosyltransferase to the termini and internal regions of linear DNAs.

    abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a050

    authors： Sastry SS,Buki KG,Kun E

    更新日期：1989-06-27 00:00:00

  • O6-methylguanine in DNA inhibits replication in vitro by human cell extracts.

    abstract：:To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00212a035

    authors： Ceccotti S,Dogliotti E,Gannon J,Karran P,Bignami M

    更新日期：1993-12-14 00:00:00

  • Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide.

    abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026778+

    authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

    更新日期：2003-02-04 00:00:00

  • N-218 MLN64, a protein with StAR-like steroidogenic activity, is folded and cleaved similarly to StAR.

    abstract：:The steroidogenic acute regulatory protein (StAR) facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN-64 are deleted, the resul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000911l

    authors： Bose HS,Whittal RM,Huang MC,Baldwin MA,Miller WL

    更新日期：2000-09-26 00:00:00

  • Crystallization and partial characterization of prenyltransferase from avian liver.

    abstract：:Prenyltransferase (EC 2.5.1.1) has been obtained from chicken liver in a stable crystalline form. The enzyme has been shown to be homogeneous by polyacrylamide gel electrophoresis at pH 8.4, and by electrophoresis in sodium dodecyl sulfate containing gels. Electrofocusing of the crystalline enzyme results in a single ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00672a009

    authors： Reed BC,Rilling HC

    更新日期：1975-01-14 00:00:00

  • Base-flipping mutations of uracil DNA glycosylase: substrate rescue using a pyrene nucleotide wedge.

    abstract：:We recently introduced a new substrate rescue tool for investigating enzymatic base flipping by uracil DNA glycosylase (UDG) in which a bulky pyrene nucleotide wedge (Y) was placed opposite a uracil in duplex DNA (i.e., a U/Y pair), thereby preorganizing the target base in an extrahelical conformation [Jiang, Y. L., e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026227j

    authors： Jiang YL,Stivers JT,Song F

    更新日期：2002-09-17 00:00:00

  • Acetylcholine receptor and ionic channel of Torpedo electroplax: binding of perhydrohistrionicotoxin to membrane and solubilized preparations.

    abstract：:The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00618a023

    authors： Eldefrawi ME,Eldefrawi AT,Mansour NA,Daly JW,Witkop B,Albuquerque EX

    更新日期：1978-12-12 00:00:00

  • Structural domains of DNA mesojunctions.

    abstract：:Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00003a026

    authors： Wang H,Seeman NC

    更新日期：1995-01-24 00:00:00