当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > O6-methylguanine in DNA inhibits replication in vitro by human cell extracts.

O6-methylguanine in DNA inhibits replication in vitro by human cell extracts.

Abstract：

:To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitrosourea concentrations that introduced an average of 0.3-2.5 O6-methylguanine and equal amounts of 3-methyladenine lesions per DNA molecule. When methylated plasmid was incubated with extract of Mex-HeLaMR cells under conditions favoring DNA replication, an impairment of replication was observed as the accumulation of incompletely replicated form II plasmid molecules. These extracts simultaneously performed a T antigen independent, DpnI-sensitive DNA repair synthesis that increased with increasing DNA damage. Subtraction of this repair DNA synthesis revealed that methylation inhibited overall replication. At low levels of methylation (< or = 1 O6-methylguanine and < or = 1 3-methyladenine lesion per plasmid), inhibition was transient, while more extensive damage resulted in apparently irreversible inhibition of replication. Removal of O6-methylguanine by pretreatment of the methylated plasmid with purified human O6-methylguanine-DNA methyltransferase restored replication to almost normal levels. When the methylated plasmid was replicated by extracts of Mex+ HeLaS3 cells proficient in the repair of O6-methylguanine, a lower level of inhibition and less repair DNA synthesis was observed. The inhibition of DNA synthesis and the stimulation of repair DNA synthesis are thus both largely due to the presence of O6-methylguanine in DNA.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ceccotti S,Dogliotti E,Gannon J,Karran P,Bignami M

doi

10.1021/bi00212a035

subject

Has Abstract

pub_date

1993-12-14 00:00:00

pages

13664-72

issue

49

eissn

0006-2960

issn

1520-4995

journal_volume

32

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Characterization of Lck-binding elements in the herpesviral regulatory Tip protein.

    abstract：:Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0485068

    authors： Bauer F,Hofinger E,Hoffmann S,Rösch P,Schweimer K,Sticht H

    更新日期：2004-11-30 00:00:00

  • Interaction of LDS-751 with P-glycoprotein and mapping of the location of the R drug binding site.

    abstract：:One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0485326

    authors： Lugo MR,Sharom FJ

    更新日期：2005-01-18 00:00:00

  • Ligand binding properties of bacterial hemoglobins and flavohemoglobins.

    abstract：:Bacterial hemoglobins and flavohemoglobins share a common globin fold but differ otherwise in structural and functional aspects. The bases of these differences were investigated through kinetic studies on oxygen, carbon monoxide, and nitric oxide binding. The novel bacterial hemoglobins from Clostridium perfringens an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047389d

    authors： Farrés J,Rechsteiner MP,Herold S,Frey AD,Kallio PT

    更新日期：2005-03-15 00:00:00

  • Spectroscopic evidence that osmolytes used in crystallization buffers inhibit a conformation change in a membrane protein.

    abstract：:BtuB is a bacterial outer-membrane protein that transports vitamin B(12). Spectroscopic studies using site-directed spin labeling (SDSL) indicate that the N-terminus of BtuB undergoes a dramatic structural change from a docked (folded) to an undocked (unfolded) configuration upon substrate binding. However, this drama...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035439t

    authors： Fanucci GE,Lee JY,Cafiso DS

    更新日期：2003-11-18 00:00:00

  • Modification of aldose reductase by S-nitrosoglutathione.

    abstract：:Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9714722

    authors： Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

    更新日期：1997-12-16 00:00:00

  • The Q-loop of DrrA is involved in producing the closed conformation of the nucleotide binding domains and in transduction of conformational changes between DrrA and DrrB.

    abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701699a

    authors： Rao DK,Kaur P

    更新日期：2008-03-04 00:00:00

  • Raman spectroscopy of synthetic antimicrobial frog peptides magainin 2a and PGLa.

    abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00470a031

    authors： Williams RW,Starman R,Taylor KM,Gable K,Beeler T,Zasloff M,Covell D

    更新日期：1990-05-08 00:00:00

  • Linkage and sequence analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopy.

    abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00559a031

    authors： Cohen RE,Ballou CE

    更新日期：1980-09-02 00:00:00

  • High sensitivity amino acid sequence determination. Application to proteins eluted from polyacrylamide gels.

    abstract：:An automatic solid-phase procedure is described for determining the amino-terminal amino acid sequence of very small quantities of proteins. The sample is covalently attached to an inert support so that mechanical and physical losses during sequencing are eliminated. High sensitivity is achieved by using an initial co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00661a030

    authors： Bridgen J

    更新日期：1976-08-10 00:00:00

  • Amyloid formation via supramolecular peptide assemblies.

    abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700247y

    authors： Moore RA,Hayes SF,Fischer ER,Priola SA

    更新日期：2007-06-19 00:00:00

  • A single-stranded DNA binding protein that specifically recognizes cis-acting sequences in the replication origin and transcriptional promoter region of Tetrahymena rDNA.

    abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00014a011

    authors： Hou Z,Umthun AR,Dobbs DL

    更新日期：1995-04-11 00:00:00

  • Electron-transferring enzymes in the plasma membrane of the Ehrlich ascites tumor cell.

    abstract：:The plasma membrane of the Ehrlich ascites tumor cell contains an NADH dehydrogenase. This activity was shown not to be due to contamination by other subcellular membranes. A variety of electron acceptors have been compared as to rate with the following result: ferricyanide greater than cytochrome c greater than cytoc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00575a021

    authors： Kilberg MS,Christensen HN

    更新日期：1979-04-17 00:00:00

  • Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

    abstract：:The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a021

    authors： Sims PJ,Wiedmer T

    更新日期：1984-07-03 00:00:00

  • Membrane topology of human ASBT (SLC10A2) determined by dual label epitope insertion scanning mutagenesis. New evidence for seven transmembrane domains.

    abstract：:The membrane topology of the human apical sodium-dependent bile acid transporter (hASBT) remains unresolved. Whereas N-glycosylation analysis favors a 7 transmembrane (TM) model, membrane insertion scanning supports a 9TM topology. In order to resolve this controversy, we used dual label epitope insertion to systemati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052202j

    authors： Banerjee A,Swaan PW

    更新日期：2006-01-24 00:00:00

  • Synthesis of dihydrothymidine and thymidine glycol 5'-triphosphates and their ability to serve as substrates for Escherichia coli DNA polymerase I.

    abstract：:5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00377a042

    authors： Ide H,Melamede RJ,Wallace SS

    更新日期：1987-02-10 00:00:00

  • Cooperativity of papain-substrate interaction energies in the S2 to S2' subsites.

    abstract：:Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00219a033

    authors： Berti PJ,Faerman CH,Storer AC

    更新日期：1991-02-05 00:00:00

  • Light-induced changes in the chemical bond structure of light-harvesting complex II probed by FTIR spectroscopy.

    abstract：:Light-harvesting complex II (LHC-II) regulates the light energy distribution between photosystem I and II in plants. This process is mediated by phosphorylation of the LHC-II protein, which depends on the oxidation state of photosynthetic electron carriers. In addition to this regulatory mechanism, it has recently bee...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034114+

    authors： Rogl H,Kühlbrandt W,Barth A

    更新日期：2003-09-02 00:00:00

  • Peptide secondary structure induced by a micellar phospholipidic interface: proton NMR conformational study of a lipopeptide.

    abstract：:The conformational change of the model peptide Ac-K-G-R-G-D-G-amide induced by a phospholipidic interface was investigated by proton nuclear magnetic resonance (1H NMR). In aqueous solution, the free peptide is highly flexible and disordered, even in the presence of deuterated dodecyl-phosphocholine (DPC-d38) micelles...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00124a018

    authors： Macquaire F,Baleux F,Giaccobi E,Huynh-Dinh T,Neumann JM,Sanson A

    更新日期：1992-03-10 00:00:00

  • Antisense L/D-oligodeoxynucleotide chimeras: nuclease stability, base-pairing properties, and activity at directing ribonuclease H.

    abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00191a015

    authors： Damha MJ,Giannaris PA,Marfey P

    更新日期：1994-06-28 00:00:00

  • Thermodynamic studies of the core histones: stability of the octamer subunits is not altered by removal of their terminal domains.

    abstract：:We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0110140

    authors： Karantza V,Freire E,Moudrianakis EN

    更新日期：2001-10-30 00:00:00

  • Determination of the rate-limiting steps for malic enzyme by the use of isotope effects and other kinetic studies.

    abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00623a002

    authors： Schimerlik MI,Grimshaw CE,Cleland WW

    更新日期：1977-02-22 00:00:00

  • N-H···N Hydrogen Bonds Involving Histidine Imidazole Nitrogen Atoms: A New Structural Role for Histidine Residues in Proteins.

    abstract：:The amino acid histidine can play a significant role in the structure and function of proteins. Its various functions include enzyme catalysis, metal binding activity, and involvement in cation-π, π-π, salt-bridge, and other types of noncovalent interactions. Although histidine's imidazole nitrogens (Nδ and Nε) are kn...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00253

    authors： Krishna Deepak RN,Sankararamakrishnan R

    更新日期：2016-07-12 00:00:00

  • Single-channel recordings from purified acetylcholine receptors reconstituted in bilayers formed at the tip of patch pipets.

    abstract：:The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00279a003

    authors： Suarez-Isla BA,Wan K,Lindstrom J,Montal M

    更新日期：1983-05-10 00:00:00

  • Characterization of an Arabidopsis calmodulin-like domain protein kinase purified from Escherichia coli using an affinity sandwich technique.

    abstract：:A full-length cDNA encoding a calcium-dependent protein kinase with a calmodulin-like domain from Arabidopsis thaliana (AK-1 for Arabidopsis kinase-1) has been expressed as a fusion protein (called AK-1-6H) in Escherichia coli and purified to near homogeneity with high specific activity (typically 2000 nmol min-1 mg-1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00174a008

    authors： Binder BM,Harper JF,Sussman MR

    更新日期：1994-03-01 00:00:00

  • Conformational drift and cryoinactivation of lactate dehydrogenase.

    abstract：:Solutions of porcine lactate dehydrogenase of micromolar concentration kept at 4 degrees C for several days lose the greater part of their enzymic activity but recover it when returned to room temperature. The rate of spoiling decreases and the rate of recovery increases with the concentration of the solutions. The de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00360a024

    authors： King L,Weber G

    更新日期：1986-06-17 00:00:00

  • NMR characterization of substrate binding in the phthalate dioxygenase system.

    abstract：:The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990431y

    authors： Tierney DL,Gassner GT,Luchinat C,Bertini I,Ballou DP,Penner-Hahn JE

    更新日期：1999-08-24 00:00:00

  • Effect of temperature on the intrinsic flexibility of DNA and its interaction with architectural proteins.

    abstract：:The helical structure of double-stranded DNA is destabilized by increasing temperature. Above a critical temperature (the melting temperature), the two strands in duplex DNA become fully separated. Below this temperature, the structural effects are localized. Using tethered particle motion in a temperature-controlled ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500344j

    authors： Driessen RP,Sitters G,Laurens N,Moolenaar GF,Wuite GJ,Goosen N,Dame RT

    更新日期：2014-10-21 00:00:00

  • pH-Dependent conformational stability of the ribotoxin alpha-sarcin and four active site charge substitution variants.

    abstract：:Alpha-sarcin is an exquisitely specific ribonuclease that binds and cleaves a single phosphodiester bond in the large rRNA of the eukaryotic ribosome, inactivating it. To better understand this remarkable activity, the contributions of the active site residues (His 50, Glu 96, and His 137) to the conformational stabil...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061273v

    authors： García-Mayoral MF,del Pozo AM,Campos-Olivas R,Gavilanes JG,Santoro J,Rico M,Laurents DV,Bruix M

    更新日期：2006-11-21 00:00:00

  • Molecular basis for membrane pore formation by Bax protein carboxyl terminus.

    abstract：:Bax protein plays a key role in mitochondrial membrane permeabilization and cytochrome c release upon apoptosis. Our recent data have indicated that the 20-residue C-terminal peptide of Bax (BaxC-KK; VTIFVAGVLTASLTIWKKMG), when expressed intracellularly, translocates to the mitochondria and exerts lethal effect on can...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301195f

    authors： Tatulian SA,Garg P,Nemec KN,Chen B,Khaled AR

    更新日期：2012-11-20 00:00:00

  • Direct identification of the agonist binding site in the human brain cholecystokininB receptor.

    abstract：:In investigating the agonist binding site of the human brain cholecystokininB receptor (CCKBR), we employed the direct protein chemical approach using a photoreactive tritiated analogue of sulfated cholecystokinin octapeptide, which contains the p-benzoylbenzoyl moiety at the N-terminus, followed by purification of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990269z

    authors： Anders J,Blüggel M,Meyer HE,Kühne R,ter Laak AM,Kojro E,Fahrenholz F

    更新日期：1999-05-11 00:00:00