Abstract：
:The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate interacts with the mononuclear metal of PDO and provide the first structural characterization of this interaction. With a molecular mass of 200 kDa, PDO is one of the largest proteins studied to date by paramagnetic NMR. Two paramagnetically broadened (19)F lines were observed for monofluorophthalates bound to CoPDO. This demonstrates that fluorophthalate binds to PDO with a handedness, i.e., with the fluorine label facing to the "right" or to the "left", relative to the hyperfine tensor of the Co(II). The relative affinities of the two orientations are slightly different, with a 2-fold and 5-fold excess of the preferred orientation for 4-fluorophthalate and 3-fluorophthalate, respectively. The longitudinal relaxation rate (T(1)) and transverse relaxation rate (T(2)) data give mutually consistent fluorine to cobalt distances. These results are consistent with approximate bilateral symmetry, with the Co to 3-fluorophthalate distances ( approximately 5.5 A) approximately 25% longer than the Co to 4-fluorophthalate distances ( approximately 4. 5 A). A detailed geometric model is derived from these data. This structural characterization of the mononuclear site provides a framework to develop hypotheses for the mechanism of oxygenation by the Fe(II)-containing aromatic dioxygenases.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tierney DL,Gassner GT,Luchinat C,Bertini I,Ballou DP,Penner-Hahn JEdoi
10.1021/bi990431ysubject
Has Abstractpub_date
1999-08-24 00:00:00pages
11051-61issue
34eissn
0006-2960issn
1520-4995pii
bi990431yjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The binding of ferric ions, chelated with nitrilotriacetate, to human serum transferrin (hTF) has been studied using ultrasensitive titration calorimetry. Studies were done in both the presence and the absence of the synergistic bicarbonate anion. It was found that the C-site of hTF is capable of weakly binding bicarb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00087a019
更新日期：1993-09-14 00:00:00
abstract：:The identity of the sites of insulin-stimulated serine phosphorylation in the human insulin receptor was examined by synthesizing peptides that together encompassed all the serine residues of the cytosolic portion of the beta-subunit and testing them as substrates for phosphorylation by a preparation of human insulin ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00029a025
更新日期：1995-07-25 00:00:00
abstract：:The production of proteins via recombinant DNA technology often requires the in vitro folding of inclusion bodies, which are protein aggregates. To create a more efficient redox buffer for the in vitro folding of disulfide containing proteins, aromatic thiols were investigated for their ability to increase the folding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034305c
更新日期：2003-10-14 00:00:00
abstract：:NADH oxidase activity has been detected in luminal membrane derived from the proximal tubules of rat kidney (brush border membranes). The cytochromes associated with these membranes [García, M.L., Benavides, J., Valdivieso, F., Mayor, F., & Giménez-Gallego, G. (1978) Biochem. Biophys. Res. Commun. 82, 738-744] were re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00562a019
更新日期：1980-10-14 00:00:00
abstract：:The pentraxin serum amyloid P component (SAP) is secreted by the liver and found in plasma at a concentration of approximately 30 mg/L. SAP is a 25 kDa homopentamer known to bind both protein and nonprotein ligands, all in a calcium-dependent manner. The function of SAP is unclear but likely involves the humoral innat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01027
更新日期：2017-02-14 00:00:00
abstract：:The crystal structures of the deoxy and carbonmonoxy forms of a distal pocket myoglobin mutant in which valine68(E11) is replaced by threonine have been solved to 2.1- and 2.2-A resolution, respectively. This substitution has been shown previously to cause large decreases in the rate of oxygen binding and to lower the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00211a016
更新日期：1993-12-07 00:00:00
abstract：:The triplet lifetime and absorption anisotropy decay of eosin-labeled band 3 was measured in resealed erythrocyte ghosts. Membrane potentials were generated by the addition of valinomycin in the presence of a K+ gradient. Neither negative nor positive membrane potentials had any detectable effect on the rotational dif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00134a016
更新日期：1992-05-19 00:00:00
abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a006
更新日期：1991-06-25 00:00:00
abstract：:In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00010a025
更新日期：1995-03-14 00:00:00
abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a002
更新日期：1992-07-21 00:00:00
abstract：:Each regulatory subunit of the cAMP-dependent protein kinase contains two in-tandem cAMP binding sites. Photolabeling of holoenzyme I with 8-azidoadenosine 3',5'-monophosphate (8-N3-cAMP) leads to the covalent modification of two residues, Trp-260 and Tyr-371. In order to correlate photolabeling of these two residues ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a035
更新日期：1987-06-16 00:00:00
abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0515669
更新日期：2006-02-14 00:00:00
abstract：:Because of the conflicting conclusions that have been reached regarding the location of the two putative membrane-spanning segments from cysteine 911 through isoleucine 929 and from isoleucine 946 through cysteine 964 in the alpha subunit of native ovine Na+/K(+)-transporting ATPase, the disposition of lysine 943 with...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00029a027
更新日期：1995-07-25 00:00:00
abstract：:ABCG2 is a half-ATP binding cassette (ABC) drug transporter that consists of a nucleotide binding domain (NBD) followed by a transmembrane domain. This half-ABC transporter is thought to form a homodimer in the plasma membrane where it transports anticancer drugs across the biological membranes in an ATP-dependent man...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900675v
更新日期：2009-09-29 00:00:00
abstract：:Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301241q
更新日期：2012-12-04 00:00:00
abstract：:Two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (RubisCO) enzymes were constructed using RubisCO small subunit genes (rbcS) from two eucaryotic marine organisms, Cylindrotheca sp. N1 and Olisthodiscus luteus, cloned downstream of the RubisCO large subunit gene (rbcL) of the cyanobacterium Synechococcus PCC ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00139a018
更新日期：1992-06-23 00:00:00
abstract：:The salivary antimicrobial peptide histatin-5 is able to aggregate and fuse negatively charged small unilamellar vesicles, and this fusogenic activity is selectively induced by the presence of zinc ions. Circular dichroism spectroscopy shows that histatin-5, in the presence of negatively charged vesicles and zinc ions...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990212c
更新日期：1999-07-27 00:00:00
abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047591g
更新日期：2005-05-10 00:00:00
abstract：:Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00415a030
更新日期：1988-07-26 00:00:00
abstract：:ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00770
更新日期：2020-12-01 00:00:00
abstract：:Sequential 1H NMR assignments of a metallothionein from Neurospora crassa have been accomplished by the combined use of COSY, 2QF-COSY, HOHAHA, and rotating-frame NOE experiments. All potentially observable resonances were assigned except for the epsilon-NH3 group of the C-terminal lysine. 1H NOEs, when observed in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00433a038
更新日期：1989-04-04 00:00:00
abstract：:4-Azido-2-nitrophenyl pyrophosphate (azido-PPi) labeled with 32P in the alpha position was prepared and used to photolabel beef heart mitochondrial F1. Azido-PPi was hydrolyzed by yeast inorganic pyrophosphatase, but not by mitochondrial F1-ATPase. Incubation of F1 with [alpha-32P]azido-PPi in the dark under condition...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00452a021
更新日期：1989-12-26 00:00:00
abstract：:Aerobic and facultative bacteria and archaea harboring mer loci exhibit resistance to the toxic effects of Hg(II) and organomercurials [RHg(I)]. In broad spectrum resistance, RHg(I) is converted to less toxic Hg(0) in the cytosol by the sequential action of organomercurial lyase (MerB: RHg(I) → RH + Hg(II)) and mercur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100802k
更新日期：2010-09-21 00:00:00
abstract：:The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00234
更新日期：2016-07-05 00:00:00
abstract：:The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963030g
更新日期：1997-08-19 00:00:00
abstract：:The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00231a022
更新日期：1991-04-30 00:00:00
abstract：:The molecular organization of sterols in liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at 37 degrees C is examined by utilizing the fluorescent analogue of cholesterol cholesta-5,7,9-trien-3 beta-ol (cholestatrienol). (1) Cholestatrienol is shown to be indistinguishable from native cholesterol i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00456a027
更新日期：1990-01-30 00:00:00
abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00272a026
更新日期：1983-02-01 00:00:00
abstract：:The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00411a004
更新日期：1988-05-31 00:00:00
abstract：:The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00040a012
更新日期：1995-10-10 00:00:00
