Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

abstract：:The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...

journal_title：Biochemistry

authors： Cosman M,Hingerty BE,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-11-21 00:00:00

abstract：:P450cam from Pseudomonas putida is the best characterized member of the vast family of cytochrome P450s, and it has long been believed to have a more rigid and closed active site relative to other P450s. Here we report X-ray structures of P450cam crystallized in the absence of substrate and at high and low [K(+)]. The...

journal_title：Biochemistry

authors： Lee YT,Wilson RF,Rupniewski I,Goodin DB

更新日期：2010-04-27 00:00:00

abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

journal_title：Biochemistry

authors： Sturtevant JM,Velicelebi G

更新日期：1981-05-26 00:00:00

abstract：:The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for rate enhancement (k(cat)/k(non) = 7.1 × 10(16)) and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(-1)]. Although a stabilized vinyl carbanion/carbene intermediate is located on the reactio...

journal_title：Biochemistry

authors： Iiams V,Desai BJ,Fedorov AA,Fedorov EV,Almo SC,Gerlt JA

更新日期：2011-10-04 00:00:00

abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...

journal_title：Biochemistry

authors： Zeidel ML,Nielsen S,Smith BL,Ambudkar SV,Maunsbach AB,Agre P

更新日期：1994-02-15 00:00:00

abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...

journal_title：Biochemistry

authors： Cao H,Pietrak BL,Grubmeyer C

更新日期：2002-03-12 00:00:00

abstract：:Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...

journal_title：Biochemistry

authors： van Meer G,Davoust J,Simons K

更新日期：1985-07-02 00:00:00

abstract：:Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the d...

journal_title：Biochemistry

authors： Clausen H,McKibbin JM,Hakomori S

更新日期：1985-10-22 00:00:00

abstract：:A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...

journal_title：Biochemistry

authors： Grant PG,Colman RW

更新日期：1984-04-10 00:00:00

abstract：:The kinetics of Zn(2+) binding by two point-mutated forms of the HIV-1 NCp7 C-terminal zinc finger, each containing tridentate binding motif HCC [Ser49(35-50)NCp7] or CCC [Ala44(35-50)NCp7], has been studied by stopped-flow spectrofluorimetry. Both the formation and dissociation rate constants of the complexes between...

journal_title：Biochemistry

authors： Bombarda E,Roques BP,Mély Y,Grell E

更新日期：2005-05-17 00:00:00

abstract：:Fluorescein 5'-isothiocyanate (FITC) was used to modify the lysine residues of bovine transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells. The incorporation of FITC showed a stoichiometry of approximately 1 mol of FITC/mol of transducin. The labeling was specific for the T al...

journal_title：Biochemistry

authors： Hingorani VN,Ho Y

更新日期：1987-03-24 00:00:00

abstract：:Human liver alcohol dehydrogenase [alcohol:NAD+ oxidoreductase, EC 1.1.1.1 (ADH)] catalyzes the stereospecific oxidation of different 3 beta-hydroxy-5 beta-steroids with ranges of Km from 46 to 320 microM and values of kcat from 7.0 to 72 min-1, pH 8.5. Only the class I isozymes containing gamma-subunits, gamma 1 gamm...

journal_title：Biochemistry

authors： McEvily AJ,Holmquist B,Auld DS,Vallee BL

更新日期：1988-06-14 00:00:00

abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...

journal_title：Biochemistry

authors： Bhatnagar RS,Schall OF,Jackson-Machelski E,Sikorski JA,Devadas B,Gokel GW,Gordon JI

更新日期：1997-06-03 00:00:00

abstract：:This paper describes the effect of N-capping substitutions on the structure and stability of histidine-containing protein (HPr). We have used NMR spectroscopy and conformational stability studies to quantify changes in local and global free energy due to mutagenesis at Ser46, the N-cap for helix B in HPr. Previous NMR...

journal_title：Biochemistry

authors： Thapar R,Nicholson EM,Rajagopal P,Waygood EB,Scholtz JM,Klevit RE

更新日期：1996-09-03 00:00:00

abstract：:2-Azidoadenosine 5'-diphosphate (2-azido-ADP) labeled with 32P in the alpha-position was prepared and used to photolabel the nucleotide binding sites of beef heart mitochondrial F1-ATPase. The native F1 prepared by the procedure of Knowles and Penefsky [Knowles, A. F., & Penefsky, H. S. (1972) J. Biol. Chem. 247, 6617...

journal_title：Biochemistry

authors： Boulay F,Dalbon P,Vignais PV

更新日期：1985-12-03 00:00:00

abstract：:An empirical preduction algorithm which uses information on the shrs on either side) interactions in 20 proteins to assign every residue in a protein to one of five conformational states is described. The conformational states are defined in terms of the backbone dihedral angles of the residue so that the prediction a...

journal_title：Biochemistry

authors： Maxfield FR,Scheraga HA

更新日期：1976-11-16 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...

journal_title：Biochemistry

authors： Wirsching P,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:The equilibrium binding of hemoglobin to isolated band 3 protein exhibited positive cooperativity [Hill coefficient = 1.65 +/- 0.1; total number of binding sites at pH 6.6 in 5 mM sodium phosphate buffer = 32 500 +/- 940 pmol/mg; Ka = (3.0 +/- 0.5) X 10(5) M-1]. The binding was reversible and ionic in nature as the bo...

journal_title：Biochemistry

authors： Premachandra BR

更新日期：1986-06-03 00:00:00

abstract：:We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...

journal_title：Biochemistry

authors： Ishiguro R,Matsumoto M,Takahashi S

更新日期：1996-04-16 00:00:00

abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...

journal_title：Biochemistry

authors： Morita I,Smith WL,DeWitt DL,Schindler M

更新日期：1995-05-30 00:00:00

abstract：:An improved procedure for the preparation of cobalt-cytochrome c has been developed. Various factors influencing the cobalt insertion process are discussed. The optical spectra of cobalt-cytochrome c suggest a six-coordinated species. The spectral shifts occurring with oxidation-reduction are compared with those obser...

journal_title：Biochemistry

authors： Dickinson LC,Chien JC

更新日期：1975-08-12 00:00:00

abstract：:Inwardly rectifying potassium channels are important in cellular repolarization of many excitable tissues. Amino acid sequence alignment of different mammalian inward rectifier K(+) channels revealed two absolutely conserved cysteine residues in the putative extracellular face, suggesting a possible disulfide bond. Re...

journal_title：Biochemistry

authors： Cho HC,Tsushima RG,Nguyen TT,Guy HR,Backx PH

更新日期：2000-04-25 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

journal_title：Biochemistry

authors： Eckl JM,Drazic A,Rutz DA,Richter K

更新日期：2014-04-22 00:00:00

abstract：:The X-ray crystal structure of a salicylhydroxamic acid (SHA) inhibitory complex with human myeloperoxidase (MPO) has been determined at 2.3 A resolution. The aromatic ring of the inhibitor binds to a hydrophobic region at the entrance to the distal heme pocket between heme pyrrole ring D and the side chain of Arg 239...

journal_title：Biochemistry

authors： Davey CA,Fenna RE

更新日期：1996-08-20 00:00:00

abstract：:When Pseudomonas putida ATCC 39167 and plant-deleterious Pseudomonas fluorescens A225 were grown in an iron-deficient culture medium, they each produced two different novel yellow-green fluorescent pseudobactins: P39167-I, II and PA225-I, II. Pseudobactin P39167-I has a molecular formula of C46H65O23N13 and is monoani...

journal_title：Biochemistry

authors： Khalil-Rizvi S,Toth SI,van der Helm D,Vidavsky I,Gross ML

更新日期：1997-04-08 00:00:00

abstract：:Escherichia coli alkaline phosphatase (AP) is the prototypical two metal ion catalyst with two divalent zinc ions bound approximately 4 A apart in the active site. Studies spanning half a century have elucidated many structural and mechanistic features of this enzyme, rendering it an attractive model for investigating...

journal_title：Biochemistry

authors： O'Brien PJ,Herschlag D

更新日期：2002-03-05 00:00:00

abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

journal_title：Biochemistry

authors： McCutchen SL,Colon W,Kelly JW

更新日期：1993-11-16 00:00:00

abstract：:Nanosecond to millisecond conformational fluctuations of Zn-substituted cytochrome c (ZnCytc) have been studied by the time-resolved transient hole-burning method. The investigation of low-temperature dynamics has been made on the ZnCytc solution sample in a water-glycerol mixture. The conformational fluctuations in t...

journal_title：Biochemistry

authors： Shibata Y,Takahashi H,Kaneko R,Kurita A,Kushida T

更新日期：1999-02-09 00:00:00

abstract：:Two short amino acid motifs, WXGDXNXR and PXWCDRXL, define a large family of inositol polyphosphate 5-phosphatases. We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphata...

journal_title：Biochemistry

authors： Jefferson AB,Majerus PW

更新日期：1996-06-18 00:00:00