Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

abstract：:OleD is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in Stenotrophomonas maltophilia. The gene coding for OleD clusters with three other genes, oleABC, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. In this stu...

journal_title：Biochemistry

authors： Bonnett SA,Papireddy K,Higgins S,del Cardayre S,Reynolds KA

更新日期：2011-11-08 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:We have obtained a quantitative description of villin-nucleated actin polymerization in physiological salt by determining the concentrations of free villin (V), villin-actin monomer (VA), villin-actin dimer (VA2), and villin-actin oligomer (VAn). Over a range of actin-villin ratios from 0.1 to 20 we determined the con...

journal_title：Biochemistry

authors： Weber A,Northrop J,Bishop MF,Ferrone FA,Mooseker MS

更新日期：1987-05-05 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:Apolipoprotein B (apoB) is a nonexchangeable apolipoprotein that dictates the synthesis of chylomicrons and very low density lipoproteins. ApoB is the major protein in low density lipoprotein, also known as the "bad cholesterol" that is directly implicated in atherosclerosis. It has been suggested that the N-terminal ...

journal_title：Biochemistry

authors： Jiang ZG,Gantz D,Bullitt E,McKnight CJ

更新日期：2006-10-03 00:00:00

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:We determined the effect of vertebrate albumins on the stability of several physiologically relevant prostaglandins. All naturally occurring prostaglandins with beta-hydroxy ketone group decomposed by first-order kinetics, dependent on the albumin concentration in 0.1 M, pH 7.4, buffer at 37 degrees C. Even subphysiol...

journal_title：Biochemistry

authors： Fitzpatrick FA,Waynalda MA

更新日期：1981-10-13 00:00:00

abstract：:We previously isolated a unique anticoagulant protein named IX/X-bp (factor IX/factor X-binding protein) from the venom of the habu snake Trimeresurus flavoviridis. We recently determined its primary structure and found that this protein had a structure homologous to the carbohydrate-recognition domains of C-type lect...

journal_title：Biochemistry

authors： Sekiya F,Atoda H,Morita T

更新日期：1993-07-13 00:00:00

abstract：:The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...

journal_title：Biochemistry

authors： Stannard C,Soskic V,Godovac-Zimmermann J

更新日期：2003-12-02 00:00:00

abstract：:DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...

journal_title：Biochemistry

authors： Toulmin A,Baltierra-Jasso LE,Morten MJ,Sabir T,McGlynn P,Schröder GF,Smith BO,Magennis SW

更新日期：2017-09-19 00:00:00

abstract：:Snake venom contains a number of the hemostatically active C-type lectin-like proteins, which affect the interaction between von Willebrand factor (vWF) and the platelet glycoprotein (GP) Ib or platelet receptor to inhibit/induce platelet activation. Flavocetin-A (FL-A) is a high-molecular mass C-type lectin-like prot...

journal_title：Biochemistry

authors： Fukuda K,Mizuno H,Atoda H,Morita T

更新日期：2000-02-29 00:00:00

abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...

journal_title：Biochemistry

authors： Kealey JT,Santi DV

更新日期：1991-10-08 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:One of the putative proton-transfer pathways leading from solution toward the binuclear center in many cytochrome c oxidases is the D-pathway, so-called because it starts with a highly conserved aspartate [D(I-132)] residue. Another highly conserved amino acid residue in this pathway, glutamate(I-286), has been indica...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Mills DA,Ferguson-Miller S,Brzezinski P

更新日期：2000-12-26 00:00:00

abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...

journal_title：Biochemistry

authors： Zägel P,Dell'Orco D,Koch KW

更新日期：2013-07-30 00:00:00

abstract：:Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...

journal_title：Biochemistry

authors： Eldridge CA,Elson EL,Webb WW

更新日期：1980-05-13 00:00:00

abstract：:The ATF (activating transcription factor) binding site within the E2A promoter region of adenovirus is shown to exist in a novel conformation in vitro via nuclear magnetic resonance methods. This novel conformation may be important to the protein DNA recognition process. This conformation has characteristics of both A...

journal_title：Biochemistry

authors： Borden KL

更新日期：1993-07-06 00:00:00

abstract：:The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...

journal_title：Biochemistry

authors： Jiang W,Yun D,Saleh L,Bollinger JM Jr,Krebs C

更新日期：2008-12-30 00:00:00

abstract：:The physicochemical and catalytic properties of thioredoxin-T' are described. This complemented protein structure consists of a 1:1 complex between the inactive fragments thioredoxin-T-(1--73) and thioredoxin T-(74--108). These are generated by selective trypsin cleavage at Arg-73 in lysine-modified and denatured Esch...

journal_title：Biochemistry

authors： Slabý I,Holmgren A

更新日期：1979-12-11 00:00:00

abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...

journal_title：Biochemistry

authors： Jacobs DL,Watt GD,Frankel RB,Papaefthymiou GC

更新日期：1989-02-21 00:00:00

abstract：:ncd is a kinesin-related motor protein from Drosophila that moves in the opposite direction along microtubules to kinesin. To learn more about the ncd mechanism, ncd motor domain (R335-K700) was expressed in Escherichia coli and its enzymatic characteristics were studied. The ncd motor domain was purified from the cel...

journal_title：Biochemistry

authors： Shimizu T,Sablin E,Vale RD,Fletterick R,Pechatnikova E,Taylor EW

更新日期：1995-10-10 00:00:00

abstract：:Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...

journal_title：Biochemistry

authors： Blackburn JM,Sutherland JD,Baldwin JE

更新日期：1995-06-06 00:00:00

abstract：:Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...

journal_title：Biochemistry

authors： Kudlicki W,Chirgwin J,Kramer G,Hardesty B

更新日期：1995-11-07 00:00:00

abstract：:The use of cefoxitin, a poor substrate of the RTEM beta-lactamase, has allowed the kinetic and spectroscopic characterization of a covalent acyl-enzyme intermediate in the enzyme-catalyzed reaction. The rate of reappearance of catalytic activity in an enzyme sample diluted from an incubation with cefoxitin is nearly i...

journal_title：Biochemistry

authors： Fisher J,Belasco JG,Khosla S,Knowles JR

更新日期：1980-06-24 00:00:00

abstract：:The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...

journal_title：Biochemistry

authors： Dabrowska R,Sherry JM,Aromatorio DK,Hartshorne DJ

更新日期：1978-01-24 00:00:00

abstract：:Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...

journal_title：Biochemistry

authors： Colon W,Kelly JW

更新日期：1992-09-15 00:00:00

abstract：:The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...

journal_title：Biochemistry

authors： Yeagle PL,Martin RB,Pottenger L,Langdon RG

更新日期：1978-07-11 00:00:00

abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...

journal_title：Biochemistry

authors： Holzenburg A,Engel A,Kessler R,Manz HJ,Lustig A,Aebi U

更新日期：1989-05-16 00:00:00

abstract：:We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...

journal_title：Biochemistry

authors： Zaheer A,Lim R

更新日期：1996-05-21 00:00:00

abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...

journal_title：Biochemistry

authors： Ziehler WA,Day JJ,Fierke CA,Engelke DR

更新日期：2000-08-15 00:00:00