Abstract：
:A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. The protein has a molecular weight of 140 000 as determined by gel filtration. On NaDodSO4-containing polyacrylamide gels the major band is at 61 000 daltons, suggesting that the enzyme may exist as a dimer in solution under nondenaturing conditions. The enzyme requires Mg2+ or Mn2+ for activity. The calcium binding protein calmodulin does not stimulate hydrolysis of cAMP by this enzyme. The purified enzyme hydrolyzes both cAMP and cGMP with normal Michaelis-Menten kinetics with Km values of 0.18 microM and 0.02 microM, respectively. The hydrolysis of cGMP, however, is only one-tenth as rapid as the hydrolysis of cAMP. Cyclic GMP does not stimulate cAMP hydrolysis but instead is a potent competitive inhibitor of cAMP hydrolysis. The enzyme is also competitively inhibited by the phosphodiesterase inhibitors papaverine, 3-isobutyl-l-methylxanthine, and dipyridamole. The enzyme did not cross-react with an antibody raised to a cAMP phosphodiesterase isolated from dog kidney, indicating that the enzymes are not immunologically related. The inhibition of cAMP hydrolysis by cGMP suggests a possible regulatory link between these two cyclic nucleotides. One of the roles of cGMP in platelets may be to potentiate increases in intracellular cAMP by inhibiting the hydrolysis of cAMP by this enzyme.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Grant PG,Colman RWdoi
10.1021/bi00303a034subject
Has Abstractpub_date
1984-04-10 00:00:00pages
1801-7issue
8eissn
0006-2960issn
1520-4995journal_volume
23pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0353272
更新日期：2003-12-09 00:00:00
abstract：:Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin (TTX) have been synthesized. N-Methylanthraniloylglycine hydrazide, anthraniloyl hydrazide, and 2-azidoanthraniloylglycine hydrazide were coupled to the carbonyl at C6 of oxidized tetrodotoxin to form stable fluorescent hydrazones. The C6 ketone ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00517a025
更新日期：1981-07-07 00:00:00
abstract：:Human DNA polymerase kappa (pol kappa) has a sequence significantly homologous with that of Escherichia coli DNA polymerase IV (pol IV). We used a truncated form of human pol kappa (pol kappaDeltaC) and full-length pol IV to explore the miscoding properties of these enzymes. Oligodeoxynucleotides, modified site-specif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010702g
更新日期：2001-12-18 00:00:00
abstract：:The microtubule-associated protein tau, in a hyperphosphorylated form, aggregates into insoluble paired-helical filaments (PHFs) in Alzheimer's disease (AD) and other tauopathies. In AD, there is approximately 8 mol of phosphate per mole of tau distributed among approximately 30 PHF phosphorylation sites as compared t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900602h
更新日期：2009-06-30 00:00:00
abstract：:We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00170a011
更新日期：1994-02-01 00:00:00
abstract：:Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide transporters, e.g., from kidney and small intestine of rabbit, man, and rat. Hydropathy profiling in combination with the "pos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963068t
更新日期：1997-06-03 00:00:00
abstract：:Chlorophyll a fluorescence emission is widely used as a noninvasive measure of a number of parameters related to photosynthetic efficiency in oxygenic photosynthetic organisms. The most important component for the estimation of photochemistry is the relative increase in fluorescence yield between dark-adapted samples ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9806854
更新日期：1998-08-04 00:00:00
abstract：:The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b01084
更新日期：2020-04-07 00:00:00
abstract：:The present study was carried out to determine the energetics of Clostridium symbiosum pyruvate phosphate dikinase (PPDK) catalyzed interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (Pi), and pyruvate (pyr) with adenosine 5'-monophosphate (AMP), inorganic pyrophosphate, and phosphoenolpyruvate (PEP) [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00171a007
更新日期：1994-02-08 00:00:00
abstract：:Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00246a013
更新日期：1991-08-13 00:00:00
abstract：:Tau is a neuronal microtubule-associated protein that plays a central role in many cellular processes, both physiological and pathological, such as axons stabilization and Alzheimer's disease. Despite extensive studies, very little is known about the detailed molecular basis of tau binding to microtubules. We used the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0493160
更新日期：2004-08-17 00:00:00
abstract：:The clade B/intracellular serpins protect cells from peptidase-mediated injury by forming covalent complexes with their targets. SERPINB12 is expressed in most tissues, especially at cellular interfaces with the external environment. This wide tissue distribution pattern is similar to that of granzyme A (GZMA). Becaus...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01042
更新日期：2015-11-17 00:00:00
abstract：:Amyloid formation by immunoglobulin light chain (LC) proteins is associated with amyloid light chain (AL) amyloidosis. Destabilization of the native state of the variable domain of the LC (VL) is known to be one of the critical factors in promoting the formation of amyloid fibrils. However, determining the key residue...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5007892
更新日期：2014-08-12 00:00:00
abstract：:The aureolic acid antitumor antibiotic mithramycin (MTM) inhibits both cancer growth and bone resorption by cross-linking GC-rich DNA, thus blocking binding of Sp-family transcription factors to gene regulatory elements. Transcription of c-src, a gene implicated in many human cancers and required for osteoclast-depend...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034091z
更新日期：2003-07-15 00:00:00
abstract：:The objective of this work is to determine the molecular mechanism and regulation of short-chain acyl-CoA dehydrogenase (SCAD) from Megasphaera elsdenii. To achieve this, the gene coding for SCAD from M. elsdenii was cloned and sequenced. Site-directed mutagenesis was then used to identify an amino acid residue that i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a026
更新日期：1993-10-12 00:00:00
abstract：:High-precision studies of oxygen binding in hemoglobin (HbA0) solutions at near-physiological concentrations (2-12 mM heme; pHs 7.0-9.1; various buffers) have led to an unanticipated result: an unmeasurably low contribution from the triply ligated species. We have obtained this result from new differential oxygen-bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00387a038
更新日期：1987-06-30 00:00:00
abstract：:Tissue factor pathway inhibitor (TFPI) from different cell lines shows up to 15-fold differences in the ratio of anticoagulant to chromogenic activity. The anticoagulant activity was dependent on the purification procedure used and it was possible to isolate two fractions of recombinant TFPI. Only one of these fractio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00107a002
更新日期：1991-10-29 00:00:00
abstract：:A photoaffinity probe for corticotropin (ACTH) receptors was prepared by selective modification of the single tryptophan residue in ACTH. A new photoreactive agent, 2,4-dinitro-5-azidophenylsulfenyl chloride, was synthesized and used for introducing the photoreactive group into ACTH. 2,4-Dinitro-5-azidophenylsulfenyl-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a027
更新日期：1980-07-08 00:00:00
abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7018578
更新日期：2008-02-05 00:00:00
abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00412a039
更新日期：1988-06-14 00:00:00
abstract：:STRA6 is a multitransmembrane domain protein not homologous to any other proteins with known function. It functions as the high-affinity receptor for plasma retinol binding protein (RBP) and mediates cellular uptake of vitamin A from the vitamin A-RBP complex. Consistent with the diverse roles of vitamin A and the wid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8002082
更新日期：2008-05-13 00:00:00
abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981679a
更新日期：1999-03-30 00:00:00
abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990185q
更新日期：1999-05-11 00:00:00
abstract：:Halorhodopsin from Natronomonas pharaonis (NpHR) acts an inward-directed, light-driven chloride pump and forms a homotrimer. To evaluate effect of trimeric assembly, that is, intermolecular interaction, on the control or modulation of light-driven chloride pumping activity of individual HRs, it is important to underst...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901380c
更新日期：2009-12-29 00:00:00
abstract：:The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00001a026
更新日期：1995-01-10 00:00:00
abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800770g
更新日期：2008-08-05 00:00:00
abstract：:The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00234a032
更新日期：1991-05-21 00:00:00
abstract：:Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00094a001
更新日期：1993-11-02 00:00:00
abstract：:The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0341096
更新日期：2003-05-20 00:00:00
abstract：:The coat protein of bacteriophage Pf3 forms discrete and stable ion channels of uniform size in planar bilayers of asolectin. Its primary sequence suggests a channel formed by a bundle of transmembrane helices. Since the apparent transmembrane region only consists of strongly hydrophobic residues, it represents a new ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00167a037
更新日期：1994-01-11 00:00:00
