Abstract：
:STRA6 is a multitransmembrane domain protein not homologous to any other proteins with known function. It functions as the high-affinity receptor for plasma retinol binding protein (RBP) and mediates cellular uptake of vitamin A from the vitamin A-RBP complex. Consistent with the diverse roles of vitamin A and the wide tissue expression pattern of STRA6, mutations in STRA6 are associated with severe pathological phenotypes in humans. The structural basis for STRA6's biochemical function is unknown. Although computer programs predict 11 transmembrane domains for STRA6, its topology has never been studied experimentally. Elucidating the transmembrane topology of STRA6 is critical for understanding its structure and function. By inserting an epitope tag into all possible extracellular and intracellular domains of STRA6, we systematically analyzed the accessibility of each tag on the surface of live cells, the accessibility of each tag in permeabilized cells, and the effect of each tag on RBP binding and STRA6-mediated vitamin A uptake from the vitamin A-RBP complex. In addition, we used a new lysine accessibility technique combining cell-surface biotinylation and tandem-affinity purification to study a region of the protein not revealed by the epitope tagging method. These studies not only revealed STRA6's extracellular, transmembrane, and intracellular domains but also implicated extracellular regions of STRA6 in RBP binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kawaguchi R,Yu J,Wiita P,Ter-Stepanian M,Sun Hdoi
10.1021/bi8002082subject
Has Abstractpub_date
2008-05-13 00:00:00pages
5387-95issue
19eissn
0006-2960issn
1520-4995journal_volume
47pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Ionizing radiation, free radicals, and reactive oxygen species produce hundreds of different DNA lesions. Clustered lesions are typical for ionizing radiation. They compromise the efficiency of the base excision repair (BER) pathway, and as a consequence, they are much more toxic and mutagenic than isolated lesions. D...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00396
更新日期：2016-07-19 00:00:00
abstract：:We have used thrombin to cleave apolipoprotein C-III-1 into two fragments constituting residues 1-40 (apoLP-C-III-A) and 41-79 (apoLP-C-III-B). The lipid binding properties of these fragments with dimyristoyl- and 1-palmitoyl-2-oleoylphosphatidylcholines have been determined using circular dichroic and intrinsic trypt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00644a004
更新日期：1977-12-13 00:00:00
abstract：:The conserved glycines in the glycine-rich loop (Leu-Gly50-Thr-Gly52-Ser-Phe-Gly55-Arg-Val) of the catalytic (C) subunit of cAMP-dependent protein kinase were each mutated to Ser (G50S, G52S, and G55S). The effects of these mutations were assessed here using both steady-state and pre-steady-state kinetic methods. Whil...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972987w
更新日期：1998-05-26 00:00:00
abstract：:Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052387u
更新日期：2006-04-11 00:00:00
abstract：:Phosphoprotein particles were isolated in their native state from the physiological fluid of the estuarine clam Rangia cuneata , and the characteristics of the mineral ion-protein complex which constitutes the native particle were investigated by using mineral ion binding and mineral ion exchange techniques. The parti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00302a017
更新日期：1984-03-27 00:00:00
abstract：:Staphylococcal nuclease H124L is a recombinant protein produced in Escherichia coli whose sequence is identical with that of the nuclease produced by the V8 variant of Staphylococcus aureus. The enzyme-metal ion activator-nucleotide inhibitor ternary complex, nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+, was inves...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00453a011
更新日期：1990-01-09 00:00:00
abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061134d
更新日期：2006-12-05 00:00:00
abstract：:β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500074w
更新日期：2014-03-18 00:00:00
abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00521a014
更新日期：1981-09-01 00:00:00
abstract：:PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052225y
更新日期：2006-03-21 00:00:00
abstract：:This report presents evidence for interdomain steps of the ligand-coupled signal transduction mechanism of the oxygen receptor from Rhizobium meliloti, RmFixL. Photolysis of the CO adducts of heme domain (RmFixLN) and heme kinase (RmFixL*) proteins allowed tracking of second-order heme CO recombination reactions by tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011237q
更新日期：2001-10-30 00:00:00
abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036229j
更新日期：2004-05-18 00:00:00
abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900811p
更新日期：2009-08-25 00:00:00
abstract：:De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102033a
更新日期：2011-05-31 00:00:00
abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00348a023
更新日期：1985-12-31 00:00:00
abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061722r
更新日期：2007-03-20 00:00:00
abstract：:Alpha-synuclein (alphaS) is a lipid-binding synaptic protein of unknown function that is found in an aggregated amyloid fibril form in the intraneuronal Lewy body deposits that are a defining characteristic of Parkinson's disease (PD). Although intrinsically unstructured when free in solution, alphaS adopts a highly h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036135+
更新日期：2004-04-27 00:00:00
abstract：:PKC phosphorylation regulates PITX2 DNA binding and transcriptional activity. Mutation of individual PKC sites demonstrates the functional regulation of PITX2 through phosphorylation. Immunoprecipitation of PITX2 and a PITX2 PKC mutant protein reveal specific in vivo phosphorylation by PKC in transfected cells. The tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048362x
更新日期：2005-03-15 00:00:00
abstract：:Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048548w
更新日期：2005-01-25 00:00:00
abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00487a005
更新日期：1990-09-04 00:00:00
abstract：:Prion diseases, also known as the transmissible spongiform encephalopathies (TSEs), are a group of fatal neurodegenerative disorders that affect humans and animals. These diseases are intimately associated with conformational conversion of the cellular prion protein, PrP(C), into an oligomeric beta-sheet-rich form, Pr...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi900108v
更新日期：2009-03-31 00:00:00
abstract：:The first epidermal growth factor-like domain (EGF-1) from blood coagulation factor VII (FVII) contains two unusual O-linked glycosylation sites at Ser-52 and Ser-60. We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990234z
更新日期：1999-06-01 00:00:00
abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052062d
更新日期：2006-04-25 00:00:00
abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9625509
更新日期：1997-04-29 00:00:00
abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992286u
更新日期：2000-03-21 00:00:00
abstract：:In this work we report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring its target site in 23S rRNA. The probe is complementary to 23S rRNA nucleotides 2497-2505, a single-stranded sequence that has been shown to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00236a014
更新日期：1991-06-04 00:00:00
abstract：:We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00435a011
更新日期：1989-05-02 00:00:00
abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a022
更新日期：1992-05-26 00:00:00
abstract：:The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00140a010
更新日期：1992-06-30 00:00:00
abstract：:A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00057a027
更新日期：1993-02-16 00:00:00
