Solution structures of spinach acyl carrier protein with decanoate and stearate.

abstract：:Phospholipase Cβ (PLCβ) enzymes hydrolyze phosphatidylinositol 4,5-bisphosphate to produce second messengers that regulate intracellular Ca2+, cell proliferation, and survival. Their activity is dependent upon interfacial activation that occurs upon localization to cell membranes. However, the molecular basis for how ...

journal_title：Biochemistry

authors： Hudson BN,Hyun SH,Thompson DH,Lyon AM

更新日期：2017-10-17 00:00:00

abstract：:We have used a fluorescent analogue of ATP, mantATP [2'(3')-O-(N-methylanthraniloyl)-adenosine 5'-triphosphate; Hiratsuka T. (1983) Biochim. Biophys. Acta 742, 496-508], and made a detailed kinetic study of the interaction of mantATP and mantADP with S1 and acto-S1. We have shown that these analogues behave like ATP a...

journal_title：Biochemistry

authors： Woodward SK,Eccleston JF,Geeves MA

更新日期：1991-01-15 00:00:00

abstract：:The "stalk" of the prokaryotic 50S ribosomal subunit is comprised of four copies of the protein L7/L12. In Escherichia coli, L7/L12 is a dimeric protein at micromolar concentrations, which is able to undergo rapid subunit exchange. A recent structural study indicated a tetrameric arrangement of the L12 proteins isolat...

journal_title：Biochemistry

authors： Moens PD,Wahl MC,Jameson DM

更新日期：2005-03-08 00:00:00

abstract：:Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...

journal_title：Biochemistry

authors： Bazzi MD,Nelsestuen GL

更新日期：1988-10-04 00:00:00

abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

journal_title：Biochemistry

authors： Rheinnecker M,Baker G,Eder J,Fersht AR

更新日期：1993-02-09 00:00:00

abstract：:RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...

journal_title：Biochemistry

authors： Malik SS,Rich E,Viswanathan R,Cairns BR,Fischer CJ

更新日期：2011-09-20 00:00:00

abstract：:His51 in horse liver alcohol dehydrogenase (ADHE) has been proposed to act as a proton donor/acceptor in the NAD+/NADH-dependent oxidation/reduction of alcohol/aldehyde. The residue corresponding to His51 of ADHE is Val51 (Val45 in the protein sequence) in benzyl alcohol dehydrogenase (BADH) encoded by TOL plasmid pWW...

journal_title：Biochemistry

authors： Inoue J,Tomioka N,Itai A,Harayama S

更新日期：1998-03-10 00:00:00

abstract：:The proton-translocating NADH-quinone oxidoreductase (NDH-1) of Paracoccus denitrificans is composed of 14 different subunits (Nqo1-Nqo14). Of these, seven subunits (Nqo7, Nqo8, and Nqo10-14) which are equivalent to the mitochondrial DNA-encoded subunits of complex I constitute the membrane segment of the enzyme compl...

journal_title：Biochemistry

authors： Kao MC,Di Bernardo S,Matsuno-Yagi A,Yagi T

更新日期：2003-04-22 00:00:00

abstract：:Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...

journal_title：Biochemistry

authors： Hutchinson JP,Eccleston JF

更新日期：2000-09-19 00:00:00

abstract：:A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of...

journal_title：Biochemistry

authors： Gitter-Amir A,Schneider AS,Rosenheck K

更新日期：1976-07-13 00:00:00

abstract：:The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a...

journal_title：Biochemistry

authors： Tararina MA,Janda KD,Allen KN

更新日期：2016-12-06 00:00:00

abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

journal_title：Biochemistry

authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

更新日期：2012-08-28 00:00:00

abstract：:Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...

journal_title：Biochemistry

authors： Marden MC,Hazard ES 3rd,Gibson QH

更新日期：1986-05-20 00:00:00

abstract：:We have carried out a comparative study of the effect of cholesterol on the thermotropic phase behavior of the distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine using high-sensitivity differential scanning calorimetry. For both molecular species of pho...

journal_title：Biochemistry

authors： McMullen TP,McElhaney RN

更新日期：1997-04-22 00:00:00

abstract：:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...

journal_title：Biochemistry

authors： Dimock K,Stoltzfus CM

更新日期：1977-02-08 00:00:00

abstract：:A method has been developed for the routine synthesis of 2'(3')-o-monoacyl ribonucleoside 5'-diphosphates for stepwise synthesis of oligoribonucleotides with Escherichia coli polynucleotide phosphorylase. The use of triethyl orthoisovalerate allows the facile preparation of 2'(3')-o-isovaleryl-UDP, -CDP, -ADP, -GDP, -...

journal_title：Biochemistry

authors： Walker GC,Uhlenbeck OC

更新日期：1975-02-25 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:An IscA homologue within the nif regulon of Azotobacter vinelandii, designated (Nif)IscA, was expressed in Escherichia coli and purified to homogeneity. Purified (Nif)IscA was found to be a homodimer of 11-kDa subunits that contained no metal centers or other prosthetic groups in its as-isolated form. Possible roles f...

journal_title：Biochemistry

authors： Krebs C,Agar JN,Smith AD,Frazzon J,Dean DR,Huynh BH,Johnson MK

更新日期：2001-11-20 00:00:00

abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...

journal_title：Biochemistry

authors： Khananshvili D,Baazov D,Weil-Maslansky E,Shaulov G,Mester B

更新日期：1996-12-10 00:00:00

abstract：:The catalytic (C) subunit of cAMP-dependent protein kinase interacts with two classes of inhibitors. The regulatory (R) subunits, types I and II, associate to form an inactive holoenzyme complex that is activated in response to cAMP. The C-subunit is also inhibited by small heat-stable protein kinase inhibitors (PKI's...

journal_title：Biochemistry

authors： Herberg FW,Taylor SS

更新日期：1993-12-21 00:00:00

abstract：:Alpha-synuclein (alpha-syn) is the major component of intracellular inclusions in several neurodegenerative diseases, and the conversion of soluble alpha-syn into filamentous aggregates may contribute to disease pathogenesis. Since mechanisms leading to the formation of alpha-syn inclusions are unclear, in vitro model...

journal_title：Biochemistry

authors： Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

更新日期：2003-07-22 00:00:00

abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...

journal_title：Biochemistry

authors： Bustin M,Simpson RT,Sperling R,Goldblatt D

更新日期：1977-11-29 00:00:00

abstract：:Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembl...

journal_title：Biochemistry

authors： García-Guzmán M,Sala F,Sala S,Campos-Caro A,Criado M

更新日期：1994-12-20 00:00:00

abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...

journal_title：Biochemistry

authors： Cirilli M,Zheng R,Scapin G,Blanchard JS

更新日期：1998-11-24 00:00:00

abstract：:Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle. Cosedimentation experiments revealed an approximately 3-fold decrease in the binding ...

journal_title：Biochemistry

authors： Miller CJ,Wong WW,Bobkova E,Rubenstein PA,Reisler E

更新日期：1996-12-24 00:00:00

abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

journal_title：Biochemistry

authors： Brodner OG,Wieland T

更新日期：1976-08-10 00:00:00

abstract：:Previous work has indicated that PEP mutase catalyzes the rearrangement of phosphoenolpyruvate to phosphonopyruvate by a dissociative mechanism. The crystal structure of the mutase with Mg(II) and sulfopyruvate (a phosphonopyruvate analogue) bound showed that the substrate is anchored to the active site by the Mg(II),...

journal_title：Biochemistry

authors： Liu S,Lu Z,Han Y,Jia Y,Howard A,Dunaway-Mariano D,Herzberg O

更新日期：2004-04-20 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00

abstract：:When partially purified Ehrlich ascites tumor RNA polymerase II was further purified on a column of phosphocellulose, stimulation of its catalysis of RNA synthesis by stimulatory factor S-II was greatly decreased. This decrease in sensitivity to the stimulatory factor was reversible: the enzyme eluted from phosphocell...

journal_title：Biochemistry

authors： Kuroiwa A,Mizuno D,Natori S

更新日期：1977-12-27 00:00:00

abstract：:Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...

journal_title：Biochemistry

authors： Williams AW,Dunlap RB,Berger SH

更新日期：1998-05-19 00:00:00