Abstract：
:A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of the major differences between cell and hemoglobin solution spectra were quantitatively accounted for in terms of differential scatter and absorption flattening, with the latter effect dominating the picture. The relatively low sensitivity of the red blood cell suspension circular dichroism spectrum to hemoglobin conformation is due to the order of magnitude flattening of circular dichroism intensity. The importance of accounting for instrumental light detection geometry and the intense small angle scattering (less than 8 degrees) for a range of particle sizes (0.1-5 mum) is made clear.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Gitter-Amir A,Schneider AS,Rosenheck Kdoi
10.1021/bi00659a030subject
Has Abstractpub_date
1976-07-13 00:00:00pages
3138-45issue
14eissn
0006-2960issn
1520-4995journal_volume
15pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The proton transport membrane protein bacteriorhodopsin has been biosynthetically labeled with [methyl-13C]methionine and studied by high-resolution 13C NMR after solubilization in the detergent Triton X-100. The nine methionine residues of bacteriorhodopsin give rise to four well-resolved 13C resonances, two of which...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00230a012
更新日期：1991-04-23 00:00:00
abstract：:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00349a020
更新日期：1986-01-14 00:00:00
abstract：:Ornithine decarboxylase (ODC) is a pyridoxal 5'-phosphate (PLP) dependent homodimeric enzyme. It is a recognized drug target against African sleeping sickness, caused by Trypanosoma brucei. One of the currently used drugs, alpha-difluoromethylornithine (DFMO), is a suicide inhibitor of ODC. The structure of the T. bru...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9915115
更新日期：1999-11-16 00:00:00
abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00147a027
更新日期：1992-08-18 00:00:00
abstract：:The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00331a025
更新日期：1985-05-07 00:00:00
abstract：:Several phosphatidylinositol 4,5-bisphosphate (PtdInsP2)-regulated actin-binding proteins and most phosphoinositide-specific phospholipases C (PI-PLCs) comprise a basic amino acid motif (KxxxKxKK, where x denotes any amino acid), which was previously suggested to represent a PtdInsP2-binding site commonly present in t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00015a023
更新日期：1995-04-18 00:00:00
abstract：:From an evolutionary point of view, plant and animal ferritins arose from a common ancestor, but plant ferritin exhibits different features as compared with the animal analogue. One major difference is that the 4-fold channels naturally occurring in plant ferritin are hydrophilic, whereas the 4-fold channels in animal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500066m
更新日期：2014-04-15 00:00:00
abstract：:We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a025
更新日期：1986-11-18 00:00:00
abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00126a020
更新日期：1992-03-24 00:00:00
abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a005
更新日期：1995-12-26 00:00:00
abstract：:The dependence of calcineurin on Ca2+ for activity is the result of the concerted action of calmodulin, which increases the turnover rate of the enzyme and modulates its response to Ca2+ transients, and of calcineurin B, which decreases the Km of the enzyme for its substrate. The calmodulin-stimulated protein phosphat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00188a015
更新日期：1994-06-07 00:00:00
abstract：:During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981152y
更新日期：1998-09-01 00:00:00
abstract：:Differential scanning calorimetry and CD spectrometry were employed to study the thermal unfolding of light meromyosin (LMM) prepared from carp acclimated to different temperatures. The transition temperatures given by the major peaks at pH 8.0 in 0.6 M KCl for LMM from carp acclimated to 10 degrees C were 32.5 and 39...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9701181
更新日期：1997-07-29 00:00:00
abstract：:Ribosomal protein E-L30 unfolds reversibly at pH values between 7.0 and 4.5. Unfolding of the protein involves a fast and a slow equilibrium, which depend on the degree of protonation of His19 and His33. Both the fast equilibrium between protonated and deprotonated histidines and the slow equilibrium between folded an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00391a050
更新日期：1987-08-25 00:00:00
abstract：:The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of four polypeptide chains which form a tetrameric unit. N-terminal residues, previously defined as a random coil or extended loop region,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00193a025
更新日期：1994-07-12 00:00:00
abstract：:AppA, a transcriptional antirepressor, regulates the steady expression of photosynthesis genes in Rhodobacter sphaeroides in response to high-intensity blue light and to redox signals. Its blue-light sensing is mediated by an N-terminal BLUF domain, a member of a novel flavin fold. The photocycle of this domain (AppA(...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051367p
更新日期：2006-01-10 00:00:00
abstract：:The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700426q
更新日期：2007-08-21 00:00:00
abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952339h
更新日期：1996-04-02 00:00:00
abstract：:The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi5008386
更新日期：2015-01-20 00:00:00
abstract：:Upon blue light irradiation, photoactive yellow protein (PYP) undergoes a conformational change that involves large movements at the N-terminus of the protein. We reasoned that this conformational change might be used to control other protein or peptide sequences if these were introduced as linkers connecting the N- a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400018h
更新日期：2013-05-14 00:00:00
abstract：:The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00241a014
更新日期：1991-07-09 00:00:00
abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992811p
更新日期：2000-06-13 00:00:00
abstract：:Tyrosine hydroxylase (TH) catalyzes the first step in the biosynthesis of catecholamines. Regulation of TH enzyme activity is controlled through the posttranslational modification of its regulatory domain. The regulatory domain of TH can be phosphorylated at four serines (8, 19, 31, and 40) by a variety of protein kin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7019468
更新日期：2008-02-12 00:00:00
abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a030
更新日期：1975-09-23 00:00:00
abstract：:Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01111
更新日期：2016-02-02 00:00:00
abstract：:Low pH-induced membrane insertion by diphtheria toxin T domain is crucial for A chain translocation into the cytoplasm. To define the membrane topography of the T domain, the exposure of biotinylated Cys residues to the cis and trans bilayer surfaces was examined using model membrane vesicles containing a deeply inser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049354j
更新日期：2004-07-20 00:00:00
abstract：:Chemical derivatization studies have been directed at the sulfhydryl group of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme. Reaction with N-ethylmaleimide leads to progressive and parallel loss of both enzymic activity and coenzyme binding. Both functions are lost when 1 equiv of sulfhydryl is deriva...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00564a021
更新日期：1980-11-11 00:00:00
abstract：:Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00312a024
更新日期：1984-08-14 00:00:00
abstract：:For many decades, protein folding experimentalists have worked with no information about the time scales of relevant protein folding motions and without methods for estimating the height of folding barriers. Protein folding experiments have been interpreted using chemical models in which the folding process is charact...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi060643c
更新日期：2006-07-18 00:00:00
abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00720
更新日期：2021-01-26 00:00:00
