Abstract:
:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of the manganese enzyme with L-Met-(S)-sulfoximine at pH 7. Dissociation of enzyme complexes containing an ADP analogue, L-Met-(S)-sulfoximine phosphate, and 2 equiv of Mn2+ is negligible at neutral pH. Prior to binding of the mercapto nucleotides to active sites, 6-mercaptopurine ribonucleoside triphosphate (6-S-ATP) and 8-mercaptoadenosine 5'-triphosphate (8-S-ATP) also have been further modified with fluorescent and chromogenic probes for energy-transfer measurements [Maurizi, M. R., Kasprzyk, P. G., & Ginsburg, A. (1986) Biochemistry (following paper in this issue)] or with electron-dense markers for electron microscopic and X-ray crystallographic structural analyses. Binding 6-S-ATP or 8-S-ATP to enzyme active sites at pH 7.1 produced red shifts of approximately 6 nm in nucleotide spectra characteristic for transfer of these nucleotide analogues into more acidic and hydrophobic environments. The spectrum of 6-S-ADP at active sites was more red-shifted than that of 6-S-AMP attached to adenylylation sites. The thiol group at the 6- or 8-position of the purine ring of the bound nucleotides was accessible for reactions with alkylating or mercurial reagents. Alkylation or mercaptide formation produced large blue shifts in the spectrum of enzyme-bound 6-S-ADP or 8-S-ADP at active sites or of 6-S-AMP covalently bound at adenylylation sites. At least one of two tryptophanyl residues in each subunit is very near the nucleotide binding site, as evidenced by changes in tryptophanyl residue fluorescence on binding ATP, mercaptonucleotides, or other ATP analogues.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Maurizi MR,Ginsburg Adoi
10.1021/bi00349a020subject
Has Abstractpub_date
1986-01-14 00:00:00pages
131-40issue
1eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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