Abstract:
:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal the binding mode of four different metal cyanides to bovine liver rhodanese: Na[Au(CN2], K2[Pt(CN)4], K2[Ni(CN)4], and K2[Zn(CN)4]. It appears that these complexes bind at one common site at the entrance of the active site pocket, interacting with the positively charged side chains of Arg-186 and Lys-249. This observation explains the inhibition of rhodanese by this class of compounds. For the platinum and nickel cyanide complexes virtually no other binding sites are observed. The gold complex binds, however, to three additional cysteine residues, thereby also displacing the extra sulfur atom which was bound to the essential Cys-247 in the sulfur-rhodanese complex. The zinc complex binds to completely different additional sites and forms complexes with the side chains of Asp-101 and His-203. Possible reasons for these different binding modes are discussed in terms of the preference for "hard" and "soft" ligands of these four metal ions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Lijk LJ,Kalk KH,Brandenburg NP,Hol WGdoi
10.1021/bi00281a026subject
Has Abstractpub_date
1983-06-07 00:00:00pages
2952-7issue
12eissn
0006-2960issn
1520-4995journal_volume
22pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The objective of this study was to determine the molecular factors that lead to beta-lactamase inhibitor resistance for the M69V variant in SHV-1 beta-lactamase. With mechanism-based inhibitors, the beta-lactamase forms an acyl-enzyme intermediate that consists of a trans-enamine derivative in the active site. This st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060990m
更新日期:2006-10-03 00:00:00
abstract::In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00010a025
更新日期:1995-03-14 00:00:00
abstract::Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01154
更新日期:2019-02-12 00:00:00
abstract::The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060421e
更新日期:2006-09-26 00:00:00
abstract::The use of cefoxitin, a poor substrate of the RTEM beta-lactamase, has allowed the kinetic and spectroscopic characterization of a covalent acyl-enzyme intermediate in the enzyme-catalyzed reaction. The rate of reappearance of catalytic activity in an enzyme sample diluted from an incubation with cefoxitin is nearly i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00554a012
更新日期:1980-06-24 00:00:00
abstract::The fetal Alz-50 reactive clone 1 (FAC1) protein exhibits altered expression and subcellular localization during neuronal development and neurodegenerative diseases such as Alzheimer's disease. Using the yeast two-hybrid screen, the human orthologue of Keap1 (hKeap1) was identified as a FAC1 interacting protein. Keap1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0494166
更新日期:2004-09-28 00:00:00
abstract::Oxalate is the strongest known inhibitor of yeast cytochrome b2 activity. We have used spectrophotometric titration, temperature-jump relaxation, and calorimetry in an investigation of the interaction between enzyme and inhibitor. The titration data are consistent with noncooperative binding to one site per subunit. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00647a002
更新日期:1976-01-27 00:00:00
abstract::The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00203a004
更新日期:1994-09-20 00:00:00
abstract::Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002938a
更新日期:2001-08-07 00:00:00
abstract::The two key structural features of alpha 2-macroglobulin (alpha 2M) involved in inhibitory caging of proteases are the thiol ester and the bait region. This paper examines the environment of the hydrolyzed thiol ester in methylamine-treated human alpha 2M and the separation between the bait region and the thiol ester ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00408a036
更新日期:1988-04-19 00:00:00
abstract::Various 6-alkylubiquinone or 6-(omega-haloalkyl)ubiquinone derivatives were synthesized through a radical coupling reaction between alkanoyl or omega-haloalkanoyl peroxides and ubiquinone 0. The latter was synthesized from 2-methoxy-4-methylphenol via nitration, methylation, reduction, and oxidation by modifications o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00260a028
更新日期:1982-08-17 00:00:00
abstract::We have developed a model of glycophorin in a phospholipid bilayer membrane in order to study the thermodynamics of this system and to understand the detailed behavior of recent calorimetric data. We assume that the larger glycophorin polar group can be considered as either adopting a pancakelike conformation at the b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00381a019
更新日期:1987-04-07 00:00:00
abstract::The tiny picoalga, Ostreococcus tauri, originating from the Thau Lagoon is a member of the marine phytoplankton. Because of its highly reduced genome and small cell size, while retaining the fundamental requirements of a eukaryotic photosynthetic cell, it became a popular model organism for studying photosynthesis or ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01200
更新日期:2019-04-09 00:00:00
abstract::Transferred nuclear Overhauser effect measurements (in the two-dimensional mode) have been used to determine the three-dimensional conformation of an ATP analogue, Co(NH3)4ATP, at the active site of sheep kidney Na,K-ATPase. Previous studies have shown that Co(NH3)4ATP is a competitive inhibitor with respect to MnATP ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a028
更新日期:1989-05-30 00:00:00
abstract::All CG dinucleotides along exons 5-8 of the p53 tumor suppressor gene contain endogenous 5-methylcytosine (MeC). These same sites (e.g., codons 157, 158, 245, 248, and 273) are mutational hot spots in smoking-induced lung cancer. Several groups used the UvrABC endonuclease incision assay to demonstrate that methylated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035259j
更新日期:2004-01-20 00:00:00
abstract::In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970160w
更新日期:1997-09-23 00:00:00
abstract::An empirical preduction algorithm which uses information on the shrs on either side) interactions in 20 proteins to assign every residue in a protein to one of five conformational states is described. The conformational states are defined in terms of the backbone dihedral angles of the residue so that the prediction a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00668a030
更新日期:1976-11-16 00:00:00
abstract::Serine hydroxymethyltransferase (SHMT) catalyzes the reversible conversion of l-serine and tetrahydrofolate into glycine and 5,10-methylenetetrahydrofolate. This enzyme, which plays a pivotal role in one-carbon metabolism, is involved in cancer metabolic reprogramming and is a recognized target of chemotherapy interve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01074
更新日期:2018-12-26 00:00:00
abstract::Interconversion of protein conformations is imperative to function, as evidenced by conformational changes associated with enzyme catalytic cycles, ligand binding and post-translational modifications. In this study, we used 15N NMR relaxation experiments to probe the fast (i.e., ps-ns) and slow (i.e., micros-ms) confo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701425n
更新日期:2007-12-25 00:00:00
abstract::A highly active, large-scale preparation of ubiquinol:cytochrome c2 oxidoreductase (EC 1.10.2.2; cytochrome bc1 complex) has been obtained from Rhodobacter sphaeroides. The enzyme was solubilized from chromatophores by using dodecyl maltoside in the presence of glycerol and was purified by anion-exchange and gel filtr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00463a004
更新日期:1990-03-20 00:00:00
abstract::Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00464a015
更新日期:1990-03-27 00:00:00
abstract::It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00118a014
更新日期:1992-01-28 00:00:00
abstract::The human immunodeficiency virus utilizes its gp41 fusion protein to mediate virus-cell membrane fusion. The conserved disulfide loop region in the gp41 hairpin conformation reverses the protein chain, such that the N-terminal heptad repeat and the C-terminal heptad repeat regions interact to form the six-helix bundle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300868f
更新日期:2012-09-04 00:00:00
abstract::The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00334a003
更新日期:1985-06-18 00:00:00
abstract::We have previously shown functional differences in fibrinogen from hyperhomocysteinemic rabbits compared to that in control rabbits. This acquired dysfibrinogenemia is characterized by fibrin clots that are composed of abnormally thin, tightly packed fibers with increased resistance to fibrinolysis. Homocysteine thiol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052076j
更新日期:2006-02-28 00:00:00
abstract::Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011929e
更新日期:2002-04-02 00:00:00
abstract::The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002926p
更新日期:2001-05-08 00:00:00
abstract::The reversible inhibition of beta-lactamase I from Bacillus cereus by cloxacillin, methicillin, and nafcillin has been systematically investigated. For these substrates the enzymatic reaction involves partitioning of the substrate between turnover and inhibition. Typically, concentrations of several hundred millimolar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00388a011
更新日期:1987-07-14 00:00:00
abstract::Unfolded apomyoglobin in 8 M urea at pH 2.3 displays distinct regions with different backbone mobility, as monitored by NMR relaxation. These variations in backbone mobility can be correlated with intrinsic properties of the amino acids in the sequence. Clusters of small amino acids such as glycine and alanine show in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020381o
更新日期:2002-10-22 00:00:00
abstract::The enhancement of the longitudinal proton relaxation rate of solvent water protons which occurs when Mn(II) is bound to the "tight" metal ion site of unadenylylated glutamine synthetase (GS) was used to determine the binding constant of L-methionine (SR)-sulfoximine to GS-Mn(II) complexes. The binary enhancement for ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00648a014
更新日期:1976-02-10 00:00:00