Modification of fibrinogen by homocysteine thiolactone increases resistance to fibrinolysis: a potential mechanism of the thrombotic tendency in hyperhomocysteinemia.

abstract：:Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...

journal_title：Biochemistry

authors： Bielecki M,Howe GW,Kluger R

更新日期：2019-08-27 00:00:00

abstract：:Although the binding of bivalent metal-ion activators to phosphoglucomutase produces substantial changes in the near ultraviolet spectrum of the enzyme, the extent to which aromatic residues are exposed to the aqueous environment, as assessed by means of solvent perturbation spectroscopy (using D2O), does not appear t...

journal_title：Biochemistry

authors： Ray WJ Jr

更新日期：1978-04-18 00:00:00

abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...

journal_title：Biochemistry

authors： Smith BC,Denu JM

更新日期：2007-12-18 00:00:00

abstract：:Multiple forms of the acute-phase serum protein SAA were isolated from the lipoprotein fraction of plasma from a single individual. These protein forms were purified by size-exclusion, ion-exchange, and reverse-phase high-pressure liquid chromatography, and then the tryptic peptides were subjected to amino acid sequen...

journal_title：Biochemistry

authors： Dwulet FE,Wallace DK,Benson MD

更新日期：1988-03-08 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:Unoccupied estrogen receptor (ER) can be extracted from tissues by homogenization with a hypotonic buffer, whereas hormone-occupied ER becomes tightly bound to the nuclear pellet and must be extracted with high-salt-containing buffers. The molecular basis for estrogen-induced tight nuclear binding of ER remains an imp...

journal_title：Biochemistry

authors： Fritsch M,Aluker M,Murdoch FE

更新日期：1999-06-01 00:00:00

abstract：:The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...

journal_title：Biochemistry

authors： Benson TE,Marquardt JL,Marquardt AC,Etzkorn FA,Walsh CT

更新日期：1993-03-02 00:00:00

abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...

journal_title：Biochemistry

authors： Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

更新日期：2005-04-12 00:00:00

abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...

journal_title：Biochemistry

authors： Da Silva GF,Harrison JS,Lai JR

更新日期：2010-07-06 00:00:00

abstract：:Previous studies have shown that protein 4.1 is a multifunctional protein that binds to spectrin, actin, glycophorins, the anion channel protein, and phosphatidylserine (PS). In this report, we have characterized the binding of protein 4.1 and its major proteolytic fragments to phospholipid vesicles. Pure 125I-labeled...

journal_title：Biochemistry

authors： Cohen AM,Liu SC,Lawler J,Derick L,Palek J

更新日期：1988-01-26 00:00:00

abstract：:The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one a...

journal_title：Biochemistry

authors： Poulos TL,Finzel BC,Howard AJ

更新日期：1986-09-09 00:00:00

abstract：:Accumulation of the cytotoxic 40- to 42-residue beta-amyloid peptide represents the primary pathological process in Alzheimer's disease (AD). BACE1 (beta-site APP cleaving enzyme 1) is responsible for the initial required step in the neuronal amyloidogenic processing of beta-amyloid precursor protein and is a major dr...

journal_title：Biochemistry

authors： Wang YS,Beyer BM,Senior MM,Wyss DF

更新日期：2005-12-20 00:00:00

abstract：:The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...

journal_title：Biochemistry

authors： Barkley MD,Lewis PA,Sullivan GE

更新日期：1981-06-23 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...

journal_title：Biochemistry

authors： Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

更新日期：1994-04-05 00:00:00

abstract：:The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...

journal_title：Biochemistry

authors： Lushnikov AY,Potaman VN,Oussatcheva EA,Sinden RR,Lyubchenko YL

更新日期：2006-01-10 00:00:00

abstract：:The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...

journal_title：Biochemistry

authors： Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

更新日期：1995-10-03 00:00:00

abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...

journal_title：Biochemistry

authors： Nagpal A,Valley MP,Fitzpatrick PF,Orville AM

更新日期：2006-01-31 00:00:00

abstract：:The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP co...

journal_title：Biochemistry

authors： Stehle R,Lionne C,Travers F,Barman T

更新日期：2000-06-27 00:00:00

abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

journal_title：Biochemistry

authors： Campisi D,Morii T,Barton JK

更新日期：1994-04-12 00:00:00

abstract：:Virulent strains of Clostridium sordellii cause gangrenous myonecrosis in humans. The released lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are regarded as the major virulence factors. TcsL inactivates low molecular weight GTP-binding proteins of the Rho/Ras subfamilies by monoglucosylation. In cultured cell lines...

journal_title：Biochemistry

authors： Schulz F,Just I,Genth H

更新日期：2009-09-29 00:00:00

abstract：:Human DNA polymerase kappa (pol kappa) has a sequence significantly homologous with that of Escherichia coli DNA polymerase IV (pol IV). We used a truncated form of human pol kappa (pol kappaDeltaC) and full-length pol IV to explore the miscoding properties of these enzymes. Oligodeoxynucleotides, modified site-specif...

journal_title：Biochemistry

authors： Suzuki N,Ohashi E,Hayashi K,Ohmori H,Grollman AP,Shibutani S

更新日期：2001-12-18 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00

abstract：:PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...

journal_title：Biochemistry

authors： Gama SR,Lo BSY,Séguin J,Pallitsch K,Hammerschmidt F,Zechel DL

更新日期：2019-12-31 00:00:00

abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...

journal_title：Biochemistry

authors： Ebneth A,Schweers O,Thole H,Fagin U,Urbanke C,Maass G,Wolfes H

更新日期：1994-12-06 00:00:00

abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

journal_title：Biochemistry

authors： Wagner PD,Stone DB

更新日期：1983-03-15 00:00:00

abstract：:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...

journal_title：Biochemistry

authors： Zimányi L,Lanyi JK

更新日期：1989-06-13 00:00:00

abstract：:The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69. Methylation of Cys 69 increases the sequence-specific DNA-binding activity of Ada by 10(3)-fold, enabling the methylated protein to activate transcription of a meth...

journal_title：Biochemistry

authors： Sun LJ,Yim CK,Verdine GL

更新日期：2001-09-25 00:00:00

abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...

journal_title：Biochemistry

authors： Addington AK,Johnson DA

更新日期：1996-10-22 00:00:00

abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

journal_title：Biochemistry

authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

更新日期：1994-11-01 00:00:00