Abstract：
:Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted by two members of this family, viz., temporin B (LLPIVGNLLKSLL-NH(2)) and temporin L (FVQWFSKFLGRIL-NH(2)). More specifically, we measured their insertion into lipid monolayers as well as their effects on the structural dynamics of liposomal bilayers as revealed by diphenylhexatriene (DPH)- and pyrene-labeled phospholipids. We also observed the impact of these peptides on the topology of giant vesicles. Both temporins readily penetrate into lipid monolayers, their intercalation being enhanced in the presence of the common bacterial negatively charged phospholipid phosphatidylglycerol. Instead, the eukaryotic lipid cholesterol did to some extent counteract their penetration into the lipid films. Both temporin B and temporin L caused an enrichment of phospholipids in the bilayers, and in the presence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), these peptides increased acyl chain order. Temporin B had practically no effect on giant liposomes composed of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), whereas rapid vesiculation was observed when POPG was present. In contrast, temporin L induced vesiculation of both SOPC and SOPC/POPG giant vesicles while the presence of cholesterol in SOPC giant vesicles attenuated this effect.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zhao H,Rinaldi AC,Di Giulio A,Simmaco M,Kinnunen PKdoi
10.1021/bi011929esubject
Has Abstractpub_date
2002-04-02 00:00:00pages
4425-36issue
13eissn
0006-2960issn
1520-4995pii
bi011929ejournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992253e
更新日期：2000-02-29 00:00:00
abstract：:CD23/Fc epsilonRII, the low-affinity receptor for IgE, is a multifunctional protein of importance in blood cell development and the immune system. We have studied the interaction of CD23 with IgE in solution using hydrodynamic methods applied to recombinant fragments of both ligands: sCD23, corresponding to the solubl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961231e
更新日期：1997-02-25 00:00:00
abstract：:The zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. Their mechanism is poorly understood, and there are no clinically useful inhibitors. While most members of the group contain two tightly bound zinc ions in their active site...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047709t
更新日期：2005-03-29 00:00:00
abstract：:Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00039a036
更新日期：1995-10-03 00:00:00
abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a018
更新日期：1992-07-21 00:00:00
abstract：:The navel orangeworm, Amyelois transitella (Walker), is an agricultural insect pest that can be controlled by disrupting male-female communication with sex pheromones, a technique known as mating disruption. Insect pheromone-binding proteins (PBPs) provide fast transport of hydrophobic pheromones through the aqueous s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9020132
更新日期：2010-02-23 00:00:00
abstract：:Structure-function studies of antibody-antigen systems include the identification of amino acid residues in the antigen that interact with an antibody and elucidation of their individual contributions to binding affinity. We used fluorescence correlation spectroscopy (FCS) and alanine-scanning mutagenesis to character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0607047
更新日期：2006-11-28 00:00:00
abstract：:The mechanism by which ATP binding transduces a conformational change in 70-kDa heat shock proteins that results in release of bound peptides remains obscure. Wei and Hendershot demonstrated that mutating Thr37 of hamster BiP to glycine impeded the ATP-induced conformational change, as monitored by proteolysis [(1995)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981510x
更新日期：1998-11-03 00:00:00
abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034394t
更新日期：2003-06-24 00:00:00
abstract：:Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols and halides. The reaction mechanism involves the formation of a covalent alkyl-enzyme complex which is hydrolyzed by water. The active site is a hydrophobic cavity buried between the main domain and the cap domain of the enzyme. The enzyme ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952904g
更新日期：1996-05-07 00:00:00
abstract：:The mechanism by which the contractile machinery of muscle is assembled and maintained is not well-understood. Members of the cysteine-rich protein (CRP) family have been implicated in these processes. Three vertebrate CRPs (CRP1-3) that exhibit developmentally regulated muscle-specific expression have been identified...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982036y
更新日期：1999-05-04 00:00:00
abstract：:Mammalian thioredoxin reductase (TR) is a pyridine nucleotide disulfide oxidoreductase that uses the rare amino acid selenocysteine (Sec) in place of the more commonly used amino acid cysteine (Cys) in the redox-active tetrapeptide Gly-Cys-Sec-Gly motif to catalyze thiol/disulfide exchange reactions. Sec can accelerat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400658g
更新日期：2014-02-04 00:00:00
abstract：:AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002766h
更新日期：2001-04-03 00:00:00
abstract：:When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00663a016
更新日期：1976-09-07 00:00:00
abstract：:Internal loops in RNA are important for folding and function. Consecutive noncanonical pairs can form in internal loops having at least two nucleotides on each side. Thermodynamic and structural insights into such internal loops should improve approximations for their stabilities and predictions of secondary and three...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052060t
更新日期：2006-03-28 00:00:00
abstract：:The RmFixL-RmFixJ oxygen signal transduction system ensures that a cascade of the Sinorhizobium meliloti nitrogen fixation genes is induced as the concentration of O2 drops below 50 microM in symbiotic nodules. Deoxy-RmFixL is a histidine protein kinase that catalyzes a phosphoryl transfer from ATP to the aspartate 54...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051661h
更新日期：2005-11-22 00:00:00
abstract：:Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9805339
更新日期：1998-06-09 00:00:00
abstract：:Assignments have been made for 11 methyl, one Gln-C gamma, one Thr-C beta, and all six Tyr-C zeta carbon resonances of ribonuclease A. These partially serve to delineate the binding sites for Cu2+, Mn2+, phosphate, cytidine and its 2'-, 3'-, and 5'-phosphates (Cyd and Cyd-2'-P, -3'-P, and -5'-P), and one or a few urea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a020
更新日期：1984-07-17 00:00:00
abstract：:Glycogen phosphorylase is a muscle enzyme which metabolizes glycogen, producing glucose-1-phosphate, which can be used for the production of ATP. Phosphorylase activity is regulated by phosphorylation/dephosphorylation, and by the allosteric binding of numerous effectors. In this work, we have studied 10 site-directed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0020372
更新日期：2001-05-01 00:00:00
abstract：:The properties of the high-affinity single-stranded DNA (ssDNA) binding state of Escherichia coli recA protein have been studied. We find that all of the nucleoside triphosphates that are hydrolyzed by recA protein induce a high-affinity ssDNA binding state. The effect of ATP binding to recA protein was partially sepa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a021
更新日期：1988-02-23 00:00:00
abstract：:As part of a search for organic compounds that selectively target RNA, we found that specific diphenylfuran derivatives, which are related to compounds that bind to the DNA minor groove, bind very strongly to RNA in a manner very sensitive to the structure of the compounds. In extended development of the diphenylfuran...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002338b
更新日期：2001-02-06 00:00:00
abstract：:Six active site mutants of Escherichia coli phosphofructokinase have been constructed and characterized using steady-state kinetics. All but one of the mutants (ES222) have significantly lower maximal activity, implicating these residues in the catalytic process. Replacement of Asp127, the key catalytic residue in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00153a017
更新日期：1992-09-29 00:00:00
abstract：:We have examined the phase partition preferences of the even chain length (n = 10-22) diacyl-3'3'-indocarbo-cyanine iodides (Cn diI) incorporated in disaturated lecithin (PC) vesicles. Two parameters were used to determine this phase preference: (i) the direction of shift of the phase transition temperature (Tm) induc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00567a039
更新日期：1980-12-23 00:00:00
abstract：:Dehaloperoxidase (DHP), discovered in the marine terebellid polychaete Amphitrite ornata, is the first heme-containing globin with a peroxidase activity. The sequence and crystal structure of DHP argue that it evolved from an ancient O(2) transport and storage globin. Thus, DHP retains an oxygen carrier function but a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2009129
更新日期：2011-09-27 00:00:00
abstract：:The catalytic activity of protein-tyrosine phosphatases (PTPs) is mediated by a cysteine side chain which carries out a nucleophilic attack initiating the phosphate cleavage. Experimentally, it has been observed that the active site cysteine has a remarkably low pKa. In the present study, we have investigated the orig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971187i
更新日期：1998-04-21 00:00:00
abstract：:Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061632m
更新日期：2006-12-26 00:00:00
abstract：:The cystic fibrosis transmembrane conductance regulator (CFTR) functions in vivo as a cAMP-activated chloride channel. A member of the ATP-binding cassette superfamily of membrane transporters, CFTR contains two transmembrane domains (TMDs), two nucleotide-binding domains (NBDs), and a regulatory (R) domain. It is pre...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035382a
更新日期：2004-02-03 00:00:00
abstract：:Inheritance of apoE4 is a strong risk factor for the development of late-onset sporadic Alzheimer's disease (AD). Several lines of evidence suggest that apoE4 binds to the Alzheimer Abeta protein and, under certain experimental conditions, promotes formation of beta-sheet structures and amyloid fibrils. Deposition of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0114269
更新日期：2001-12-11 00:00:00
abstract：:The distances between the anticodon loops of fluorescent tRNAPhe bound to the E site and to either the A or the P site of poly(U)-programmed Escherichia coli ribosomes were measured by fluorescence energy transfer. Donor and acceptor molecules were wybutine and proflavin, respectively, both located 3' to the anticodon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a002
更新日期：1986-05-20 00:00:00
abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00367a061
更新日期：1986-09-23 00:00:00
