Abstract:
:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes of helix formation associated with replacing AU pairs with GU mismatches are less than previously realized. At both 25 and 37 degrees C, the decrease in helix stability associated with replacing an AU with a GU is also less than thought previously. Approximations are suggested for predicting the effects of GU mismatches on helix stability.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sugimoto N,Kierzek R,Freier SM,Turner DHdoi
10.1021/bi00367a061subject
Has Abstractpub_date
1986-09-23 00:00:00pages
5755-9issue
19eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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