Abstract：
:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing. The secondary structure elements of GH1, three helices (S5-A16, S24-A34, N42-K56) followed by a beta-hairpin (L59-L73), are folded in a manner very similar to the corresponding parts of the globular domain of chicken histone H5 (GH5) [Clore et al. (1987) EMBO J. 6, 1833-1842; Ramakrishnan et al. (1993) Nature 362, 219-223]. However, subtle differences are detected between the two structures and between the electrostatic potentials surrounding the molecules. The most important differences are located in the loop between the second and third helices, a region that could be responsible for the different affinity for DNA. The most positively charged regions are not found in exactly the same position in GH1 and GH5. Nevertheless, their location seems to agree with the model where nucleosome binding takes place through contact points located at one DNA terminus and close to the dyad axis of the nucleosome [Schwabe & Travers (1993) Curr. Biol. 3, 628-630].
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga Kdoi
10.1021/bi00203a004subject
Has Abstractpub_date
1994-09-20 00:00:00pages
11079-86issue
37eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a021
更新日期：1990-02-13 00:00:00
abstract：:The kinetics of tert-butyl isocyanide binding to the heme protein horseradish peroxidase (HRP) at 22 degrees C was examined on all time scales, from minutes to picoseconds, in aqueous borate buffer at pH 9.08. Unlike myoglobin (Mb) or hemoglobin, HRP shows two bimolecular ligand binding processes. For comparison, bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9518149
更新日期：1996-02-06 00:00:00
abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00131a034
更新日期：1992-04-28 00:00:00
abstract：:Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00323a021
更新日期：1985-01-15 00:00:00
abstract：:The binding of propidium to poly(dA).poly(dT) [poly(dA.dT)] and to poly[d(A-T)].poly[d(A-T)] [poly[d(A-T)2]] has been compared under a variety of solution conditions by viscometric titrations, binding studies, and kinetic experiments. The binding of propidium to poly[d(A-T)2] is quite similar to its binding to calf th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00336a029
更新日期：1985-07-16 00:00:00
abstract：:Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00060a009
更新日期：1993-03-09 00:00:00
abstract：:Protein L is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species Peptostreptococcus magnus. It has affinity for immunoglobulin (Ig) through interaction with framework structures in the variable Ig light chain domain. The Ig-binding activity is located to five homologous re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00251a008
更新日期：1994-11-29 00:00:00
abstract：:Efforts toward the development of RNA-based drug leads have been challenging because of the complexity and dynamic nature of RNA structures as therapeutic targets. The transactivation response (TAR) RNA and cognate Tat protein of HIV have long been recognized as promising antiviral targets, and recent works have ident...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00325
更新日期：2015-06-16 00:00:00
abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00392
更新日期：2015-06-30 00:00:00
abstract：:The transmission of regulatory signals between the alpha- and beta-subunits of the tryptophan synthase alpha 2 beta 2 complex from Salmonella typhimurium has been investigated by monitoring the luminescence properties of the enzyme in the presence and in the absence of the alpha-subunit ligand DL-alpha-glycerol 3-phos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00148a014
更新日期：1992-08-25 00:00:00
abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960790i
更新日期：1996-10-01 00:00:00
abstract：:Recently, we described the formation of a complex between liposomes and the unfolded protein rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1), which could be liberated and efficiently reactivated after treatment of the complex with detergents [Zardeneta, G., & Horowitz, P. M. (1992) Eur. J. Biochem. 210, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00213a025
更新日期：1993-12-21 00:00:00
abstract：:Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00298
更新日期：2019-08-27 00:00:00
abstract：:The intracellular level of beta-hydroxydecanoyl thio ester dehydrase, the product of the fabA gene of Escherichia coli, was increased by isolation of a putative promotor mutant (termed fabAup) or by molecular cloning of the wild-type fabA gene into plasmid pBR322. The fabAup and plasmid-carrying strains overproduced d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00294a032
更新日期：1983-12-06 00:00:00
abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi9020378
更新日期：2010-02-09 00:00:00
abstract：:We determined the magnitude of the bend induced in DNA by an adenine-thymine tract by measuring the rate of cyclization of DNA oligonucleotides containing phased A tracts. A series of linear multimers with 2-bp single-stranded ends, in which the (A.T)6 tracts are separated by CG2-3C sequences and are positioned 10 and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a027
更新日期：1990-05-01 00:00:00
abstract：:Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00240a017
更新日期：1991-07-02 00:00:00
abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991307h
更新日期：2000-03-14 00:00:00
abstract：:X-ray absorption spectroscopy has been performed on oriented photosystem II membrane particles isolated from spinach. Structural features of the tetranuclear Mn cluster and the orientation of the cluster with respect to the lipid bilayer were determined in both the S1 and S2 states of the Kok cycle. Variation of the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00198a042
更新日期：1994-08-16 00:00:00
abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9526948
更新日期：1996-04-23 00:00:00
abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025990a
更新日期：2002-10-15 00:00:00
abstract：:We obtained a novel carbohydrate-binding peptide having a helix-loop-helix scaffold from a random peptide library. The helix-loop-helix peptide library randomized at five amino acid residues was displayed on the major coat protein of a filamentous phage. Affinity selection with a ganglioside, Galbeta1-3GalNAcbeta1-4(N...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8000837
更新日期：2008-07-01 00:00:00
abstract：:Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a018
更新日期：1976-10-19 00:00:00
abstract：:Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox).sarcosine) that exhibits an intense ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0600852
更新日期：2006-05-16 00:00:00
abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0525223
更新日期：2006-03-21 00:00:00
abstract：:We describe an assay for detecting intramolecular triple-stranded DNA in living cells. The assay involves quantitative analysis of the differential photobinding of 4,5',8-trimethylpsoralen to 5'TA and 5'AT dinucleotides in a region of plasmid DNA containing the triplex-forming sequence (GA)7TA(GA)7. Psoralen photobind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00075a013
更新日期：1993-06-22 00:00:00
abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00294a028
更新日期：1983-12-06 00:00:00
abstract：:The FMN prosthetic group of flavocytochrome b2 or L-lactate dehydrogenase oxidizes lactate to pyruvate. The reducing equivalents are then transferred one by one, intramolecularly, to heme b2 and then to external acceptors. Substrate oxidation is thought to begin with abstraction of the substrate alpha-hydrogen as a pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9529519
更新日期：1996-07-02 00:00:00
abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012148g
更新日期：2002-03-12 00:00:00
abstract：:The aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii from Enterococcus faecium is an important microbial resistance determinant and a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. We report here the further characterization of this enzyme in terms of the kinetic mechanism of acetyl transfer and id...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034148h
更新日期：2003-06-03 00:00:00
