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Polymerization pattern of insulin at pH 7.0.

Abstract：

:Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-association of specified oligomeric species. The analysis procedure, based on closed solutions formed by summing infinite series, yields for each pattern a set of equilibrium constants, It is shown that a distinction between the possible patterns can be made by analyzing sedimentation equilibrium results obtained in a higher total concentration range (up to 4 g/1.) with insulin freed of zinc and proinsulin, account being taken of the composition dependence of activity coefficients. The favored pattern, which differs from that previously reported in the literature, involves the dimerization of monomeric insulin (mol wt 5734), governed by a dimerization constant of 11 X 10(4) M-1 and the isodesmic indefinite self-association of the dimer, described by an association constant of 1.7 X 10(4) M-1. This polymerization pattern is also shown to be consistent with the reaction boundary observed in sedimentation velocity experiments.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Jeffrey PD,Milthorpe BK,Nichol LW

doi

10.1021/bi00666a018

subject

Has Abstract

pub_date

1976-10-19 00:00:00

pages

4660-5

issue

21

eissn

0006-2960

issn

1520-4995

journal_volume

15

pub_type

杂志文章

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