Interaction of tRNAs and of phosphorothioate-substituted nucleic acids with an organomercurial. Probing the chemical environment of thiolated residues by affinity electrophoresis.

abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

journal_title：Biochemistry

authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

更新日期：1975-09-23 00:00:00

abstract：:Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...

journal_title：Biochemistry

authors： Olson MO,Kirstein MN,Wallace MO

更新日期：1990-06-19 00:00:00

abstract：:Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...

journal_title：Biochemistry

authors： Bryant RW Jr,Bentley R

更新日期：1976-11-02 00:00:00

abstract：:The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...

journal_title：Biochemistry

authors： Ho C,Shanmugasundararaj S,Miller KW,Malinowski SA,Cook AC,Slater SJ

更新日期：2008-09-09 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

journal_title：Biochemistry

authors： Degani H,Shaer A,Victor TA,Kaye AM

更新日期：1984-06-05 00:00:00

abstract：:A stable green heme was extracted from ferric cyanosulfmyoglobin after it had undergone an internal conversion reaction. After iron removal and conversion to the methyl ester, the resulting green porphyrin was purified by high-pressure liquid chromatography. Visible, 1H NMR, and mass spectrometric studies provided evi...

journal_title：Biochemistry

authors： Bondoc LL,Chau MH,Price MA,Timkovich R

更新日期：1986-12-30 00:00:00

abstract：:Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electr...

journal_title：Biochemistry

authors： Gao X,Wen X,Esser L,Quinn B,Yu L,Yu CA,Xia D

更新日期：2003-08-05 00:00:00

abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...

journal_title：Biochemistry

authors： Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

更新日期：2003-03-18 00:00:00

abstract：:Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...

journal_title：Biochemistry

authors： Surerus KK,Chen M,van der Zwaan JW,Rusnak FM,Kolk M,Duin EC,Albracht SP,Münck E

更新日期：1994-04-26 00:00:00

abstract：:The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...

journal_title：Biochemistry

authors： Rho H,Jones CN,Rose RB

更新日期：2010-11-30 00:00:00

abstract：:The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...

journal_title：Biochemistry

authors： Tong X,Zhu J,Ma Y,Chen X,Wu G,He F,Cao C,Wu H

更新日期：2007-10-09 00:00:00

abstract：:An assay was developed to study the spontaneous transfer and transbilayer movement (flip-flop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). The assay is base...

journal_title：Biochemistry

authors： Bai J,Pagano RE

更新日期：1997-07-22 00:00:00

abstract：:While within a human host the opportunistic pathogen Toxoplasma gondii relies heavily on glycolysis for its energy needs. Lactate dehydrogenase (LDH), the terminal enzyme in anaerobic glycolysis necessary for NAD(+) regeneration, therefore represents an attractive therapeutic target. The tachyzoite stage lactate dehyd...

journal_title：Biochemistry

authors： Kavanagh KL,Elling RA,Wilson DK

更新日期：2004-02-03 00:00:00

abstract：:Band 3, the anion transport protein of human erythrocyte membranes, can be transferred from cells to liposomes and from liposomes back to cell membranes, retaining function and native orientation. After incubation with cells, sonicated phosphatidylcholine vesicles bind a transmembrane protein that comigrates with band...

journal_title：Biochemistry

authors： Newton AC,Cook SL,Huestis WH

更新日期：1983-12-20 00:00:00

abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

journal_title：Biochemistry

authors： Cech CL,McClure WR

更新日期：1980-05-27 00:00:00

abstract：:Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins R1 and R2, each inactive alone. The R1 protein binds the ribonucleotide substrates while the R2 protein contains a binuclear iron center and a tyrosyl free radical, essential for activity. The crystal structures of the corresponding Esc...

journal_title：Biochemistry

authors： Rova U,Goodtzova K,Ingemarson R,Behravan G,Gräslund A,Thelander L

更新日期：1995-04-04 00:00:00

abstract：:Phosphorylation of G protein-coupled receptors (GPCRs) terminates their ability to couple with and activate G proteins by increasing their affinity for arrestins. Unfortunately, detailed information regarding how GPCRs interact with the kinases responsible for their phosphorylation is still limited. Here, we purified ...

journal_title：Biochemistry

authors： Jones Brunette AM,Sinha A,David L,Farrens DL

更新日期：2016-06-07 00:00:00

abstract：:Upon cleavage of the reactive thioester bonds (Cys-949-Glx-952) of tetrameric human alpha 2-macroglobulin (alpha 2M) by methylamine, one sulfhydryl group per alpha 2M subunit is exposed. These identical sulfhydryl group sites were labeled with the thiol-specific nitroxide spin-labels (1-oxy-2,2,5,5-tetramethyl-3-pyrro...

journal_title：Biochemistry

authors： Zhao BL,Musci G,Sugawara Y,Berliner LJ

更新日期：1988-07-12 00:00:00

abstract：:Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

更新日期：1987-08-25 00:00:00

abstract：:We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...

journal_title：Biochemistry

authors： Soto AM,Rentzeperis D,Shikiya R,Alonso M,Marky LA

更新日期：2006-03-07 00:00:00

abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...

journal_title：Biochemistry

authors： Chen T,Haigentz M Jr,Reisler E

更新日期：1992-03-24 00:00:00

abstract：:The bile acid cholate disrupts platelet plasma membrane vesicles and inactivates 5-hydroxytryptamine transport activity. The transporter appears not to be solubilized by this procedure, but remains associated with a high molecular weight aggregate. Merely removing cholate does not restore the vesicular structure or tr...

journal_title：Biochemistry

authors： Rudnick G,Nelson PJ

更新日期：1978-11-28 00:00:00

abstract：:Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...

journal_title：Biochemistry

authors： Boles JO,Cisneros RJ,Weir MS,Odom JD,Villafranca JE,Dunlap RB

更新日期：1991-11-19 00:00:00

abstract：:The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonat...

journal_title：Biochemistry

authors： O'Brien JR,Schuller DJ,Yang VS,Dillard BD,Lanzilotta WN

更新日期：2003-05-20 00:00:00

abstract：:By use of a mutational assay employing an octadecamer with a mismatch in the center, it is shown that the introduction of phosphorothioate groups near the 5'-end can protect the mismatch against degradation by the 5'-3'-exonuclease activity of Escherichia coli DNA polymerase I. An optimal level of protection is achiev...

journal_title：Biochemistry

authors： Ott J,Eckstein F

更新日期：1987-12-15 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

journal_title：Biochemistry

authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

更新日期：1984-08-28 00:00:00

abstract：:Heparin potentiates the mitogenic activity of FGF-1 by increasing the affinity for its receptor and by extending its biological half-life. During the course of labeling human FGF-1 with Na(125)I and chloramine T, it was observed that the protein lost its ability to bind to heparin. In contrast, bovine FGF-1 retained i...

journal_title：Biochemistry

authors： Patrie KM,Botelho MJ,Franklin K,Chiu IM

更新日期：1999-07-20 00:00:00

abstract：:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...

journal_title：Biochemistry

authors： Smith AM,Morrison WL,Milham PJ

更新日期：1983-03-29 00:00:00