Abstract：
:The enzymes in the alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily represent the largest class of non-heme iron oxidases and have important medical, ecological, and biotechnological roles. One such enzyme, taurine/alpha-ketoglutarate dioxygenase (TauD), catalyzes the conversion of 2-aminoethanesulfonate (taurine) to sulfite and aminoacetaldehyde while decomposing alphaKG to succinate and CO(2). This alphaKG dependent dioxygenase is expressed in Escherichia coli under sulfur starvation conditions and allows the cell to utilize taurine, and other similar sulfonates in the environment, as an alternative sulfur source. In this work, we report the structures of the apo and holo forms of TauD to 1.9 A resolution (R(cryst) = 21.2%, R(free) = 24.9%) and 2.5 A resolution (R(cryst) = 22.5%, R(free) = 27.8%), respectively. The models reported herein provide significant new insight into the substrate orientations at the active site and the conformational changes that are induced upon taurine binding. Furthermore, analysis of our crystallographic data coupled with reanalysis of the crystallographic model (resolution = 3.0 A, R(cryst) = 28.1, R(free) = 32.0) presented by Elkins et al. (Biochemistry (2002) 41, 5185-5192) reveals an alternative oligomeric arrangement for the enzyme that is consistent with the conserved primary and secondary structure elements of other alphaKG dependent dioxygenases.
journal_name
Biochemistryjournal_title
Biochemistryauthors
O'Brien JR,Schuller DJ,Yang VS,Dillard BD,Lanzilotta WNdoi
10.1021/bi0341096subject
Has Abstractpub_date
2003-05-20 00:00:00pages
5547-54issue
19eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The amino acid histidine can play a significant role in the structure and function of proteins. Its various functions include enzyme catalysis, metal binding activity, and involvement in cation-π, π-π, salt-bridge, and other types of noncovalent interactions. Although histidine's imidazole nitrogens (Nδ and Nε) are kn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00253
更新日期：2016-07-12 00:00:00
abstract：:Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00323a021
更新日期：1985-01-15 00:00:00
abstract：:Ultrafast laser spectroscopy techniques are used to measure the low-frequency vibrational coherence spectra and nitric oxide rebinding kinetics of Caldariomyces fumago chloroperoxidase (CPO). Comparisons of the CPO coherence spectra with those of other heme species are made to gauge the protein-specific nature of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7025485
更新日期：2008-05-06 00:00:00
abstract：:The conformations of three peptide models of the one-disulfide and fully reduced forms of apamin were characterized by using nuclear magnetic resonance (NMR) spectroscopy. Apa-2 contains the native disulfide bond between Cys3 and Cys15 in apamin with the other two cysteines replaced by alanines. Apa-1 contains the nat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00183a031
更新日期：1994-05-03 00:00:00
abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101239r
更新日期：2011-01-25 00:00:00
abstract：:Artificial genetic systems have been developed by synthetic biologists over the past two decades to include additional nucleotides that form additional nucleobase pairs independent of the standard T:A and C:G pairs. Their use in various tools to detect and analyze DNA and RNA requires polymerases that synthesize duple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400558c
更新日期：2013-08-06 00:00:00
abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061184i
更新日期：2006-10-31 00:00:00
abstract：:Matrix metalloproteinase-13 (MMP-13) is important in the pathology of osteoarthritis (OA). Although MMP-13 is considered a therapeutic target for OA, it is unclear how MMP-13 activity is regulated by the system that comprises various proteinases and their inhibitors. MMP-13 neutralizing antibodies could be a useful to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301228d
更新日期：2012-11-06 00:00:00
abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9924258
更新日期：2000-06-20 00:00:00
abstract：:A well-defined region of pancreatic and other secreted phospholipase A2 (PLA2), which we call the i-face, makes a molecular contact with the interface to facilitate and control the events and processivity of the interfacial catalytic turnover cycles. The structural features of the i-face and its allosteric relationshi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9925470
更新日期：2000-05-16 00:00:00
abstract：:125I-Labeled enterotoxin from Clostridium perfringens was utilized to characterize the association of the enterotoxin with cells isolated from rabbit intestine and tissue homogenates from liver, kidney, and brain. The enterotoxin was found to bind in a specific and saturable manner to cells from intestine and to tissu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00562a014
更新日期：1980-10-14 00:00:00
abstract：:The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00492a005
更新日期：1990-10-09 00:00:00
abstract：:The Synechococcus PCC7942 SmtB is a zinc-responsive transcriptional repressor and a member of the ArsR superfamily of prokaryotic metalloregulatory transcription factors. The mechanism of negative regulation by Zn(II) and other metals as well as the coordination chemistry (stoichiometry, affinity, and specificity) of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001140o
更新日期：2000-09-26 00:00:00
abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a013
更新日期：1995-11-14 00:00:00
abstract：:Mycobacterial Ser/Thr kinase, PknB, is essential for the growth of the pathogen. Unphosphorylated PknB is catalytically inactive, and its activation requires autophosphorylation of Thr residues on the activation loop. Autophosphorylation can in principle take place via two distinct mechanisms. Intermolecular trans aut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500245v
更新日期：2014-07-22 00:00:00
abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982687u
更新日期：1999-03-09 00:00:00
abstract：:A photoaffinity probe for corticotropin (ACTH) receptors was prepared by selective modification of the single tryptophan residue in ACTH. A new photoreactive agent, 2,4-dinitro-5-azidophenylsulfenyl chloride, was synthesized and used for introducing the photoreactive group into ACTH. 2,4-Dinitro-5-azidophenylsulfenyl-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a027
更新日期：1980-07-08 00:00:00
abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1015474
更新日期：2011-03-08 00:00:00
abstract：:Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200978g
更新日期：2011-10-18 00:00:00
abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990286o
更新日期：1999-07-06 00:00:00
abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00026
更新日期：2015-06-30 00:00:00
abstract：:X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichrois...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00449a031
更新日期：1989-11-14 00:00:00
abstract：:One-carbon units are essential to a variety of anabolic processes which yield necessary cellular components including purines, pyrimidines, amino acids, and lipids. Serine hydroxymethyltransferase (SHMT) is the major provider of one-carbon units in the cell. The other product of this reaction is glycine. Both of these...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971610n
更新日期：1997-12-02 00:00:00
abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9904151
更新日期：1999-06-29 00:00:00
abstract：:Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphoryl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00448a013
更新日期：1989-10-31 00:00:00
abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a002
更新日期：1984-02-28 00:00:00
abstract：:Drosophila Rrp1 includes a carboxy-terminal region homologous to Escherichia coli exonuclease III which is sufficient to repair both oxidative and alkylation damage to DNA. An apurinic/apyrimidinic endonuclease activity intrinsic to Rrp1 was characterized previously. In this work, the 3'-phosphodiesterase and 3'-phosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00004a021
更新日期：1995-01-31 00:00:00
abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a024
更新日期：1988-05-17 00:00:00
abstract：:An inhibitor of the insulin receptor tyrosine kinase (IRTK), (hydroxy-2-naphthalenyl-methyl) phosphonic acid, was designed and synthesized and was shown to be an inhibitor of the biological effects of insulin in vitro. With a wheat germ purified human placental insulin receptor preparation, this compound inhibited the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a053
更新日期：1989-06-27 00:00:00
abstract：:Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602425c
更新日期：2007-09-18 00:00:00
