Location of sites in human lipocortin I that are phosphorylated by protein tyrosine kinases and protein kinases A and C.


:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping and sequence analysis. Lipocortin I was phosphorylated near the amino terminus at Tyr-21 by recombinant pp60c-src. The same tyrosine residue was phosphorylated by polyoma middle T/pp60c-src complex, by recombinant pp50v-abl, and with A431 cell membranes by the EGF receptor/kinase. The primary site of phosphorylation by protein kinase C was also near the amino terminus at Ser-27. The major site of phosphorylation by adenosine cyclic 3',5'-phosphate dependent protein kinase was on the carboxy-terminal half of the molecule at Thr-216. These sites are compared to the phosphorylation sites previously located in the structurally related protein lipocortin II.






Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB




Has Abstract


1988-05-17 00:00:00












  • Kinetic isotope effects on the oxidation of reduced nicotinamide adenine dinucleotide phosphate by the flavoprotein methylenetetrahydrofolate reductase.

    abstract::Previous work from this laboratory has established that the NADPH-menadione oxidoreductase reaction catalyzed by methylenetetrahydrofolate reductase from pig liver proceeds by Ping Pong Bi Bi kinetics and that the reductive half-reaction is rate limiting in steady-state turnover. We have now shown that methylenetetrah...


    pub_type: 杂志文章


    authors: Vanoni MA,Matthews RG

    更新日期:1984-10-23 00:00:00

  • Hydrophobic core fluidity of homologous protein domains: relation of side-chain dynamics to core composition and packing.

    abstract::The side-chain dynamics of methyl groups in two structurally related proteins from the fibronectin type III (fnIII) superfamily, the third fnIII domain from human tenascin (TNfn3) and the tenth fnIII domain from human fibronectin (FNfn10), have been studied by NMR spectroscopy. Side-chain order parameters reveal that ...


    pub_type: 杂志文章


    authors: Best RB,Rutherford TJ,Freund SM,Clarke J

    更新日期:2004-02-10 00:00:00

  • A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding.

    abstract::Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...


    pub_type: 杂志文章


    authors: Nienaber VL,Breddam K,Birktoft JJ

    更新日期:1993-11-02 00:00:00

  • Protoheme-carbon monoxide geminate kinetics.

    abstract::Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...


    pub_type: 杂志文章


    authors: Marden MC,Hazard ES 3rd,Gibson QH

    更新日期:1986-05-20 00:00:00

  • Functional role of the conserved active site proline of triosephosphate isomerase.

    abstract::The importance of the fully conserved active site proline, Pro168, for the reaction mechanism of triosephosphate isomerase (TIM) has been investigated by studying the enzymatic and crystallographic properties of the P168A variant of trypanosomal TIM. In TIM, Pro168 follows the key catalytic residue Glu167, situated at...


    pub_type: 杂志文章


    authors: Casteleijn MG,Alahuhta M,Groebel K,El-Sayed I,Augustyns K,Lambeir AM,Neubauer P,Wierenga RK

    更新日期:2006-12-26 00:00:00

  • Steady-state FTIR spectra of the photoreduction of QA and QB in Rhodobacter sphaeroides reaction centers provide evidence against the presence of a proposed transient electron acceptor X between the two quinones.

    abstract::In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...


    pub_type: 杂志文章


    authors: Breton J

    更新日期:2007-04-17 00:00:00

  • Site-specific epitope tagging of G protein-coupled receptors by bioorthogonal modification of a genetically encoded unnatural amino acid.

    abstract::We developed a general strategy for labeling expressed membrane proteins with a peptide epitope tag and detecting the tagged proteins in native cellular membranes. First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at various specific sites in a G protein-coupled receptor (GPCR), C-C ...


    pub_type: 杂志文章


    authors: Naganathan S,Ye S,Sakmar TP,Huber T

    更新日期:2013-02-12 00:00:00

  • Effect of structural modifications on the activity of the leadzyme.

    abstract::The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...


    pub_type: 杂志文章


    authors: Chartrand P,Usman N,Cedergren R

    更新日期:1997-03-18 00:00:00

  • Escherichia coli DNA-directed beta-galactosidase synthesis in presence and absence of Ca2+.

    abstract::DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...


    pub_type: 杂志文章


    authors: Jacobs KA,Schlessinger D

    更新日期:1977-03-08 00:00:00

  • Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light.

    abstract::Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a mea...


    pub_type: 杂志文章


    authors: Seawell PC,Simon TJ,Ganesan AK

    更新日期:1980-04-15 00:00:00

  • Treponema denticola PurE Is a bacterial AIR carboxylase.

    abstract::De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...


    pub_type: 杂志文章


    authors: Tranchimand S,Starks CM,Mathews II,Hockings SC,Kappock TJ

    更新日期:2011-05-31 00:00:00

  • The A-state of barnase.

    abstract::The acid-induced denaturation of barnase and its mutants has been analyzed to search for partly-folded intermediates. Differential scanning calorimetry of barnase deviates from two-state behavior below pH 4.0 at low ionic strength, with the maximum discrepancy at pH 2.7. Addition of 200 mM KCl apparently restores the ...


    pub_type: 杂志文章


    authors: Sanz JM,Johnson CM,Fersht AR

    更新日期:1994-09-20 00:00:00

  • Characterization of putative polyphosphoinositide binding motifs from phospholipase C beta 2.

    abstract::Several phosphatidylinositol 4,5-bisphosphate (PtdInsP2)-regulated actin-binding proteins and most phosphoinositide-specific phospholipases C (PI-PLCs) comprise a basic amino acid motif (KxxxKxKK, where x denotes any amino acid), which was previously suggested to represent a PtdInsP2-binding site commonly present in t...


    pub_type: 杂志文章


    authors: Simões AP,Reed J,Schnabel P,Camps M,Gierschik P

    更新日期:1995-04-18 00:00:00

  • Ethylene enhances reactivity of superoxide with peroxidase to form the oxy-ferrous complex.

    abstract::Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...


    pub_type: 杂志文章


    authors: Smith AM,Morrison WL,Milham PJ

    更新日期:1983-03-29 00:00:00

  • There is only one phosphoenzyme intermediate with bound calcium on the reaction pathway of the sarcoplasmic reticulum calcium ATPase.

    abstract::Identical first-order rate constants for phosphorylation of the calcium ATPase of sarcoplasmic reticulum by bound inorganic phosphate (Pi) of 25 +/- 2 s-1 with empty vesicles and 25 +/- 1 s-1 with vesicles that were passively loaded with 40 mM Ca2+ were obtained by treating the reaction as an approach to equilibrium (...


    pub_type: 杂志文章


    authors: Myung J,Jencks WP

    更新日期:1995-03-07 00:00:00

  • A heuristic approach to the analysis of enzymic catalysis: reaction of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-allylglycine catalyzed by isopenicillin N synthase isozymes.

    abstract::Isopenicillin N synthase (IPNS) catalyzes the oxidative cyclization of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine to isopenicillin N. It is proposed that the multiple products produced from certain substrate analogues result from pathway branching after formation of a ferryl oxene intermediate. We have been int...


    pub_type: 杂志文章


    authors: Blackburn JM,Sutherland JD,Baldwin JE

    更新日期:1995-06-06 00:00:00

  • Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits.

    abstract::Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...


    pub_type: 杂志文章


    authors: Matsui T,Mizuochi T,Titani K,Okinaga T,Hoshi M,Bousfield GR,Sugino H,Ward DN

    更新日期:1994-11-29 00:00:00

  • The sigma enigma: in vitro/in silico site-directed mutagenesis studies unveil σ1 receptor ligand binding.

    abstract::The σ1 receptor is an integral membrane protein that shares no homology with other receptor systems, has no unequivocally identified natural ligands, but appears to play critical roles in a wide variety of cell functions. While the number of reports of the possible functions of the σ1 receptor is increasing, almost no...


    pub_type: 杂志文章


    authors: Brune S,Schepmann D,Klempnauer KH,Marson D,Dal Col V,Laurini E,Fermeglia M,Wünsch B,Pricl S

    更新日期:2014-05-13 00:00:00

  • Heme-CO religation in photolyzed hemoglobin: a time-resolved Raman study of the Fe-CO stretching mode.

    abstract::Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...


    pub_type: 杂志文章


    authors: Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

    更新日期:1993-02-09 00:00:00

  • Structural effects in reactivity and adduct formation of polycyclic aromatic epoxide and diol epoxide derivatives with DNA: comparison between 1-oxiranylpyrene and benzo[a]pyrenediol epoxide.

    abstract::Reaction of 1-oxiranylpyrene (1-OP) with DNA and the structures of the covalent and noncovalent complexes formed were studied in aqueous media (5 mM phosphate buffer with 0.1 M NaCl, pH 7) by utilizing the techniques of absorption, fluorescence and linear dichroism spectroscopy in order to gain an understanding of pos...


    pub_type: 杂志文章


    authors: Kim MH,Geacintov NE,Pope M,Harvey RG

    更新日期:1984-11-06 00:00:00

  • Mannitol-specific carrier protein from the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system can be extracted as a dimer from the membrane.

    abstract::The association state of the mannitol-specific enzyme II (EIIMtl) has been studied both in the purified form and embedded in the cytoplasmic membrane. Membrane fragments obtained from mannitol-grown Escherichia coli catalyze the phosphoenolpyruvate- (PEP) dependent phosphorylation of both glucose and mannitol; thus th...


    pub_type: 杂志文章


    authors: Roossien FF,Robillard GT

    更新日期:1984-11-20 00:00:00

  • Distinct activation states of alpha5beta1 integrin show differential binding to RGD and synergy domains of fibronectin.

    abstract::alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...


    pub_type: 杂志文章


    authors: García AJ,Schwarzbauer JE,Boettiger D

    更新日期:2002-07-23 00:00:00

  • Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution.

    abstract::Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant ...


    pub_type: 杂志文章


    authors: Lauber T,Nourse A,Schulz A,Marx UC

    更新日期:2002-12-10 00:00:00

  • Kinetic analysis of product inhibition in human manganese superoxide dismutase.

    abstract::Manganese superoxide dismutase (MnSOD) cycles between the Mn(II) and Mn(III) states during the catalyzed disproportionation of O(2)(*-), a catalysis that is limited at micromolar levels of superoxide by a peroxide-inhibited complex with the metal. We have investigated the role in catalysis and inhibition of the conser...


    pub_type: 杂志文章


    authors: Hearn AS,Stroupe ME,Cabelli DE,Lepock JR,Tainer JA,Nick HS,Silverman DN

    更新日期:2001-10-09 00:00:00

  • Interaction of assembly protein AP-2 and its isolated subunits with clathrin.

    abstract::The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...


    pub_type: 杂志文章


    authors: Prasad K,Keen JH

    更新日期:1991-06-04 00:00:00

  • Amino acid Asp181 of 5'-flap endonuclease 1 is a useful target for chemotherapeutic development.

    abstract::DNA alkylation-induced damage is one of the most efficacious anticancer therapeutic strategies. Enhanced DNA alkylation and weakened DNA repair capacity in cancer cells are responsible for the effectiveness of DNA-alkylating therapies. 5'-Flap endonuclease 1 (Fen1) is an important enzyme involved in base excision repa...


    pub_type: 杂志文章


    authors: Panda H,Jaiswal AS,Corsino PE,Armas ML,Law BK,Narayan S

    更新日期:2009-10-27 00:00:00

  • Phospholipid asymmetry in large unilamellar vesicles induced by transmembrane pH gradients.

    abstract::The influence of membrane pH gradients on the transbilayer distribution of some common phospholipids has been investigated. We demonstrate that the transbilayer equilibrium of the acidic phospholipids egg phosphatidylglycerol (EPG) and egg phosphatidic acid (EPA) can be manipulated by membrane proton gradients, wherea...


    pub_type: 杂志文章


    authors: Hope MJ,Redelmeier TE,Wong KF,Rodrigueza W,Cullis PR

    更新日期:1989-05-16 00:00:00

  • Biochemical and biological characterization of neutrophil chemotactic protein, a novel rabbit CXC chemokine from alveolar macrophages.

    abstract::The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...


    pub_type: 杂志文章


    authors: Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

    更新日期:2000-11-28 00:00:00

  • A method for the separation of hybrids of chromatographically identical oligomeric proteins. Use of 3,4,5,6-tetrahydrophthaloyl groups as a reversible "chromatographic handle".

    abstract::Hybridization experiments with variants of an oligomeric protein often provide important information regarding subunit structure, function, and interactions. In some systems, however, the variants are so similar electrophoretically and chromatographically that purification of individual hybrids is not feasible. Theref...


    pub_type: 杂志文章


    authors: Gibbons I,Schachman HK

    更新日期:1976-01-13 00:00:00

  • Binding of Ca2+ to the calcium adenosinetriphosphatase of sarcoplasmic reticulum.

    abstract::The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...


    pub_type: 杂志文章


    authors: Petithory JR,Jencks WP

    更新日期:1988-11-15 00:00:00