Abstract:
:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis of RLF analogues with specific modifications in positions B27 and B25, or the truncated form des(B27-31)RLF, clearly indicate that the intact indole ring in position B27 is crucial for high RLF receptor-binding. Receptor-binding was reduced by 2 orders of magnitude for Leu(B27)RLF (3%), Ala(B27)RLF (2.1%), and des(B27-31)RLF (0.4%), whereas slightly better binding was observed for His(B27)RLF (7.5%), Phe(B27)RLF (21%), D-Trp(B27) (26%), and the oxindole(B27)RLF (41%). On the basis of these observation it seems that an aromatic ring system in the beta- or gamma-position is required for binding. Structure prediction of the C-terminal region of the B chain indicated a possible type I or type III turn for residues C-G-G-P-R (B22-26) preceding the tryptophan. Exchanging Pro(B25) for D-Pro within the turn caused a severe structural rearrangement at the C terminus and a 96% drop in activity. It appears that the steric effect of L-Pro(B25) is important for the proper positioning of Trp(B27).
journal_name
Biochemistryjournal_title
Biochemistryauthors
Büllesbach EE,Schwabe Cdoi
10.1021/bi982687usubject
Has Abstractpub_date
1999-03-09 00:00:00pages
3073-8issue
10eissn
0006-2960issn
1520-4995pii
bi982687ujournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a022
更新日期:1987-06-30 00:00:00
abstract::Antifreeze proteins (AFPs) inhibit the growth of ice by binding to the surface of ice crystals, preventing the addition of water molecules to cause a local depression of the freezing point. AFPs from insects are much more effective at depressing the freezing point than fish AFPs. Here, we have investigated the possibi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035605x
更新日期:2004-01-13 00:00:00
abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982063o
更新日期:1998-12-01 00:00:00
abstract::Cyclopropane fatty acid (CFA) synthase of Escherichia coli catalyzes a modification of the acyl chains of phospholipid bilayers. We report (i) identification of the CFA synthase protein, (ii) overproduction (> 600-fold) and purification to essential homogeneity of the enzyme, and (iii) the amino acid sequence of CFA s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a011
更新日期:1992-11-17 00:00:00
abstract::Isotope exchange studies show that beef liver fructokinase has a random kinetic mechanism in which release of fructose from the enzyme is slower than that catalytic reaction. The stickiness of fructose in the presence of MgATP is confirmed by isotope partition studies, which show it to be released 0.53 times as fast a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a021
更新日期:1977-05-17 00:00:00
abstract::We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960034c
更新日期:1996-05-21 00:00:00
abstract::The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00156a003
更新日期:1992-10-20 00:00:00
abstract::Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00520a035
更新日期:1981-08-18 00:00:00
abstract::A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00333a037
更新日期:1985-06-04 00:00:00
abstract::A recirculating Langendorff heart preparation is used to characterize the endogenous antithrombin associated reversibly with murine vascular endothelium. Rat hearts are perfused clear of blood and then recirculated with a physiological salt solution. Addition of heparin educes antithrombin activity continuously into t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00169a024
更新日期:1994-01-25 00:00:00
abstract::Kirromycin and related antibiotics inhibit protein synthesis in bacteria by acting on elongation factor Tu (EF-Tu). We have studied the effects of N-methylkirromycin (aurodox) on some molecular properties of this protein. The binding of the antibiotic causes a dramatic variation in the protein fluorescence emission sp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a046
更新日期:1989-08-22 00:00:00
abstract::In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035319l
更新日期:2004-01-13 00:00:00
abstract::The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00590a031
更新日期:1979-11-13 00:00:00
abstract::The Escherichia coli ATP-consuming chaperonin machinery, a complex between GroEL and GroES, has evolved to facilitate folding of substrate proteins (SPs) that cannot do so spontaneously. A series of kinetic experiments show that the SPs are encapsulated in the GroEL/ES nanocage for a short duration. If confinement of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00903
更新日期:2021-01-19 00:00:00
abstract::In Escherichia coli, the cyclic AMP receptor protein (CRP) serves as a sensor of the intracellular level of cyclic AMP. At increasing concentrations of cyclic AMP, CRP becomes activated upon binding a cyclic AMP molecule. The activated CRP is capable of regulating the expression of more than 20 genes by binding to spe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972450i
更新日期:1998-04-14 00:00:00
abstract::The resonance Raman spectra of yeast ferri- and ferro-iso-1-cytochrome c dissolved in H2O and D2O are reported. Hydrogen exchange in the protein leads to distinct spectral changes of heme vibrational bands, particularly in the region between 670 and 710 cm-1 and at approximately 443 and approximately 450 cm-1. The lat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00214a013
更新日期:1993-12-28 00:00:00
abstract::During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700416h
更新日期:2007-07-10 00:00:00
abstract::The high resolution proton nuclear magnetic resonance (NMR) spectra of the synthetic DNA block polymer d(C15A15)-d(T15G15) were studied in order to more completely understand telestability in DNA, and to provide fundamental NMR data on DNA helices and random coils. Spectra were measured in the spectral region from 0 t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00622a031
更新日期:1977-02-08 00:00:00
abstract::Hydrogenases are metalloenzymes that catalyze the reversible reduction of protons at unusual metal centers. This Current Topic discusses recent advances in elucidating the steps involved in the biosynthesis of the complex metal cluster at the [FeFe]-hydrogenase (HydA) active site, known as the H-cluster. The H-cluster...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500210x
更新日期:2014-07-01 00:00:00
abstract::In efforts to understand the mechanisms of many nucleic acid enzymes, the first site-directed mutations are made at conserved acidic active residues. Almost without exception, the low or null activities of the resulting variants are attributed to the importance of the acidic residue(s) to the ligation of required meta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000337d
更新日期:2000-09-05 00:00:00
abstract::7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026339a
更新日期:2002-10-22 00:00:00
abstract::High-resolution nitrite soaked oxidized and reduced crystal structures of two active site mutants, D98N and H255N, of nitrite reductase (NIR) from Alcaligenes faecalis S-6 were determined to better than 2.0 A resolution. In the oxidized D98N nitrite-soaked structures, nitrite is coordinated to the type II copper via i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0107400
更新日期:2001-08-07 00:00:00
abstract::In the putative secondary structure of the transposon Tn10-encoded metal-tetracycline/H+ antiporter [Yamaguchi, et al. (1992) J. Biol. Chem. 267, 7490-7498], Tyr50-XXX-Gln54 in transmembrane helix 2 and Gly80-XXX-Asp84 in helix 3 are thought to face each other in the N-terminal region. At the corresponding positions i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00072a027
更新日期:1993-06-01 00:00:00
abstract::Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0481895
更新日期:2004-12-07 00:00:00
abstract::Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00615a018
更新日期:1978-10-31 00:00:00
abstract::The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a003
更新日期:1991-09-17 00:00:00
abstract::A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00288a014
更新日期:1983-09-13 00:00:00
abstract::The structure and backbone dynamics of an extended second transmembrane segment (TM2e) of the human neuronal glycine receptor alpha(1) subunit in sodium dodecyl sulfate micelles were studied by (1)H and (15)N solution-state NMR. The 28-amino acid segment contained the consensus TM2 domain plus part of the linker betwe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026767g
更新日期:2003-04-08 00:00:00
abstract::To shed more light on the molecular requirements for recognition of thyroid response elements (TREs) by thyroid receptors (TRs), we compared the specific aspects of DNA TRE recognition by different TR constructs. Using fluorescence anisotropy, we performed a detailed and hierarchical study of TR-TRE binding. This was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901282s
更新日期:2010-02-09 00:00:00
abstract::Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01033
更新日期:2019-01-15 00:00:00