当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > [FeFe]-hydrogenase maturation.

[FeFe]-hydrogenase maturation.

Abstract：

:Hydrogenases are metalloenzymes that catalyze the reversible reduction of protons at unusual metal centers. This Current Topic discusses recent advances in elucidating the steps involved in the biosynthesis of the complex metal cluster at the [FeFe]-hydrogenase (HydA) active site, known as the H-cluster. The H-cluster is composed of a 2Fe subcluster that is anchored within the active site by a bridging cysteine thiolate to a [4Fe-4S] cubane. The 2Fe subcluster contains carbon monoxide, cyanide, and bridging dithiolate ligands. H-cluster biosynthesis is now understood to occur stepwise; standard iron-sulfur cluster assembly machinery builds the [4Fe-4S] cubane of the H-cluster, while three specific maturase enzymes known as HydE, HydF, and HydG assemble the 2Fe subcluster. HydE and HydG are both radical S-adenosylmethionine enzymes that interact with an iron-sulfur cluster binding GTPase scaffold, HydF, during the construction of the 2Fe subcluster moiety. In an unprecedented biochemical reaction, HydG cleaves tyrosine and decomposes the resulting dehydroglycine into carbon monoxide and cyanide ligands. The role of HydE in the biosynthetic pathway remains undefined, although it is hypothesized to be critical for the synthesis of the bridging dithiolate. HydF is the site where the complete 2Fe subcluster is formed and ultimately delivered to the immature hydrogenase protein in the final step of [FeFe]-hydrogenase maturation. This work addresses the roles of and interactions among HydE, HydF, HydG, and HydA in the formation of the mature [FeFe]-hydrogenase.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Shepard EM,Mus F,Betz JN,Byer AS,Duffus BR,Peters JW,Broderick JB

doi

10.1021/bi500210x

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

4090-104

issue

25

eissn

0006-2960

issn

1520-4995

journal_volume

53

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Evidence for a recombination-independent pathway for the repair of DNA interstrand cross-links based on a site-specific study with nitrogen mustard.

    abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962778w

    authors： Berardini M,Mackay W,Loechler EL

    更新日期：1997-03-25 00:00:00

  • Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1α .

    abstract：:The two disparate functions of DCoH1 (dimerization cofactor of HNF-1)/PCD (pterin-4a-carbinolamine dehydratase) are associated with a change in oligomeric state. DCoH dimers enhance the activity of the diabetes-associated transcription factor HNF-1α (hepatocyte nuclear factor-1α), while the PCD activity of DCoH1 homot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1015056

    authors： Rho H,Jones CN,Rose RB

    更新日期：2010-11-30 00:00:00

  • Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase.

    abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201500c

    authors： Marapakala K,Qin J,Rosen BP

    更新日期：2012-02-07 00:00:00

  • N1-(5'-phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase: purification and characterization of a unique metalloenzyme.

    abstract：:N1-(5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynthesis catalyzing the hydrolysis of the N1-C6 bond of the purine substrate, a reaction unique to this pathway. A source of the recombinant monofunctional Methanococcus vannielii PR-AMP c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982475x

    authors： D'Ordine RL,Klem TJ,Davisson VJ

    更新日期：1999-02-02 00:00:00

  • A bifunctional fusion protein containing Fc-binding fragment B of staphylococcal protein A amino terminal to antidigoxin single-chain Fv.

    abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00487a005

    authors： Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

    更新日期：1990-09-04 00:00:00

  • The imine-pyridine torsion of the pyridoxal 5'-phosphate Schiff base of aspartate aminotransferase lowers its pKa in the unliganded enzyme and is crucial for the successive increase in the pKa during catalysis.

    abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981517e

    authors： Hayashi H,Mizuguchi H,Kagamiyama H

    更新日期：1998-10-27 00:00:00

  • Carbon dioxide hydration activity of carbonic anhydrase in mixtures of water and deuterium oxide.

    abstract：:We have measured the hydration of CO2 catalyzed by bovine red cell carbonic anhydrase in mixtures of H2O and D2O at pH(D) which is in the plateau region of the pH--activity profile. The steady-state parameters for hydration Vmax and Km both show an exponential dependence on the atom fraction of deuterium in solvent wa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00563a008

    authors： Venkatasubban KS,Silverman DN

    更新日期：1980-10-28 00:00:00

  • Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.

    abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00754

    authors： Luo M,Tanner JJ

    更新日期：2015-09-08 00:00:00

  • Functional segregation of a predicted "hinge" site within the beta-strand linkers of Escherichia coli leucyl-tRNA synthetase.

    abstract：:Some aminoacyl-tRNA synthetases (AARSs) employ an editing mechanism to ensure the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS), isoleucyl-tRNA synthetase (IleRS), and valyl-tRNA synthetase (ValRS) share a common insertion, called the CP1 domain, which is responsible for clearing misformed products. Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702494q

    authors： Mascarenhas AP,Martinis SA

    更新日期：2008-04-22 00:00:00

  • Infrared spectroscopic study of a "hard-to-exchange" phospholipid population in the vicinity of CaATPase.

    abstract：:CaATPase from rabbit sarcoplasmic reticulum (SR) has been isolated, purified, and reconstituted into unilamellar vesicles with 1,2-dipentadecanoylphosphatidylcholine (DPePC) and acyl-chain-perdeuterated 1,2-dipalmitoylphosphatidylcholine (DPPC-d62). For total lipid:protein mole ratios between 25 and 70, a constant "ha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00075a024

    authors： Senak L,Mendelsohn R

    更新日期：1993-06-22 00:00:00

  • Solution structure of a designed spirocyclic helical ligand binding at a two-base bulge site in DNA.

    abstract：:The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neoca...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602599d

    authors： Zhang N,Lin Y,Xiao Z,Jones GB,Goldberg IH

    更新日期：2007-04-24 00:00:00

  • Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase.

    abstract：:This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048606d

    authors： Murkin AS,Chou WK,Wakarchuk WW,Tanner ME

    更新日期：2004-11-09 00:00:00

  • Thermodynamic characterization of nucleoplasmin unfolding: interplay between function and stability.

    abstract：:The unfolding equilibrium of recombinant (rNP) and natural variants of nucleoplasmin (NP) from Xenopus laevis has been analyzed using biochemical and spectroscopic techniques. In the presence of denaturing concentrations of guanidinium salts (GuHCl and GuSCN), both domains, core and tail, of the rNP pentamer unfold as...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8002555

    authors： Franco G,Bañuelos S,Falces J,Muga A,Urbaneja MA

    更新日期：2008-07-29 00:00:00

  • pH-responsive DNA-binding activity of Helicobacter pylori NikR.

    abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801742r

    authors： Li Y,Zamble DB

    更新日期：2009-03-24 00:00:00

  • Extension of the binding motif of the Sin3 interacting domain of the Mad family proteins.

    abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355645

    authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

    更新日期：2004-01-13 00:00:00

  • Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel.

    abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0477034

    authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

    更新日期：2005-04-26 00:00:00

  • Selection and characterization of a new class of peptide exosite inhibitors of coagulation factor VIIa.

    abstract：:A new series of peptide inhibitors of human Factor VIIa (FVIIa) has been identified and affinity matured from naive and partially randomized peptide phage libraries selected against the immobilized tissue factor x Factor VIIa (TF x FVIIa) complex. These "A-series" peptides contain a single disulfide bond and a 13-resi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010591l

    authors： Dennis MS,Roberge M,Quan C,Lazarus RA

    更新日期：2001-08-14 00:00:00

  • Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.

    abstract：:Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060455m

    authors： Brandt G,Carrasco N,Huang Z

    更新日期：2006-07-25 00:00:00

  • The stretching frequencies of bound alkyl isocyanides indicate two distinct ligand orientations within the distal pocket of myoglobin.

    abstract：:The FTIR spectra for alkyl isocyanides (CNRs) change from a single nu(CN) band centered at approximately 2175 cm(-1) to two peaks at approximately 2075 and approximately 2125 cm(-1) upon binding to sperm whale myoglobin (Mb). The low- and high-frequency peaks have been assigned to in and out conformations, respectivel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100172c

    authors： Blouin GC,Olson JS

    更新日期：2010-06-22 00:00:00

  • The role of serine 167 in human indoleamine 2,3-dioxygenase: a comparison with tryptophan 2,3-dioxygenase.

    abstract：:The initial step in the l-kynurenine pathway is oxidation of l-tryptophan to N-formylkynurenine and is catalyzed by one of two heme enzymes, tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO). Here, we address the role of the conserved active site Ser167 residue in human IDO (S167A and S167H variant...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702405a

    authors： Chauhan N,Basran J,Efimov I,Svistunenko DA,Seward HE,Moody PC,Raven EL

    更新日期：2008-04-22 00:00:00

  • Structural principles for the multispecificity of small GTP-binding proteins.

    abstract：:The functional diversity of small GTP-binding proteins (G proteins) and their ability to function as molecular switches are based on their interactions with many different proteins. A wealth of structural data has revealed that their partners are often unrelated to each other in sequence and structure, but their bindi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049630u

    authors： Biou V,Cherfils J

    更新日期：2004-06-08 00:00:00

  • Cloning of a complementary deoxyribonucleic acid encoding a portion of rat intestinal preapolipoprotein AIV messenger ribonucleic acid.

    abstract：:Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00265a007

    authors： Gordon JI,Smith DP,Alpers DH,Strauss AW

    更新日期：1982-10-26 00:00:00

  • RNA species that replicate with DNA-dependent RNA polymerase from Escherichia coli.

    abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002756g

    authors： Wettich A,Biebricher CK

    更新日期：2001-03-20 00:00:00

  • Cell-surface-associated tissue transglutaminase is a target of MMP-2 proteolysis.

    abstract：:MT1-MMP, a prototypic member of a membrane-type metalloproteinase subfamily, is an invasion promoting protease and an activator of MMP-2. In addition, MT1-MMP proteolysis regulates the functionality of cell-surface adhesion/signaling receptors including tissue transglutaminase (tTG). tTG is known to serve as an adhesi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049266z

    authors： Belkin AM,Zemskov EA,Hang J,Akimov SS,Sikora S,Strongin AY

    更新日期：2004-09-21 00:00:00

  • The crystal structure of the C-terminal truncated apolipoprotein A-I sheds new light on amyloid formation by the N-terminal fragment.

    abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi2017014

    authors： Gursky O,Mei X,Atkinson D

    更新日期：2012-01-10 00:00:00

  • Heterodimerization of the yeast homeodomain transcriptional regulators alpha 2 and a1 induces an interfacial helix in alpha 2.

    abstract：:The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00197a033

    authors： Phillips CL,Stark MR,Johnson AD,Dahlquist FW

    更新日期：1994-08-09 00:00:00

  • Organization and interaction of cholesterol and phosphatidylcholine in model bilayer membranes.

    abstract：:The molecular organization of sterols in liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at 37 degrees C is examined by utilizing the fluorescent analogue of cholesterol cholesta-5,7,9-trien-3 beta-ol (cholestatrienol). (1) Cholestatrienol is shown to be indistinguishable from native cholesterol i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00456a027

    authors： Hyslop PA,Morel B,Sauerheber RD

    更新日期：1990-01-30 00:00:00

  • "Nonclassical" secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane.

    abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355239

    authors： Danielsen EM,van Deurs B,Hansen GH

    更新日期：2003-12-16 00:00:00

  • A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.

    abstract：:We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500500h

    authors： Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An J

    更新日期：2014-08-05 00:00:00

  • A metastable state of Torpedo californica acetylcholinesterase generated by modification with organomercurials.

    abstract：:Chemical modification of Torpedo californica acetylcholinesterase by various sulfhydryl reagents results in its conversion to one of two principal states. One of these states, viz., that produced by disulfides and alkylating agents, is stable. The second state, produced by mercury derivatives, is metastable. At room t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00252a006

    authors： Kreimer DI,Dolginova EA,Raves M,Sussman JL,Silman I,Weiner L

    更新日期：1994-12-06 00:00:00