Inhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase.

abstract：:While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

journal_title：Biochemistry

authors： Xin X,Xi L,Tu SC

更新日期：1994-10-11 00:00:00

abstract：:To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...

journal_title：Biochemistry

authors： Wu Z,Ruoho AE

更新日期：2000-10-24 00:00:00

abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

journal_title：Biochemistry

authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

更新日期：1993-12-14 00:00:00

abstract：:We have employed a combination of gel retardation, protein-DNA cross-linking, and protein-protein cross-linking techniques to further examine the 2,3,7,8-tetrachlorodibenzo-p- dioxin-(TCDD-) dependent changes in the Ah receptor that result in a DNA-binding conformation. Gel retardation analysis of DNA-Sepharose chroma...

journal_title：Biochemistry

authors： Gasiewicz TA,Elferink CJ,Henry EC

更新日期：1991-03-19 00:00:00

abstract：:Homoisocitrate dehydrogenase (HIcDH, 3-carboxy-2-hydroxyadipate dehydrogenase) catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD as an oxidizing agent. A chemical mechanism for HIcDH is proposed on the basis of the p...

journal_title：Biochemistry

authors： Lin Y,Volkman J,Nicholas KM,Yamamoto T,Eguchi T,Nimmo SL,West AH,Cook PF

更新日期：2008-04-01 00:00:00

abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

journal_title：Biochemistry

authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

更新日期：2011-04-12 00:00:00

abstract：:Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for leukocyte transendothelia...

journal_title：Biochemistry

authors： Marchese ME,Berdnikovs S,Cook-Mills JM

更新日期：2012-10-16 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:Ultraviolet (uv)-light-mediated formation of protein-DNA adducts in Chinese hamster cell chromatin was investigated in an attempt to compare chromatin alterations induced in vitro with those observed in vivo. Three independent methods of analysis indicated stable protein-DNA associations: (1) a membrane filter assay w...

journal_title：Biochemistry

authors： Strniste GF,Rall SC

更新日期：1976-04-20 00:00:00

abstract：:The interaction between the bacteriophage Q beta coat protein and its specific binding site on Q beta genomic RNA was characterized by using a nitrocellulose filter binding assay. Q beta coat protein bound to a synthetic 29-nucleotide RNA hairpin with an association constant of 400 microM-1 at 4 degrees C, 0.2 M ionic...

journal_title：Biochemistry

authors： Witherell GW,Uhlenbeck OC

更新日期：1989-01-10 00:00:00

abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...

journal_title：Biochemistry

authors： St-Jean M,Blonski C,Sygusch J

更新日期：2009-06-02 00:00:00

abstract：:Parathyroid hormone (PTH) regulates calcium and phosphate metabolism through a G-protein-coupled receptor which is shared with PTH-related protein (PTHrP). Therefore, structure-activity studies of PTH and PTHrP with their common receptor provide an unusual opportunity to examine the structural elements in the two horm...

journal_title：Biochemistry

authors： Nakamoto C,Behar V,Chin KR,Adams AE,Suva LJ,Rosenblatt M,Chorev M

更新日期：1995-08-22 00:00:00

abstract：:We consider the problem of color regulation in visual pigments for both bovine rhodopsin (lambda max = 500 nm) and octopus rhodopsin (lambda max = 475 nm). Both pigments have 11-cis-retinal (lambda max = 379 nm, in ethanol) as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins ...

journal_title：Biochemistry

authors： Koutalos Y,Ebrey TG,Tsuda M,Odashima K,Lien T,Park MH,Shimizu N,Derguini F,Nakanishi K,Gilson HR

更新日期：1989-03-21 00:00:00

abstract：:The Escherichia coli L-arabinose-binding protein (ABP) participates as a specific receptor in the transport of L-arabinose, D-fucose, and D-galactose through the periplasmic space. The wild-type protein binds L-arabinose about 40 times more strongly than D-fucose. A mutation of the protein at position 108 (Met-->Leu) ...

journal_title：Biochemistry

authors： Zacharias M,Straatsma TP,McCammon JA,Quiocho FA

更新日期：1993-07-27 00:00:00

abstract：:Copper, zinc superoxide dismutase is a dimeric enzyme, and it has been shown that no cooperativity between the two subunits of the dimer is operative. The substitution of two hydrophobic residues, Phe 50 and Gly 51, with two Glu's at the interface region has disrupted the quaternary structure of the protein, thus prod...

journal_title：Biochemistry

authors： Banci L,Benedetto M,Bertini I,Del Conte R,Piccioli M,Viezzoli MS

更新日期：1998-08-25 00:00:00

abstract：:Protein C inhibitor (PCI) is a versatile serine protease inhibitor with both pro- and anticoagulant and other properties. Interactions of certain ligands with PCI, including heparin, affect its specificity for proteases. In this study, heparin was found to enhance PCI inhibition of factor Xa up to 42-fold in the prese...

journal_title：Biochemistry

authors： Sun W,Eriksson AS,Schedin-Weiss S

更新日期：2009-02-10 00:00:00

abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...

journal_title：Biochemistry

authors： Li J,Xiong Y,Bigelow DJ,Squier TC

更新日期：2004-01-20 00:00:00

abstract：:We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...

journal_title：Biochemistry

authors： Zaheer A,Lim R

更新日期：1996-05-21 00:00:00

abstract：:5'-Nucleotidase has been extracted from rat heart and purified to apparent homogeneity. The enzyme is a glycoprotein. Gel electrophoresis in the presence of sodium dodecyl sulfate indicates that the apparent molecular weight of the subunit is 74 000 at several different gel concentrations. Cross-linking of the native ...

journal_title：Biochemistry

authors： Naito Y,Lowenstein JM

更新日期：1981-09-01 00:00:00

abstract：:The spontaneous hydrolyses of lactic acid O-phenyl phosphate (I) and, to a lesser extent, 3-hydroxybutyric acid O-phenyl phosphate (II) have been investigated and compared with similar intramolecular and bimolecular reactions. Compared to bimolecular nucleophilic reactions, the reactivity of II is similar to other sys...

journal_title：Biochemistry

authors： Steffens JJ,Siewers IJ,Benkovic SJ

更新日期：1975-06-03 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:Phospholipase Cβ (PLCβ) enzymes hydrolyze phosphatidylinositol 4,5-bisphosphate to produce second messengers that regulate intracellular Ca2+, cell proliferation, and survival. Their activity is dependent upon interfacial activation that occurs upon localization to cell membranes. However, the molecular basis for how ...

journal_title：Biochemistry

authors： Hudson BN,Hyun SH,Thompson DH,Lyon AM

更新日期：2017-10-17 00:00:00

abstract：:Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...

journal_title：Biochemistry

authors： Pegg AE,Wechter RS,Clark RS,Wiest L,Erwin BG

更新日期：1986-01-28 00:00:00

abstract：:The malaria parasite pigment, hemozoin, is a crystal of ferriprotoporphyrin IX (FP-Fe(III)), a product of hemoglobin digestion. Hemozoin formation is essential for FP-Fe(III) detoxification in the parasite; it is the main target of quinoline antimalarials and can modulate immune and inflammation responses. To gain fur...

journal_title：Biochemistry

authors： Klonis N,Dilanian R,Hanssen E,Darmanin C,Streltsov V,Deed S,Quiney H,Tilley L

更新日期：2010-08-10 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:Multimodular enzymes, including polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and mixed PKS/NRPS systems, contain functional domains with similar functions. Domain swapping and module fusion are potential powerful strategies for creating hybrid enzymes to synthesize modified natural products. ...

journal_title：Biochemistry

authors： Suo Z

更新日期：2005-03-29 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...

journal_title：Biochemistry

authors： Rathbun CM,Prescher JA

更新日期：2017-10-03 00:00:00

abstract：:The present study was carried out to determine the energetics of Clostridium symbiosum pyruvate phosphate dikinase (PPDK) catalyzed interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (Pi), and pyruvate (pyr) with adenosine 5'-monophosphate (AMP), inorganic pyrophosphate, and phosphoenolpyruvate (PEP) [...

journal_title：Biochemistry

authors： Mehl A,Xu Y,Dunaway-Mariano D

更新日期：1994-02-08 00:00:00

abstract：:Simultaneous replacement of Asp-94 with serine and Gly-98 with glutamate in rat alpha-parvalbumin creates a CD-site ligand array in the context of the EF-site binding loop. Previous work has shown that, relative to the wild-type CD site, this engineered site has markedly reduced Ca(2+) affinity. Seeking an explanation...

journal_title：Biochemistry

authors： Tanner JJ,Agah S,Lee YH,Henzl MT

更新日期：2005-08-23 00:00:00