Abstract：
:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin with the same affinity as did the wild-type-expressing cell line, hIRcB. The HIR delta E21 receptors did not display detectable autophosphorylation or kinase activity, and as expected, internalization was impaired and metabolic signaling properties were absent. Unexpectedly, insulin's ability to stimulate DNA synthesis in cells expressing HIR delta E21 receptors was far greater than that in the parental Rat-1 cells and equal to that measured in the hIRcB cell line. The enhanced mitogenic signaling properties of the HIR delta E21 receptors was confirmed by showing that treatment of HIR delta E21 cells with a human-specific insulin-mimetic anti-insulin receptor antibody also led to enhanced DNA synthesis. Thus, although no insulin receptor autophosphorylation or kinase activity was detectable in HIR delta E21 cells, these cells displayed enhanced insulin-induced mitogenic signaling. These results suggest that an alternative non-kinase-dependent stimulus-response pathway exists for the long-term biological effects of insulin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JMdoi
10.1021/bi00212a021subject
Has Abstractpub_date
1993-12-14 00:00:00pages
13545-50issue
49eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:myo-Inositol (mI) is widely distributed in all domains of life and is important for several cellular functions, including bacterial survival. The enzymes responsible for the bacterial catabolism of mI, encoded in the iol operon, can vary from one organism to another, and these pathways have yet to be fully characteriz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00539
更新日期：2020-08-18 00:00:00
abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7000246
更新日期：2007-06-19 00:00:00
abstract：:Modification of acyl carrier proteins (ACP) or domains by the covalent binding of a 4'-phosphopantetheine (4'-PP) moiety is a fundamental condition for activation of fatty acid synthases (FASes) and polyketide synthases (PKSes). Binding of 4'-PP is mediated by 4' phosphopantetheinyl transfersases (PPTases). Mycobacter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501444e
更新日期：2015-04-14 00:00:00
abstract：:Oligonucleotide-directed triple helix formation normally requires a long tract of oligopyrimidine.oligopurine sequence. This limitation can be partially overcome by alternate-strand triple helix (or switch triple helix) formation which enables recognition of alternating oligopurine/oligopyrimidine sequences. The prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980618+
更新日期：1998-09-22 00:00:00
abstract：:This paper defines the properties of interaction of endothelin-1 (Et-1) with cloned bovine ETA receptors. The Kd value of Et-1/ETA receptor complexes was estimated in membrane preparations to 20 pM using kinetic experiments and saturation experiments performed under quasi equilibrium conditions. Competition experiment...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961238w
更新日期：1996-11-26 00:00:00
abstract：:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dod...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00663a028
更新日期：1976-09-07 00:00:00
abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035348w
更新日期：2003-12-16 00:00:00
abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802336p
更新日期：2009-02-24 00:00:00
abstract：:Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00211a004
更新日期：1993-12-07 00:00:00
abstract：:Laminins are large multidomain proteins of the extracellular matrix (ECM) with important functions in the development and maintenance of cellular organization and supramolecular structure, in particular in basement membranes. Each molecule is composed of three polypeptide chains, A (300-400 kDa) and B1 and B2 (180-200...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi00159a001
更新日期：1992-11-10 00:00:00
abstract：:Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00350a022
更新日期：1986-01-28 00:00:00
abstract：:The kinetics of self-association for beef liver glutamate dehydrogenase (EC 1.4.1.3) have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00579a007
更新日期：1979-06-12 00:00:00
abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00312a029
更新日期：1984-08-14 00:00:00
abstract：:A variant of the Tetrahymena ribozyme that efficiently cleaves single-stranded DNA under simulated physiological conditions [Tsang, J., & Joyce, G. F. (1994) Biochemistry 33, 5966-5973] was evaluated as a potential therapeutic agent on the basis of its ability to cleave synthetic oligonucleotide substrates correspondi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960845g
更新日期：1996-09-10 00:00:00
abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a037
更新日期：1989-01-10 00:00:00
abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a002
更新日期：1992-07-21 00:00:00
abstract：:To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00525a030
更新日期：1981-10-27 00:00:00
abstract：:The sex steroid binding protein (SBP) of human pregnancy serum was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5alpha-dihydrotestosterone-17beta-succinyldiaminoethyl-(1,4-butanediol diglycidyl ether)-agarose, and preparative polyacrylamide gel electrophor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00601a008
更新日期：1978-04-18 00:00:00
abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00402a012
更新日期：1988-01-26 00:00:00
abstract：:A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972266x
更新日期：1998-02-24 00:00:00
abstract：:The low-frequency FeCN vibrations of cyanoferric myeloperoxidase (MPO) and horseradish peroxidase (HRP) have been measured by resonance Raman spectroscopy. The ordering of the frequencies of the predominantly FeC stretching and FeCN bending normal vibrational modes in the two peroxidases differs. These normal mode vib...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00492a012
更新日期：1990-10-09 00:00:00
abstract：:In this paper the interaction of cytoplasmic CopZ and the N-terminal domain of the CopA ATPase from Bacillus subtilis has been studied by NMR through (15)N-(1)H HSQC experiments in order to understand the role of the two proteins in the whole copper trafficking mechanism of the bacteria. It appears that the two protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027096p
更新日期：2003-02-25 00:00:00
abstract：:Phytate is an antinutritional factor that influences the bioavailability of essential minerals by forming complexes with them and converting them into insoluble salts. To further our understanding of the chemistry of phytate's binding interactions with biologically important metal cations, we determined the stoichiome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1010249
更新日期：2010-11-30 00:00:00
abstract：:A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00016a023
更新日期：1995-04-25 00:00:00
abstract：:Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0105476
更新日期：2001-09-25 00:00:00
abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101239r
更新日期：2011-01-25 00:00:00
abstract：:Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0155201
更新日期：2001-07-24 00:00:00
abstract：:Nucleotide-induced conformational changes of the catalytic beta subunits play a crucial role in the rotary mechanism of F(1)-ATPase. To gain insights into the energetic bases that govern the recognition of nucleotides by the isolated beta subunit from thermophilic Bacillus PS3 (Tbeta), the binding of this monomer to M...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1006767
更新日期：2010-06-29 00:00:00
abstract：:We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9813476
更新日期：1998-11-17 00:00:00
abstract：:The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00566a010
更新日期：1980-12-09 00:00:00
